FlyBase Allele Report: Dmel\Pi3K92E[CAAX.UAS]

General Information

Symbol

Dmel\Pi3K92E^CAAX.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0156077

Feature type

allele

Associated gene

Dmel\Pi3K92E

Associated Insertion(s)

Carried in Construct

P{UAS-Pi3K92E.CAAX}

Also Known As

UAS-PI3K^CAAX, UAS-dp110^CAAX, PI3K^CA, UAS-PI3K, UAS-PI3K^CA, UAS-pi3k92e^CAAX, UAS-PI3K-CAAX

Key Links

Transgenic product class

Mutagen

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct - regulatory fusion

(Parks, 2004.5.12)

in vitro construct - coding region fusion

(Parks, 2004.5.12)

Progenitor genotype

Carried in construct

P{UAS-Pi3K92E.CAAX}

Cytology

Description

Pi3K92E coding sequence with a farnesylation signal (CAAX) added to the 3' end were cloned into P{Express-UAS}. The encoded protein is constitutively active. Expression is governed by UAS regulatory sequences.

(Parks, 2004.5.12)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pi3K92E

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of high grade glioma

CEA with btl::Egfr^λ.UAS

(Courant et al., 2022, Ariss et al., 2020)

CEA with Egfr^Act.cUa.UAS

(Pryszlak et al., 2021)

model of heart disease

CEA

(Karekar et al., 2021)

model of glioblastoma

CEA with btl::Egfr^λ.UAS

(Chi et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates high grade glioma

modeled by aPKC^{UAS.Tag:CAAX(Unk)}

(Paglia et al., 2017)

ameliorates Huntington's disease

modeled by Hsap\HTT^{Q138.UAS.mRFP1}

(Hansen et al., 2019)

ameliorates type 2 diabetes mellitus

modeled by Uch^KO

(Lee et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^arm.PS

(Keith et al., 2021)

abnormal neuroanatomy, with Scer\GAL4^ppk.PG

(Lin et al., 2015)

abnormal neuroanatomy | larval stage, with Scer\GAL4^ppk.PG

(Parrish et al., 2009)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

(Wong et al., 2013)

abnormal size | adult stage, with Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

decreased cell number | larval stage, with Scer\GAL4^sns.PU

(Gass et al., 2022)

increased cell size | larval stage, with Scer\GAL4^sns.PU

(Gass et al., 2022)

lethal, with Scer\GAL4^hs.PB

(Lee et al., 2024)

visible, with Scer\GAL4^ap-md544

(Lee et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing Pi3K92E^CAAX.UAS under the control of Scer\GAL4^ptc.PU leads to a significant increase in size of the wing blade domain between L3 and L4 veins.

(Kim and Choi, 2019)

Scer\GAL4^ppk.PG-mediated expression of Pi3K92E^{CAAX.Scer\UAS} in C4da neurons drives excessive dendrite branch elongation while causing little effect on patterning of C4da dendrites, resulting in dendrite arbors with a 50% increase in total dendrite length.

(Lin et al., 2015)

Expression via Scer\GAL4^wor.PA and Scer\GAL80^ase.PN does not prevent the normal shrinkage and disappearance of pupal neuroblasts.

(Homem et al., 2014)

Expression of Pi3K92E^{CAAX.Scer\UAS} under the control of two copies of Scer\GAL4^ppk.PG produces dendrite pruning defects in ddaC neurons at 16 hours after puparium formation.

(Wong et al., 2013)

Expression of Pi3K92E^{CAAX.Scer\UAS} driven by Scer\GAL4^ap-md544 in dorsal cells of the wing causes an overgrowth phenotype in which the wing bends downward.

(Lee et al., 2010)

Ectopic expression of Pi3K92E^{CAAX.Scer\UAS}-driven by Scer\GAL4^ppk.PG causes a significant increase in dendrite coverage larval hemisegments.

(Parrish et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pi3K92E^CAAX.UAS, Scer\GAL4^sns.PU has increased cell size | larval stage phenotype, suppressible by Akt^KK100495, Scer\GAL4^sns.PU

(Gass et al., 2022)

Pi3K92E^CAAX.UAS, Scer\GAL4^sns.PU has increased cell size | larval stage phenotype, suppressible by mTor^HMS01114, Scer\GAL4^sns.PU

(Gass et al., 2022)

Pi3K92E^CAAX.UAS, Scer\GAL4^ap-md544 has visible phenotype, suppressible by Sesn^d04539, Scer\GAL4^ap-md544

(Lee et al., 2010)

NOT suppressed by

Statement

Reference

Pi3K92E^CAAX.UAS, Scer\GAL4^ptc.PU has abnormal size | adult stage phenotype, non-suppressible by CCT4^KK108551, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

Suppressor of

Statement

Reference

Scer\GAL4^VGlut-OK371/Pi3K92E^CAAX.UAS is a suppressor of decreased cell number | adult stage | progressive phenotype of Uch^KO

(Lee et al., 2024)

Pi3K92E^CAAX.UAS, Scer\GAL4^Lk.2.2 is a suppressor of visible | adult stage | progressive phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^Lk.2.2

(Arnés et al., 2017)

Pi3K92E^CAAX.UAS, Scer\GAL4^e22c is a suppressor | partially of increased cell death | third instar larval stage phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^e22c

(Arnés et al., 2017)

NOT Suppressor of

Statement

Reference

Pi3K92E^CAAX.UAS, Scer\GAL4^ptc.PU is a non-suppressor of abnormal size | adult stage phenotype of CCT4^KK108551, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

Pi3K92E^CAAX.UAS, Scer\GAL4^ptc.PU is a non-suppressor of abnormal size | adult stage phenotype of CCT4^NIG.5525R, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

Pi3K92E^CAAX.UAS, Scer\GAL4^NP0001 is a non-suppressor of increased cell size phenotype of Atg9^HMS01246, Scer\GAL4^NP0001

(Wen et al., 2017)

Pi3K92E^CAAX.UAS, Scer\GAL4^en.PU is a non-suppressor of visible phenotype of Ptpmeg^UAS.cLa, Scer\GAL4^en.PU

(Li et al., 2015)

Scer\GAL4^ppk.PG/Pi3K92E^CAAX.UAS is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pi3K92E^CAAX.UAS, Scer\GAL4^sns.PU has slit diaphragm | larval stage | decreased number phenotype, suppressible | partially by Akt^KK100495, Scer\GAL4^sns.PU

(Gass et al., 2022)

Pi3K92E^CAAX.UAS, Scer\GAL4^sns.PU has slit diaphragm | larval stage | decreased number phenotype, suppressible | partially by mTor^HMS01114, Scer\GAL4^sns.PU

(Gass et al., 2022)

Pi3K92E^CAAX.UAS, Scer\GAL4^sns.PU has embryonic/larval garland cell | larval stage | decreased number phenotype, suppressible | partially by Akt^KK100495, Scer\GAL4^sns.PU

(Gass et al., 2022)

Pi3K92E^CAAX.UAS, Scer\GAL4^sns.PU has embryonic/larval garland cell | larval stage | decreased number phenotype, suppressible | partially by mTor^HMS01114, Scer\GAL4^sns.PU

(Gass et al., 2022)

Pi3K92E^CAAX.UAS, Scer\GAL4^ap-md544 has wing blade phenotype, suppressible by Sesn^d04539, Scer\GAL4^ap-md544

(Lee et al., 2010)

NOT suppressed by

Statement

Reference

Pi3K92E^CAAX.UAS, Scer\GAL4^ptc.PU has wing blade phenotype, non-suppressible by CCT4^KK108551, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

NOT Enhancer of

Statement

Reference

Pi3K92E^CAAX.UAS, Scer\GAL4^Appl.G1a is a non-enhancer of mitochondrion | larval stage phenotype of Hsap\HTT^{Q138.UAS.mRFP1}, Scer\GAL4^Appl.G1a

(Swinter et al., 2023)

Pi3K92E^CAAX.UAS, Scer\GAL4^Appl.G1a is a non-enhancer of larval segmental nerve phenotype of Hsap\HTT^{Q138.UAS.mRFP1}, Scer\GAL4^Appl.G1a

(Swinter et al., 2023)

Suppressor of

Statement

Reference

Pi3K92E^CAAX.UAS, Scer\GAL4^Lk.2.2 is a suppressor of proboscis | progressive phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^Lk.2.2

(Arnés et al., 2017)

Pi3K92E^CAAX.UAS, Scer\GAL4^Lk.2.2 is a suppressor of adult abdomen | progressive phenotype of Hsap\APP^2xAPP.SP.UAS, Scer\GAL4^Lk.2.2

(Arnés et al., 2017)

NOT Suppressor of

Statement

Reference

Pi3K92E^CAAX.UAS, Scer\GAL4^Appl.G1a is a non-suppressor of mitochondrion | larval stage phenotype of Hsap\HTT^{Q138.UAS.mRFP1}, Scer\GAL4^Appl.G1a

(Swinter et al., 2023)

Pi3K92E^CAAX.UAS, Scer\GAL4^Appl.G1a is a non-suppressor of larval segmental nerve phenotype of Hsap\HTT^{Q138.UAS.mRFP1}, Scer\GAL4^Appl.G1a

(Swinter et al., 2023)

Pi3K92E^CAAX.UAS, Scer\GAL4^ptc.PU is a non-suppressor of wing blade phenotype of CCT4^KK108551, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

Pi3K92E^CAAX.UAS, Scer\GAL4^ptc.PU is a non-suppressor of wing blade phenotype of CCT4^NIG.5525R, Scer\GAL4^ptc.PU

(Kim and Choi, 2019)

Pi3K92E^CAAX.UAS, Scer\GAL4^NP0001 is a non-suppressor of adult midgut epithelium phenotype of Atg9^HMS01246, Scer\GAL4^NP0001

(Wen et al., 2017)

Pi3K92E^CAAX.UAS, Scer\GAL4^en.PU is a non-suppressor of wing vein phenotype of Ptpmeg^UAS.cLa, Scer\GAL4^en.PU

(Li et al., 2015)

Scer\GAL4^ppk.PG/Pi3K92E^CAAX.UAS is a non-suppressor of larval multidendritic class IV neuron phenotype of path^dg50/Df(3L)BSC773

(Lin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Pi3K92E^{CAAX.Scer\UAS} does not suppress the increased midgut cell size resulting from the expression of Atg9^HMS01246 under the control of Scer\GAL4^{Myo31DF-NP0001}.

(Wen et al., 2017)

Expression of Pi3K92E^{CAAX.Scer\UAS} does not suppress the wing vein loss seen when Ptpmeg^Scer\UAS.cLa is expressed under the control of Scer\GAL4^en.PU.

(Li et al., 2015)

Dendrite growth in path^dg50/Df(3L)BSC773 animals arrests at the same value in the absence or presence of Scer\GAL4^ppk.PG-mediated expression of Pi3K92E^{CAAX.Scer\UAS}.

(Lin et al., 2015)

Co-expression of Sesn^d04539 with Pi3K92E^{CAAX.Scer\UAS} via Scer\GAL4^ap-md544 suppresses the Pi3K92E^{CAAX.Scer\UAS} hyperplastic wing phenotype.

(Lee et al., 2010)

Xenogenetic Interactions

Statement

Reference

The inflated abdomen and proboscis phenotype (that grows progressively more severe until death) induced by adult-onset expression of Hsap\APP^{2xAPP.SP.Scer\UAS} under the control of Scer\GAL4^Lk.2.2 (combined with Tub-Gal4 to limit the expression to adulthood) is completely suppressed in all flies by co-expression of Pi3K92E^{CAAX.Scer\UAS}. Similarly, the increased apoptotic activity (detected by Casp3 immunolabelling) observed in third instar larval wing discs expressing Hsap\APP^{2xAPP.SP.Scer\UAS} under Scer\GAL4^e22c is significantly suppressed by Pi3K92E^{CAAX.Scer\UAS} co-expression. Expresion of Hsap\APP^{2xAPP.SP.Scer\UAS} under Scer\GAL4^Toll-6-D42 either on its own or in combination with Pi3K92E^{CAAX.Scer\UAS} does not induce cell death in larval wing discs.

(Arnés et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8294

P{UAS-Pi3K92E.CAAX}1, y¹ w¹¹¹⁸

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pi3K92E^{CAAX.Scer\UAS}

Pi3K92E^CAAX.UAS

Name Synonyms

Secondary FlyBase IDs

References (31)

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