FB2024_03 , released June 25, 2024
Allele: Dmel\lsn5F3-8
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General Information
Symbol
Dmel\lsn5F3-8
Species
D. melanogaster
Name
FlyBase ID
FBal0151741
Feature type
allele
Associated gene
Dmel\lsn
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Martin et al., 2003)
Progenitor genotype
Cytology
Description

Amino acid replacement: R2term.

(Irion and St Johnston, 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:21,865,281..21,865,281 [+]
Nucleotide change:

C21865281T

Amino acid change:

R2term | lsn-PA

Reported amino acid change:

R2term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Irion and St Johnston, 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA
      (Herz et al., 2009)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      lsn5F3-8 somatic clones in eye-antennal imaginal discs contain enlarged, abnormal early endosomes.

      lsn5F3-8 somatic clones in eye-antennal imaginal discs show high levels of apoptosis compared to wildtype. However, non-autonomous overgrowth of the eye-antennal imaginal disc, adult eye and head occurs these animals.

      When the majority of cells in the eye-antennal imaginal discs are mutant for lsn5F3-8, striking tissue overgrowth of the disc occurs and loose cellular architecture is observed.

      lsn5F3-8 mutant somatic clones in eye-antennal imaginal discs do not proliferate very well, however the wildtype tissue immediately adjacent to the mutant clones show increased cell proliferation rates.

      (Herz et al., 2009)

      Females with homozygous germline clones do not lay eggs.

      (Irion and St Johnston, 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      lsn5F3-8 has eye | somatic clone phenotype, suppressible by N264-39

      (Herz et al., 2009)

      lsn5F3-8 has adult head | somatic clone phenotype, suppressible by N264-39

      (Herz et al., 2009)
      NOT Suppressor of
      Statement
      Reference

      lsn5F3-8 is a non-suppressor of eye phenotype of hidGMR.PG

      (Herz et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Heterozygosity for N264-39 suppresses the overgrowth of the adult eye and head that occurs in animals with somatic clones of lsn5F3-8 generated in the eye-antennal disc.

      The eye-ablation phenotype in WGMR.PG animals is not suppressed in lsn5F3-8 somatic clones generated in the eye-antennal imaginal disc.

      (Herz et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Selected as: a mutant that results in defective localisation of an Avic\GFP-stau marker in living oocytes in females containing homozygous germ-line clones.

      (Martin et al., 2003)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      lsn5F3-8
      (Irion and St Johnston, 2007, Irion and St Johnston, 2007)
      lsn5F8-3
      (Herz et al., 2009)
      vps225F3-8
      (Woodfield et al., 2013)
      vps225F8-3
      (Herz et al., 2009)
      Name Synonyms
      Secondary FlyBase IDs
        References (5)