FB2024_03 , released June 25, 2024
Allele: Dmel\ine1
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General Information
Symbol
Dmel\ine1
Species
D. melanogaster
Name
FlyBase ID
FBal0034811
Feature type
allele
Associated gene
Dmel\ine
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
    Nature of the Allele
    Allele class

    loss of function allele

    (Stern and Ganetzky, 1992)
    Mutagen
    Progenitor genotype
    Cytology
    Description

    No change in the coding region.

    (Soehnge et al., 1996)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        ine1 flies show significantly greater lethality than wild-type flies when maintained for 4 days on food medium containing 0.2M NaCl. The flies show essentially complete inviability after 4 days on food medium containing 0.4M NaCl (in contrast to wild type flies, where 90% survive). Viability after 4 days on food medium containing 0M or 0.1M NaCl is similar to wild type. The flies also show increased sensitivity to KCl and sorbitol compared to wild-type flies.

        (Huang et al., 2002)

        Electrophysiological defects. Hemizygotes display downturned wings and indented thorax.

        (Stern and Ganetzky, 1992)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Other
        Statement
        Reference

        Sh14, ine1 has visible phenotype

        (Huang and Stern, 2002)
        Phenotype Manifest In
        Enhanced by
        Statement
        Reference

        ine1 has CNS surface glial cell phenotype, enhanceable by Nf1P2

        (Yager et al., 2001)
        Enhancer of
        Statement
        Reference

        ine1 is an enhancer of CNS surface glial cell phenotype of Nf1P2

        (Yager et al., 2001)

        ine1 is an enhancer of CNS surface glial cell phenotype of poe1

        (Yager et al., 2001)
        NOT Suppressor of
        Statement
        Reference

        ine1 is a non-suppressor of CNS surface glial cell phenotype of poe1

        (Yager et al., 2001)
        Other
        Statement
        Reference

        Sh14, ine1 has wing phenotype

        (Huang and Stern, 2002)
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Expression of ineRA.Scer\UAS driven by either Scer\GAL4Gli-rL82 or Scer\GAL4elav.PLu fully suppresses the downturned wings phenotype of Sh14 ine1, along with the increased rate of onset of long-term facilitation phenotype. These flies also require even more repetitive nerve stimulation compared to wild-type for the onset of long-term facilitation. Expression of ineRA.Scer\UAS in larval stage neurons, under the regulation of Scer\GAL4elav.PLu partially suppresses the long-term facilitation phenotype. Suppression of the downturned wing phenotype of Sh14 ine1 occurs upon the induction of inehs.P1 by a single heat pulse immediately before eclosion. Induction of inehs.P1 after eclosion did not rescue the downturned wings phenotype. The Sh14 hyperexcitability phenotype, such as leg-shaking, can be suppressed by expression of ineRA.Scer\UAS under the control of Scer\GAL4MZ1580.

        Expression of ineRA.Scer\UAS driven by either Scer\GAL4Gli-rL82 or Scer\GAL4MZ1580 fully suppresses the downturned wings phenotype of Sh14 ine1.

        Suppression of the downturned wing phenotype of Sh14 ine1 occurs upon the induction of inehs.P1 by a single heat pulse immediately before eclosion. Induction of inehs.P1 after eclosion does not rescue the downturned wings phenotype.

        (Huang and Stern, 2002)

        The addition of ine1 to eag1 has no effect on the perineurial glial cells. The addition of Nf1P2 enhances the ine1 perineurial glial thickness phenotype.

        (Yager et al., 2001)
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Rescued by

        ine1 is rescued by ineRA.UAS/Scer\GAL4MZ1580

        (Huang and Stern, 2002)

        ine1 is rescued by inehs.P1

        (Yager et al., 2001)
        Partially rescued by

        ine1 is partially rescued by ineRA.UAS/Scer\GAL4elav.PLu

        (Huang and Stern, 2002)

        ine1 is partially rescued by ineRB.UAS/Scer\GAL4MZ1580

        (Huang and Stern, 2002)
        Comments
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        ine1
        (Borycz et al., 2018, Huang and Stern, 2002)
        Name Synonyms
        Secondary FlyBase IDs
          References (7)