FB2024_03 , released June 25, 2024
Allele: Dmel\H15nmr-614
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General Information
Symbol
Dmel\H15nmr-614
Species
D. melanogaster
Name
FlyBase ID
FBal0183342
Feature type
allele
Associated gene
Dmel\H15
Associated Insertion(s)
Carried in Construct
Also Known As
nmr1614
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Qian et al., 2005)
Mutagen
Delta2-3 transposase
(Qian et al., 2005)
Progenitor genotype
P{PZ}H-15
(Qian et al., 2005)
Cytology
Description

Imprecise excision of the P{PZ} element, resulting in a 3941bp deletion of H15, from -331bp to +3610bp, which includes the 5'UTR, transcription and translation start sites and part of the DNA binding domain.

(Qian et al., 2005)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2L:5,404,011..5,407,951 [+]
Comment:

Inferred boundaries of a 3,941 bp deletion resulting from the imprecise excision of P{PZ}H-15, which is reported to extend from the site of the insertion 331 bp upstream of the H15 transcription start site to 3,610 bases beyone the start site. The start site was not unambiguously defined.

(Qian et al., 2005)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cardiomyopathy
      CEA
      (Qian et al., 2008)
      model of  congenital heart disease
      CEA
      (Qian et al., 2008)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      H15nmr-614 homozygotes exhibit a dramatic increase in the rate of pacing-induced heart arrest/fibrillation, with further increases with age. However,H15nmr-614 heterozygotes do not display a significant increase in heart arrest/fibrillation following pacing compared to wild-type.

      (Qian et al., 2008)

      Only 5% of H15nmr-614 stage 16/17 embryos show defects in heart tube morphogenesis.

      (Qian et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      H15nmr-614/H15[+] is a non-enhancer of adult heart phenotype of pnrVX6

      (Qian and Bodmer, 2009)

      H15nmr-614/H15[+] is a non-enhancer of adult heart phenotype of pnr1

      (Qian and Bodmer, 2009)

      H15nmr-614/H15[+] is a non-enhancer of adult heart phenotype of pnrVX1e

      (Qian and Bodmer, 2009)
      NOT Suppressor of
      Statement
      Reference

      H15nmr-614/H15[+] is a non-suppressor of adult heart phenotype of pnrVX1e

      (Qian and Bodmer, 2009)

      H15nmr-614/H15[+] is a non-suppressor of adult heart phenotype of pnr1

      (Qian and Bodmer, 2009)

      H15nmr-614/H15[+] is a non-suppressor of adult heart phenotype of pnrVX6

      (Qian and Bodmer, 2009)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increase in heart arrest/fibrillation upon electrical pacing that is seen in pnrVX1e/+, pnr1/+ or pnrVX6/+ adults is not significantly altered by H15nmr-614/+.

      (Qian and Bodmer, 2009)

      The heart morphogenesis defect of embryos expressing middsRNA.Scer\UAS under the control of both Scer\GAL4how-24B and Scer\GAL4twi.PG is enhanced in a H15nmr-614 background, with a greater number of embryos exhibiting misalignment of the myocardial cells. Additionally, these double mutant embryos show a reduction in the number of myocardial cells and a concomitant increase in pericardial cells at stage 14, a phenotype not seen either in H15nmr-614 or Scer\GAL4how-24B,Scer\GAL4twi.PG > middsRNA.Scer\UAS single mutants.

      (Qian et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      H15nmr-614
      nmr1614
      (Qian and Bodmer, 2009, Qian et al., 2008)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)