FB2024_03 , released June 25, 2024
Allele: Dmel\BursZ5569
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General Information
Symbol
Dmel\BursZ5569
Species
D. melanogaster
Name
FlyBase ID
FBal0159431
Feature type
allele
Associated gene
Dmel\Burs
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Dewey et al., 2004)
Progenitor genotype
Cytology
Description

Amino acid replacement: G148C.

(Dewey et al., 2004)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:21,770,114..21,770,114 [+]
Nucleotide change:

G21770114T

Amino acid change:

G148C | Burs-PA

Reported amino acid change:

G148C

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Dewey et al., 2004)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      BursZ5569 homozygote females reared at elevated temperatures (up to 29[o]C) exhibit a significant delay in migration of border cell clusters in the egg chambers compared to controls.

      (Anllo and Schüpbach, 2016)

      The midguts of 10-14 day old BursZ5569/BursZ5569 mutants display intestinal stem cell hyperproliferation, and abnormal epithelial multilayering, as compared to controls.

      The midguts of 10-14 day old BursZ5569/+ mutants display intestinal stem cell hyperproliferation, as compared with wild type.

      (Scopelliti et al., 2014)

      As in wild type, no bursCCAP neurons are seen in the ventral nerve cord of BursZ5569 mutant flies at 3-4 days post-eclosion.

      (Lee et al., 2013)

      The programmed cell death which is seen in wild-type wings during wing maturation after eclosion still occurs in bursZ5569/bursZ4410 and bursZ5569/Df(3R)Exel6187 flies.

      (Natzle et al., 2008)

      Homozygous, hemizygous and bursZ1091/bursZ5569 flies fail to spread their wings following eclosion and show prolonged retention of the elongate abdomen shape which is characteristic of a newly eclosed fly. By the day after eclosion, the abdomen has shortened, but it fails to taper into the normal adult shape, consistent with a failure to sclerotise properly. bursZ5569 mutant flies are delayed in melanisation, with only 40% completing the process during the 3 hours following eclosion, but they inflate their thoracic cuticle normally. bursZ1091/bursZ5569 adults are slow to pigment, with only 18% completing pigmentation within 3 hours of eclosion. These flies also have crossed postscutellar bristles.

      (Dewey et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      BursZ5569 has adult midgut phenotype, suppressible by Scer\GAL4Mef2.PU/vnKK108432

      (Scopelliti et al., 2014)

      BursZ5569 has intestinal stem cell | adult stage phenotype, suppressible by Scer\GAL4Mef2.PU/vnKK108432

      (Scopelliti et al., 2014)

      BursZ5569 has adult midgut phenotype, suppressible by Egfr[+]/Egfrt1

      (Scopelliti et al., 2014)

      BursZ5569 has intestinal stem cell | adult stage phenotype, suppressible by Egfr[+]/Egfrt1

      (Scopelliti et al., 2014)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of vnKK108432 under the control of Scer\GAL4Mef2.PU suppresses the intestinal stem cell hyperproliferation seen in BursZ5569/BursZ5569 mutants.

      Egfrt1/+ suppresses the intestinal stem cell hyperproliferation seen in BursZ5569/BursZ5569 mutants.

      Expression of EgfrDN.Scer\UAS under the control of Scer\GAL4esg.PU suppresses the intestinal stem cell hyperproliferation seen in BursZ5569/BursZ5569 mutants.

      (Scopelliti et al., 2014)

      rk4/+ ; bursZ5569/+ double heterozygous flies show no defects in wing expansion or tanning.

      (Dewey et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      5569
      (McNabb and Truman, 2006)
      BursZ5569
      burs alphaZ5569
      (Anllo and Schüpbach, 2016)
      bursZ5569
      (Melnattur et al., 2020, Lee et al., 2013, Natzle et al., 2008, Davis et al., 2007)
      bursz5569
      (Scopelliti et al., 2019, Scopelliti et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (9)