FlyBase Allele Report: Dmel\MTF-1[UAS.cEa]

General Information

Symbol

Dmel\MTF-1^UAS.cEa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Egli

FlyBase ID

FBal0198056

Feature type

allele

Associated gene

Dmel\MTF-1

Associated Insertion(s)

Carried in Construct

P{UAS-MTF-1.E}

Also Known As

UAS-MTF-1

Key Links

Transgenic product class

wild_type

(Egli et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Egli et al., 2006)

Mutagen

in vitro construct

(Egli et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-MTF-1.E}

Cytology

Description

UASt regulatory sequences drive expression of MTF-1.

(Egli et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

MTF-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal oxidative stress response, with Scer\GAL4^Toll-6-D42

(Bahadorani et al., 2010)

abnormal starvation stress response, with Scer\GAL4^bbg-C96

(Bahadorani et al., 2010)

abnormal starvation stress response, with Scer\GAL4^Toll-6-D42

(Bahadorani et al., 2010)

chemical resistant, with MTF-1^140-1R, Scer\GAL4^NP2020

(Bahadorani et al., 2010)

chemical sensitive, with MTF-1^140-1R, Scer\GAL4^NP2020

(Bahadorani et al., 2010)

chemical sensitive, with MTF-1^140-1R, Scer\GAL4^NP6219

(Bahadorani et al., 2010)

chemical sensitive, with Scer\GAL4^NP2020

(Bahadorani et al., 2010)

chemical sensitive, with Scer\GAL4^Toll-6-D42

(Bahadorani et al., 2010)

long lived | chemical conditional, with Scer\GAL4^bbg-C96

(Bahadorani et al., 2010)

long lived | chemical conditional, with Scer\GAL4^Toll-6-D42

(Bahadorani et al., 2010)

short lived, with Scer\GAL4^NP6219

(Bahadorani et al., 2010)

short lived | calorie restriction conditional, with Scer\GAL4^bbg-C96

(Bahadorani et al., 2010)

short lived | calorie restriction conditional, with Scer\GAL4^Toll-6-D42

(Bahadorani et al., 2010)

short lived | chemical conditional, with MTF-1^140-1R, Scer\GAL4^NP2020

(Bahadorani et al., 2010)

short lived | chemical conditional, with MTF-1^140-1R, Scer\GAL4^NP6219

(Bahadorani et al., 2010)

short lived | chemical conditional, with Scer\GAL4^NP2020

(Bahadorani et al., 2010)

short lived | chemical conditional, with Scer\GAL4^Toll-6-D42

(Bahadorani et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Overexpression of MTF-1^Scer\UAS.cEa in the gut under the control of Scer\GAL4^NP2020 moderately increases resistance to iron and cadmium toxicity in an MTF-1^140-1R mutant background but sensitises flies to zinc supplementation.

Overexpression of MTF-1^Scer\UAS.cEa in hemocytes under the control of Scer\GAL4^clu-NP6219 fully rescues the cadmium-sensitive phenotype of MTF-1^140-1R mutant flies but increases sensitivity to zinc toxicity.

Overexpression of MTF-1^Scer\UAS.cEa in the peripheral nervous system (PNS) using Scer\GAL4^bbg-C96 and motorneurons using Scer\GAL4^D42 significantly extends life-span on normal food or metal-supplemented media (i.e supplemented with cadmium or iron).

Overexpression of MTF-1^Scer\UAS.cEa in motorneurons using Scer\GAL4^D42 shortens life-span upon zinc supplementation. On the other hand, MTF-1^Scer\UAS.cEa overexpression in the PNS extends life-span on zinc supplementation.

Overexpression of MTF-1^Scer\UAS.cEa in the PNS under the control of Scer\GAL4^D42 enhances resistance to 100% oxygen compared with wild-type.

Overexpression of MTF-1^Scer\UAS.cEa in the nervous system using either Scer\GAL4^D42 or Scer\GAL4^bbg-C96 sensitises flies to starvation-induced mortality.

(Bahadorani et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

MTF-1^UAS.cEa, Scer\GAL4^GMR.PF is a suppressor of visible | conditional phenotype of Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

MTF-1^UAS.cEa, Scer\GAL4^GMR.PF is a suppressor of eye | conditional phenotype of Ctr1B^UAS.cSa, Scer\GAL4^GMR.PF

(Egli et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype caused by expression of Ctr1B^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF in the presence of 20μM copper is suppressed by co-expression of MTF-1^Scer\UAS.cEa.

(Egli et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

MTF-1^UAS.cEa/Scer\GAL4^NP2020 partially rescues MTF-1^140-1R

(Bahadorani et al., 2010)

Scer\GAL4^NP6219/MTF-1^UAS.cEa partially rescues MTF-1^140-1R

(Bahadorani et al., 2010)

Comments

(Bahadorani et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

MTF-1^Scer\UAS.cEa

MTF-1^UAS.cEa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Egli

Secondary FlyBase IDs

References (3)

Report Sections

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