FB2024_03 , released June 25, 2024
Allele: Dmel\aPKCDN.UAS.Tag:CAAX(Unk)
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General Information
Symbol
Dmel\aPKCDN.UAS.Tag:CAAX(Unk)
Species
D. melanogaster
Name
FlyBase ID
FBal0189627
Feature type
allele
Associated gene
Dmel\aPKC
Associated Insertion(s)
Carried in Construct
P{UAS-aPKC.DN.CAAX}
Key Links
Transgenic product class
dominant_negative_variant
(Sotillos et al., 2004)
missense_variant
(Sotillos et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Sotillos et al., 2004)
missense_variant
(Sotillos et al., 2004)
Mutagen
in vitro construct
(Sotillos et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-aPKC.DN.CAAX}
Cytology
Description

UASt regulatory sequences drive expression of a membrane-targeted (by the CAAX motif) kinase-dead aPKC sequence. Carries the amino acid replacement K293W.

(Sotillos et al., 2004)

UASt regulatory sequences drive expression of a dominant-negative, kinase-dead form of aPKC (carries a K293W amino acid substitution in the ATP binding site) that is targeted to the membrane due to the presence of a Tag:CAAX(Unk) tag.

(Sotillos et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
aPKC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of aPKCCAAXDN.Scer\UAS under the control of Scer\GAL4Bx-MS1096 has no effect on growth or survival of the wing discs.

      (Skwarek et al., 2014)

      Expression of aPKCCAAXDN.Scer\UAS under the control of Scer\GAL4wor.PA has no affect on the number of neuroblasts in the larval brain.

      (Lee et al., 2006)

      Overexpression of aPKCCAAXDN.Scer\UAS, under the control of Scer\GAL4en-e16E results in the loss of membrane associated crb and Patj concomitant with an apical expansion of basolateral markers.

      (Sotillos et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of aPKCCAAXDN.Scer\UAS under the control of Scer\GAL4Bx-MS1096 suppresses the tumorous growth seen in slmbUU11 mutant wing disc clones. The resulting clones are smaller than in wild type.

      (Skwarek et al., 2014)

      Coexpression of aPKCCAAXDN.Scer\UAS partially suppresses both the polarity and proliferation phenotypes of wing discs expressing Syx7dsRNA.Scer\UAS.cLa under the control of Scer\GAL4en-e16E.

      Coexpression of aPKCCAAXDN.Scer\UAS partially suppresses the early pupal lethality associated with expression of Syx7dsRNA.Scer\UAS.cLa under the control of Scer\GAL4en-e16E, increasing survival of pharate adults from 30% to 91%.

      (Lu and Bilder, 2005)

      Expression of kinase-dead aPKCCAAXDN.Scer\UAS, under the regulation of Scer\GAL4en-e16E largely normalises the effect caused by overexpression of wild-type crbintra.Scer\UAS (under the control of Scer\GAL4en-e16E). Expression of kinase-dead aPKCCAAXDN.Scer\UAS, under the regulation of Scer\GAL4en-e16E normalises the effect caused by overexpression of wild-type crbT6DT9D.intra.Scer\UAS (under the control of Scer\GAL4en-e16E) in approximately 40% of the flies, even though these flies still display strong cuticular defects.

      (Sotillos et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      aPKCCAAXDN.Scer\UAS
      aPKCCAAXDN.UAS
      aPKCDN.UAS.Tag:CAAX(Unk)
      Name Synonyms
      Secondary FlyBase IDs
        References (10)