FlyBase Allele Report: Dmel\wisp[KG05287]

General Information

Symbol

Dmel\wisp^KG05287

Species

D. melanogaster

Name

FlyBase ID

FBal0162139

Feature type

allele

Associated gene

Dmel\wisp

Associated Insertion(s)

P{SUPor-P}wisp^KG05287

Carried in Construct

Also Known As

wisp^KG5287

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Benoit et al., 2008)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}wisp^KG05287

Cytology

Description

Insertion within the wisp coding region, 36 residues downstream of the initiation codon.

(Benoit et al., 2008)

Insertion in the coding region, 105bp downstream of the start codon.

(Cui et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:11,894,869..11,894,869 [+]

(Cai et al., 2021, Chartier et al., 2015, Benoit et al., 2008, Cui et al., 2008, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Oogenesis appears normal in homozygous females, and they lay the normal number of eggs, but embryos derived from homozygous or wisp^KG05287/Df(1)RA47 females mated to wild-type males do not show any development.

Embryos derived from wisp^KG05287/Df(1)RA47 females do not complete meiosis. In 74% of embryos, a single meiotic spindle is visible which is thin or distorted and on which the chromosomes are scattered or separated in asymmetric pools. A second small spindle is sometimes present, which is nucleated by one or several lost chromosomes (8% of embryos). Among the remaining embryos, 15% are blocked in meiosis II; two meiotic spindles (which in most cases are abnormally arranged), can be seen. Meiotic figures in the mutant embryos correspond to either abnormal metaphase I or anaphase I.

The karyosome in wisp^KG05287/Df(1)RA47 stage 8 oocytes appears normal. 79% of stage 14 wisp^KG05287/Df(1)RA47 oocytes do not maintain metaphase I arrest properly. The chromosomes separate along the spindle and do so asymmetrically, the fourth chromosome often migrating out of the spindle. The spindle is thin or irregular.

(Benoit et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

wisp^KG05287/wisp[+] is an enhancer of lethal | embryonic stage | maternal effect phenotype of orb^mel

(Benoit et al., 2008)

NOT Enhancer of

Statement

Reference

wisp^KG05287/wisp[+] is a non-enhancer of visible phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

NOT Suppressor of

Statement

Reference

wisp^KG05287/wisp[+] is a non-suppressor of visible phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

wisp^KG05287/wisp[+] is a non-enhancer of wing phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

NOT Suppressor of

Statement

Reference

wisp^KG05287/wisp[+] is a non-suppressor of wing phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The percentage of maternal effect embryonic lethality seen in embryos derived from orb^mel homozygous females is increased by heterozygosity for wisp^KG05287. The percentage of ventralised embryos is not increased by heterozygosity for one copy of wisp^KG05287.

(Benoit et al., 2008)

Xenogenetic Interactions

Statement

Reference

wisp^KG05287 does not alter the wing posture defects seen upon expression of Hsap\PABPN1^17ala.Act88F.

(Chartier et al., 2015)

Complementation and Rescue Data

Fails to complement

wisp²⁴⁸

(Cui et al., 2008)

wisp⁴¹

(Cui et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

16467

y¹ P{SUPor-P}wisp^KG05287/FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG15737^KG05287

wisp^KG05287

(Cai et al., 2021, Chartier et al., 2015)

wisp^KG5287

(Coll et al., 2018, Benoit et al., 2008)

wisp^KG

(Cai et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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