FlyBase Allele Report: Dmel\pum[UAS.cSa]

General Information

Symbol

Dmel\pum^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Schweers

FlyBase ID

FBal0150290

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

Carried in Construct

P{UAS-pum.S}

Also Known As

UAS-pum, UAS-pumilio

Key Links

Transgenic product class

wild_type

(Schweers et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Schweers et al., 2002)

Mutagen

in vitro construct

(Schweers et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-pum.S}

Cytology

Description

UASt sequences drive expression of the pum coding sequence.

(Schweers et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

pum

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by para^bss1

(Lin et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Chen et al., 2008)

abnormal neurophysiology, with Scer\GAL4^elav-C155

(Schweers et al., 2002)

abnormal neurophysiology, with Scer\GAL4^eve.RN2O

(Mee et al., 2004)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^eve.RRa

(Lin et al., 2017)

lethal, with Scer\GAL4^elav-C155

(Chen et al., 2008)

lethal, with Scer\GAL4^GH146

(Chen et al., 2008)

lethal, with Scer\GAL4^Mef2.247

(Chen et al., 2008)

lethal, with Scer\GAL4^MJ85b

(Chen et al., 2008)

lethal, with Scer\GAL4^Tab2-201Y

(Chen et al., 2008)

paralytic | third instar larval stage, with Scer\GAL4^ChAT.7.4

(Lin et al., 2017)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^ey-OK107

(Chen et al., 2008)

Kenyon cell, with Scer\GAL4^ey-OK107

(Chen et al., 2008)

larval DA1 motor neuron, with Scer\GAL4^eve.RN2O

(Mee et al., 2004)

larval DA1 motor neuron | third instar larval stage, with Scer\GAL4^eve.RRa

(Lin et al., 2017)

larval RP2 motor neuron, with Scer\GAL4^eve.RN2O

(Mee et al., 2004)

Detailed Description

Statement

Reference

Expression of pum^Scer\UAS.cSa under the control of Scer\GAL4^ChAT.7.4 induces a seizure phenotype in third instar larvae subjected to an electroshock - the larvae become temporarily paralyzed with recovery time significantly longer compared to controls. Scer\GAL4^eve.RRa-driven expression leads to a significant reduction of the persistent voltage-gated Na[+] current (I[[NaP]]) in aCC neurons, the transient voltage-gated Na[+] current (I[[NaT]]) is not changed compared to wild-type controls and the persistent-to-transient ratio is decreased.

(Lin et al., 2017)

Expression of pum^Scer\UAS.cSa under the control of Scer\GAL4^ey-OK107 causes a severe defect in the mushroom body neuron axon projection. The mushroom body lobe structure is not formed and the Kenyon cell axons terminate prematurely just medial to the peduncle. No defects are observed when pum^Scer\UAS.cSa is expressed under the control of Scer\GAL4^238Y, Scer\GAL4^c739, Scer\GAL4^c747 or Scer\GAL4^c309.

(Chen et al., 2008)

Expression of pum^Scer\UAS.cSa under the control of Scer\GAL4^eve.RN2O results in a reduction of both transient and persistent components of the voltage-gated Na⁺ current in aCC/RP2 neurons. Endogenous membrane excitability, determined by injection of constant current, is reduced.

(Mee et al., 2004)

Expression of pum^Scer\UAS.cSa when driven by Scer\GAL4^elav-C155 prevents long term facilitation (LTF) onset at the neuromuscular junction. These larvae respond to nerve stimuli with excitatory junctional potentials (ejp's) only 20% of the time (as compared to about 80% in controls) in low Ca²⁺ (0.15mM) conditions. When pum^Scer\UAS.cSa is driven by Scer\GAL4^elav-C155 no detectable behavioural defects are seen.

(Schweers et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

pum^UAS.cSa/Scer\GAL4^ChAT.7.4 is a suppressor of paralytic | adult stage phenotype of para^bss1

(Lin et al., 2017)

pum^UAS.cSa/Scer\GAL4^ChAT.7.4 is a suppressor of paralytic | third instar larval stage phenotype of para^bss1

(Lin et al., 2017)

pum^UAS.cSa/Scer\GAL4^elav.PLu is a suppressor of paralytic | third instar larval stage phenotype of para^bss1

(Lin et al., 2017)

Scer\GAL4^eve.RRa/pum^UAS.cSa is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of para^bss1

(Lin et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^eve.RRa/pum^UAS.cSa is a suppressor of larval DA1 motor neuron | third instar larval stage phenotype of para^bss1

(Lin et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of pum^Scer\UAS.cSa under the control of Scer\GAL4^ChAT.7.4 or Scer\GAL4^elav.PLu fully suppresses the seizure-like phenotype (temporal paralysis) observed in para^bss1 mutant males subjected to brief vortexing or in mutant third instar larvae upon an electroshock and their recovery time is comparable to that of wild-type controls. Similarly, expression of pum^Scer\UAS.cSa driven by Scer\GAL4^elav.PLu significantly reduced the seizure duration in para^bss1 larvae.

para^bss1 mutant larvae expressing pum^Scer\UAS.cSa in the aCC motoneuron controlled by Scer\GAL4^eve.RRa show a striking reduction in the persistent voltage-gated Na[+] current (I[[NaP]]) in the aCC but no significant change in the transient voltage-gated Na[+] current (I[[NaT]]) relative to para^bss1 mutants alone, therefore the persistent-to-transient ration is markedly decreased, consistent with the presumed anticonvulsant role of pum upregulation. On occasion, multiple resurgent currents appear during the I[[NaP]] plateau in para^bss1 larvae and the proportion of cells showing these are decreased upon pum^Scer\UAS.cSa expression.

(Lin et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pum^Scer\UAS.cSa

pum^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Schweers

(Schweers et al., 2002)

Secondary FlyBase IDs

References (4)

Report Sections

Open Close