FlyBase Allele Report: Dmel\AGO2[51B]

General Information

Symbol

Dmel\AGO2^51B

Species

D. melanogaster

Name

FlyBase ID

FBal0162854

Feature type

allele

Associated gene

Dmel\AGO2

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Xu et al., 2004)

Progenitor genotype

AGO2^EP3417

(Xu et al., 2004)

Cytology

Description

Imprecise excision of the P{EP} element, resulting in a 628bp deletion that removes the ATG codons of both AGO2 isoforms.

(Xu et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | progressive (with AGO2⁴¹⁴)

(Li et al., 2013)

decreased cell number | embryonic stage

(Deshpande et al., 2005)

partially lethal - majority die

(Deshpande et al., 2005)

short lived

(Li et al., 2013)

some die during embryonic stage

(Deshpande et al., 2005)

viable

(Xu et al., 2004)

Phenotype Manifest In

primordial germ cell

(Deshpande et al., 2005)

Detailed Description

Statement

Reference

In AGO2^51B mutants the RNA interference function is lost and so adult flies expressing w^dsRNA.GMR retain the red eye color.

(Chinen and Lei, 2017)

AGO2^51B homozygotes are short lived compared to controls. 2-4 day old AGO2⁴¹⁴/AGO2^51B transheterozygous adults show a mild defect in 24 hour memory after spaced training compared to wild type. This defect is markedly worse in 20 day old adults.

(Li et al., 2013)

Embryos derived from AGO2^51B mothers are largely morphologically normal and show no defects in the transport of lipid droplets. A minor fraction exhibit defects during syncytial cleavages and do not develop beyond that stage.

(Meyer et al., 2006)

Nearly 40% of AGO2^51B mutant embryos do not hatch. The total number of pole cells observed in AGO2^51B mutants is reduced compared to wild-type (12 compared to 20 per embryo). This reduction in the number of pole cells can be traced back to nuclear cycle 8-9 when migrating nuclei first enter the posterior pole plasm.

(Deshpande et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

AGO2^51B/AGO2^51B is a suppressor of increased cell death phenotype of Diap1^RNAi.UAS, Scer\GAL4^GMR.PF

(Meyer et al., 2006)

Phenotype Manifest In

Suppressor of

Statement

Reference

AGO2^51B/AGO2^51B is a suppressor of eye phenotype of Diap1^RNAi.UAS, Scer\GAL4^GMR.PF

(Meyer et al., 2006)

Other

Statement

Reference

AGO1^k08121, AGO2^51B has embryonic/first instar larval cuticle | segment polarity expression pattern phenotype

(Meyer et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Flies carrying the Ge-1^EME polymorphism still show increased resistance to Sigma virus compared to control flies that do not carry the polymorphism when the flies are also mutant for AGO2^51B.

(Cao et al., 2016)

In flies homozygous for AGO2^51B, the Scer\GAL4^GMR.PF>th^{dsRNA.Scer\UAS} small eye phenotype is completely suppressed.

Embryos from AGO1^k08121; AGO2^51B double mutant parents exhibit segment polarity defects that are not seen in either single mutant.

(Meyer et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

AGO2^51B is rescued by AGO2^+t10.15

(Chinen and Lei, 2017)

AGO2^51B is rescued by AGO2^m2

(Chinen and Lei, 2017)

Partially rescued by

AGO2^51B is partially rescued by AGO2^m1

(Chinen and Lei, 2017)

Comments

The loss of RNA interference functionality characteristic for AGO2^51B mutants and manifested by their retaining red eye color upon expression of w^dsRNA.GMR is rescued by combination with either AGO2^+t10.15 or AGO2^m2 and results in very light variegated eye pigmentation, whereas combination with AGO2^m1 leads to only partial rescue and light red eye color.

(Chinen and Lei, 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

AGO2^51B

(Brosh et al., 2022, Chinen and Lei, 2017, Habayeb et al., 2009)

Ago2^51B

(Cao et al., 2016, Li et al., 2013)

ago2^51B

(Dufourt et al., 2011, Hain et al., 2010, Tadros et al., 2007, Meyer et al., 2006, Vagin et al., 2006, Xu et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (13)

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