FlyBase Allele Report: Dmel\ine[RA.UAS]

General Information

Symbol

Dmel\ine^RA.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0179409

Feature type

allele

Associated gene

Dmel\ine

Associated Insertion(s)

Carried in Construct

P{UAS-ine-RA}

Also Known As

UAS-ine-RA

Key Links

Transgenic product class

cDNA

(Huang and Stern, 2002)

wild_type

(Huang and Stern, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Huang and Stern, 2002)

wild_type

(Huang and Stern, 2002)

Mutagen

in vitro construct

(Huang and Stern, 2002)

Progenitor genotype

Carried in construct

P{UAS-ine-RA}

Cytology

Description

UASt regulatory sequences drive expression of a full length ine-RA cDNA.

(Huang and Stern, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

ine

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

paralytic | heat sensitive, with Scer\GAL4^Gli-rL82

(Huang and Stern, 2002)

paralytic | heat sensitive, with Scer\GAL4^MZ1580

(Huang and Stern, 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of ine^RA.Scer\UAS under the regulation of Scer\GAL4^MZ1580 confers reduced excitability on the larval stage motor neuron, with a decreased rate of onset of long-term facilitation. Expression of ine^RA.Scer\UAS under the regulation of Scer\GAL4^MZ1580 confers temperature sensitive paralysis. 92% of flies carrying both ine^RA.Scer\UAS and Scer\GAL4^Gli-rL82 constructs become paralysed after transfer from 18^oC to 38^oC, whereas flies carrying either ine^RA.Scer\UAS or Scer\GAL4^Gli-rL82 do not show this paralysis. Only 4.5% of flies expressing ine^RA.Scer\UAS under the regulation of Scer\GAL4^MZ1580 become paralysed when placed at 29^oC. Larvae in which ine^RA.Scer\UAS is expressed under the control of either Scer\GAL4^MZ1580 or Scer\GAL4^Gli-rL82 exhibit a higher frequency of failures in evoked transmitter release from larval stage motor nerve terminals when bathed in varying Ca²⁺ concentrations. When ine^RA.Scer\UAS is expressed under the control of Scer\GAL4^Gli-rL82, at the permissive temperature of 21-22^oC, larvae exhibit compound action potentials with a reduced amplitude compared to wild-type. At the restrictive temperature of 38^oC, larvae expressing ine^RA.Scer\UAS under the control of Scer\GAL4^Gli-rL82 exhibit a complete loss of compound action potentials. This loss is reversed when the temperature is lowered to a permissive range.

(Huang and Stern, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ine^RA.UAS/Scer\GAL4^Gli-rL82 is an enhancer of paralytic | heat sensitive phenotype of para⁶³

(Huang and Stern, 2002)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

*S Expression of ine^RA.Scer\UAS driven by either Scer\GAL4^Gli-rL82 or Scer\GAL4^MZ1580 fully suppresses the downturned wings phenotype of Sh¹⁴ ine¹. The Sh¹⁴ hyperexcitability phenotype, such as leg-shaking, can be suppressed by expression of ine^RA.Scer\UAS under the control of Scer\GAL4^MZ1580.

When ine^RA.Scer\UAS is expressed under the control of Scer\GAL4^Gli-rL82 in a para⁶³ mutant, 73% of the flies become paralysed at 29^oC.

At 32^oC, all para⁶³ flies expressing ine^RA.Scer\UAS under the control of Scer\GAL4^Gli-rL82 are paralysed.

(Huang and Stern, 2002)

Expression of ine^RA.Scer\UAS driven by either Scer\GAL4^Gli-rL82 or Scer\GAL4^elav.PLu fully suppresses the downturned wings phenotype of Sh¹⁴ ine¹, along with the increased rate of onset of long-term facilitation phenotype. These flies also require even more repetitive nerve stimulation compared to wild-type for the onset of long-term facilitation. Expression of ine^RA.Scer\UAS in larval stage neurons, under the regulation of Scer\GAL4^elav.PLu partially suppresses the long-term facilitation phenotype. Expression of ine^RA.Scer\UAS driven by Scer\GAL4^MZ1580 in the glia, results in 94% rescue of the Sh¹⁴ ine¹ downturned wing phenotype. The Sh¹⁴ hyperexcitability phenotype, such as leg-shaking, can be suppressed by expression of ine^RA.Scer\UAS under the control of Scer\GAL4^MZ1580. When ine^RA.Scer\UAS is expressed under the control of Scer\GAL4^Gli-rL82, in a para⁶³ mutant, 73% of the flies become paralysed at 29^oC. When para⁶³ flies, expressing ine^RA.Scer\UAS under the control of Scer\GAL4^Gli-rL82, are placed at 32^oC, all the flies are paralysed.

(Huang and Stern, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ine^RA.UAS/Scer\GAL4^Irk2.PL rescues ine²

(Luan et al., 2015)

ine^RA.UAS/Scer\GAL4^Irk2.PL rescues ine³

(Luan et al., 2015)

ine^RA.UAS/Scer\GAL4^MZ1580 rescues ine¹

(Huang and Stern, 2002)

Partially rescues

ine^RA.UAS/Scer\GAL4^elav.PLu partially rescues ine¹

(Huang and Stern, 2002)

Fails to rescue

Scer\GAL4^elav.PU/ine^RA.UAS fails to rescue ine²

(Luan et al., 2015)

ine^RA.UAS/Scer\GAL4^repo.PU fails to rescue ine²

(Luan et al., 2015)

Scer\GAL4^elav.PU/ine^RA.UAS fails to rescue ine³

(Luan et al., 2015)

ine^RA.UAS/Scer\GAL4^repo.PU fails to rescue ine³

(Luan et al., 2015)

Comments

Expression of ine^RA.Scer\UAS in the hindgut under the control of Scer\GAL4^Irk2.PL rescues the lethality seen when ine² mutant flies are raised on hypertonic medium. Expressing ine^RA.Scer\UAS in neurons (Scer\GAL4^elav.PU) or glia (Scer\GAL4^repo.PU) does not rescue the lethality.

Expression of ine^RA.Scer\UAS in the hindgut under the control of Scer\GAL4^Irk2.PL rescues the lethality seen when ine³ mutant flies are raised on hypertonic medium. Expressing ine^RA.Scer\UAS in neurons (Scer\GAL4^elav.PU) or glia (Scer\GAL4^repo.PU) does not rescue the lethality.

Expression of ine^RA.Scer\UAS in the hindgut under the control of Scer\GAL4^Irk2.PL completely rescues the reduction in hemolymph volume and total body water content seen when ine³ mutant flies are kept on a hypertonic medium.

Expression of ine^RA.Scer\UAS in the hindgut under the control of Scer\GAL4^Irk2.PL rescues the increased sensitivity to lack of water seen when ine² mutant flies. Expressing ine^RA.Scer\UAS in neurons (Scer\GAL4^elav.PU) or glia (Scer\GAL4^repo.PU) does not rescue this increased sensitivity.

(Luan et al., 2015)

Expression of ine^RA.Scer\UAS under the control of Scer\GAL4^MZ1580 rescues the increased rate of onset of long-term facilitation phenotype seen in ine¹ mutants. The rescued flies also require even more repetitive nerve stimulation compared to wild-type for the onset of long-term facilitation.

Expression of ine^RA.Scer\UAS in larval stage neurons under the regulation of Scer\GAL4^elav.PLu partially suppresses the long-term facilitation phenotype seen in ine¹ mutants.

(Huang and Stern, 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ine^RA.Scer\UAS

ine^RA.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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