FlyBase Allele Report: Dmel\spas[UAS.cTa]

General Information

Symbol

Dmel\spas^UAS.cTa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Trotta

FlyBase ID

FBal0158619

Feature type

allele

Associated gene

Dmel\spas

Associated Insertion(s)

Carried in Construct

P{UAS-spas.T}

Also Known As

UAS-spastin, UAS-Dspastin

Key Links

Transgenic product class

cDNA

(Trotta et al., 2004)

wild_type

(Trotta et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Trotta et al., 2004)

wild_type

(Trotta et al., 2004)

Mutagen

in vitro construct

(Trotta et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-spas.T}

Cytology

Description

UASt regulatory sequences drive expression of a spas cDNA.

(Trotta et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

spas

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cytoskeleton, with Scer\GAL4^srp.Hemo

(Stramer et al., 2010)

eye, with Scer\GAL4^GMR.PU

(Orso et al., 2005)

hemocyte, with Scer\GAL4^srp.Hemo

(Stramer et al., 2010)

microtubule, with Scer\GAL4^C57

(Mao et al., 2014, Jin et al., 2009)

microtubule, with Scer\GAL4^srp.Hemo

(Stramer et al., 2010)

neuromuscular junction | larval stage, with Scer\GAL4^elav-C155

(Trotta et al., 2004)

Detailed Description

Statement

Reference

Expression of spas^Scer\UAS.cTa under the control of Scer\GAL4^C57 results in a significant decrease in perinuclear microtubule density in larval muscle cells.

(Mao et al., 2014)

Expression of spas^Scer\UAS.cTa in hemocytes under the control of Scer\GAL4^srp.Hemo prevents assembly of a normal microtubule cytoskeleton. Early developmental dispersal of hemocytes along the ventral midline in these embryos is delayed; nonetheless, most hemocytes found their way to the ventral midline by stage 14. Despite being unable to form a microtubule arm, these cells are still able to respond to wound stimuli, although with less efficiency than wild-type. These cells fail to maintain directional persistence (i.e. they do not go straight to the wounded area) but do migrate faster than in wild-type. This results in a slightly reduced number of spas-expressing cells present at a wound 1 hour after ablation compared to wild-type.

From stage 15 onwards spas^Scer\UAS.cTa-expressing hemocytes (where spas^Scer\UAS.cTa is under the control of Scer\GAL4^srp.Hemo) fail to disperse from the ventral midline. These hemocytes are unable to polarize and remain in close contact with each other at stages when they would ordinarily be exhibiting contact repulsion from one another. While wild-type hemocytes are rarely in contact with one another for more than 10 minutes, spas^Scer\UAS.cTa-expressing hemocytes frequently retain contacts for more than 15 minutes.

(Stramer et al., 2010)

Expression of spas^Scer\UAS.cTa under the control of Scer\GAL4^C57 severs microtubules.

(Jin et al., 2009)

Expression of spas^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PU produces a moderate rough eye phenotype.

(Orso et al., 2005)

Expression of spas^Scer\UAS.cTa under the control of Scer\GAL4^elav-C155 or Scer\GAL4^αTub84B.PL generally results in 100% lethality during embryonic or very early larval stages (unless a particularly weakly expressing P{UAS-spas.T} transgene is used). Expression of spas^Scer\UAS.cTa under the control of Scer\GAL4^elav-C155 (using a weakly expressing P{UAS-spas.T} transgene that allows full viability) does not have a significant effect on the total gross synaptic area at the larval neuromuscular junction. However, these animals show a significant reduction in mean excitatory junctional current (EJC) amplitude at the larval NMJ compared to controls (47.3 +/- 8.5 nA compared to 85.3 +/- 3.8 nA). This decrease in mean EJC amplitude can be rescued if the animals are pretreated with 50μM taxol before recording the current.

(Trotta et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^C57, spas^UAS.cTa has microtubule phenotype, non-suppressible by HDAC6^KO

(Mao et al., 2014)

Scer\GAL4^C57, spas^UAS.cTa has microtubule phenotype, non-suppressible by HDAC6^UAS.cDa, Scer\GAL4^C57

(Mao et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The decrease in perinuclear microtubule density which is seen in larval muscle cells expressing spas^Scer\UAS.cTa under the control of Scer\GAL4^C57 is not altered by co-expression of HDAC6^Scer\UAS.cDa or by the presence of HDAC6^KO.

(Mao et al., 2014)

When tbce^Scer\UAS.cJa and spas^Scer\UAS.cTa are co-expressed under the control of Scer\GAL4^C57, the microtubule phenotype in the muscles is similar to that of animals expressing spas^Scer\UAS.cTa alone under the control of Scer\GAL4^C57.

When tbce^{dsRNA.Scer\UAS.cJa} and spas^Scer\UAS.cTa are co-expressed under the control of Scer\GAL4^C57, the microtubule phenotype in the muscles is similar to that of animals expressing spas^Scer\UAS.cTa alone under the control of Scer\GAL4^C57.

(Jin et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

spas^UAS.cTa is rescued by spas^K467R.UAS/Scer\GAL4^GMR.PU

(Orso et al., 2005)

Comments

Expression of spas^{K467R.Scer\UAS} suppresses the rough eye phenotype seen when spas^Scer\UAS.cTa is expressed under the control of Scer\GAL4^GMR.PU.

(Orso et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

spas^Scer\UAS.cTa

spas^UAS.cTa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Trotta

(Trotta et al., 2004)

Secondary FlyBase IDs

References (8)

Report Sections

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