FlyBase Allele Report: Dmel\RYBP[KG08683]

General Information

Symbol

Dmel\RYBP^KG08683

Species

D. melanogaster

Name

FlyBase ID

FBal0148327

Feature type

allele

Associated gene

Dmel\RYBP

Associated Insertion(s)

P{SUPor-P}RYBP^KG08683

Carried in Construct

Also Known As

dRYBP¹

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(González et al., 2008)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}RYBP^KG08683

Cytology

Description

Insertion in the 5' UTR.

(González et al., 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:22,671,074..22,671,074 [-]

(Wilkinson et al., 2010, González et al., 2008, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage

(González et al., 2008)

female sterile

(González et al., 2008)

lethal | embryonic stage

(González et al., 2008)

partially lethal - majority die

(González et al., 2008)

visible

(González et al., 2008)

visible | somatic clone

(González et al., 2008)

Phenotype Manifest In

abdominal sternite 6 | male

(González et al., 2008)

leg

(González et al., 2008)

organism | embryonic stage

(González et al., 2008)

wing

(González et al., 2008)

wing | somatic clone

(González et al., 2008)

wing vein L5 | distal

(González et al., 2008)

Detailed Description

Statement

Reference

Wing discs of RYBP^KG08683 larvae show similar levels of developmental apoptosis as wild type controls.

RYBP^KG08683 embryos display no appreciable changes in cell death throughout the central nervous system during nerve cord condensation in stage 16 embryos or in the embryonic head, compared to controls.

(González and Busturia, 2009)

All the phenotypes observed in homozygous embryos, larvae and adults are highly variable both in their penetrance and expressivity and similar results are obtained in RYBP^KG08683/Df(2R)58B3-59 animals.

Homozygous animals are sub-lethal and show progressive lethality throughout development. Only 13% of homozygous embryos reach adulthood, with 43% dying during embryogenesis and 44% dying during larval/pupal development.

Homozygous larvae show a significant developmental delay; first instar larvae take 10 days to reach the pupal stage at 25[o]C (compared to 4-5 days for wild-type larvae).

90% of homozygous females are sterile, and in most cases, oogenesis is arrested at stage 8.

Homozygous adults derived from a cross of homozygous females to homozygous males show the same phenotypes as homozygous offspring of heterozygous parents.

Approximately 60% of homozygous embryos die before cuticle formation, showing severe defects in the pattern of nuclear division and of mitotic progression. The nuclear defects are asynchronous and large irregular nuclei are often formed. The remaining approximately 40% of homozygous embryos reach the stage of larval cuticle formation with no detectable cuticle defects.

21% of homozygous adults have a distal gap in the L5 vein, 11% have malformed legs, 54% have umbrella-shaped wings and 5% have 2-3 bristles on the sixth sternite in males. Homozygous flies show a 27% decrease in the size of the wings compared to wild type.

Homozygous embryos show a normal pattern of apoptosis.

Homozygous clones in the wing disc are similar in size to their wild-type twin spots.

Homozygous clones on either the dorsal or ventral adult wing surface result in the appearance of a blister on the opposite surface. The mutant clones show an increase in cell density.

(González et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

RYBP^KG08683, Sce¹ has visible | dominant phenotype

(González et al., 2008)

RYBP^KG08683, trx^E2 has visible | dominant phenotype

(González et al., 2008)

RYBP[+]/RYBP^KG08683, trx^E2 has visible | dominant phenotype

(González et al., 2008)

Phenotype Manifest In

Other

Statement

Reference

RYBP^KG08683, Sce¹ has sex comb | ectopic phenotype

(González et al., 2008)

RYBP^KG08683, Sce¹ has mesothoracic leg phenotype

(González et al., 2008)

RYBP^KG08683, Sce¹ has metathoracic leg phenotype

(González et al., 2008)

RYBP^KG08683, Sce¹ has adult abdominal segment 5 phenotype

(González et al., 2008)

RYBP^KG08683, trx^E2 has adult abdominal segment 5 phenotype

(González et al., 2008)

RYBP[+]/RYBP^KG08683, trx^E2 has adult abdominal segment 5 phenotype

(González et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

68% of RYBP^KG08683/RYBP^KG08683 ; Sce¹/+ males show depigmentation of the fifth abdominal segment. 42% of the males have sex combs on the T2 or T3 legs.

52% of RYBP^KG08683/+ ; trx^E2/+ males show depigmentation of the fifth abdominal segment. 95% of RYBP^KG08683/RYBP^KG08683 ; trx^E2/+ males show depigmentation of the fifth abdominal segment.

(González et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

14968

y¹ w^67c23; P{SUPor-P}RYBP^KG08683/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG12190^KG08683

RYBP^KG08683

(Kang et al., 2022)

dRYBP¹

(Basu et al., 2014, Aparicio et al., 2013, González and Busturia, 2009, González et al., 2008)

dRYBP^KG08683

(Basu et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

Open Close