FlyBase Allele Report: Dmel\Clk[UAS.cZa]

General Information

Symbol

Dmel\Clk^UAS.cZa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Zhao

FlyBase ID

FBal0148098

Feature type

allele

Associated gene

Dmel\Clk

Associated Insertion(s)

Carried in Construct

P{UAS-Clk.Z}

Also Known As

UAS-Clk

Key Links

Transgenic product class

cDNA

(Zhao et al., 2003)

wild_type

(Zhao et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zhao et al., 2003)

wild_type

(Zhao et al., 2003)

Mutagen

in vitro construct

(Zhao et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Clk.Z}

Cytology

Description

UASt sequences drive expression of a Clk cDNA.

(Zhao et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Clk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^cry.PE

(Zhao et al., 2003)

abnormal circadian rhythm, with Scer\GAL4^cry.PZ

(Zhao et al., 2003)

abnormal locomotor rhythm, with Scer\GAL4^cry.PE

(Zhao et al., 2003)

abnormal locomotor rhythm, with Scer\GAL4^cry.PZ

(Zhao et al., 2003)

abnormal locomotor rhythm | adult stage, with Scer\GAL4^P2.4.Pdf

(Beckwith et al., 2017)

lethal, with Scer\GAL4^unspecified

(Zhao et al., 2003)

viable, with Scer\GAL4^cry.PE

(Zhao et al., 2003)

viable, with Scer\GAL4^cry.PZ

(Zhao et al., 2003)

viable, with Scer\GAL4^MJ162A

(Zhao et al., 2003)

viable, with Scer\GAL4^P2.4.Pdf

(Zhao et al., 2003)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing Clk^Scer\UAS.cZa under the control of Scer\GAL4^P2.4.Pdf show similar locomotor activity period under free-running conditions (total darkness) as controls but the percentage as well as the rhythmic power of the behavioral oscillations is somewhat decreased.

(Beckwith et al., 2017)

When Clk^Scer\UAS.cZa is driven by Scer\GAL4^cry.PP, females have a aberrant circadian rhythm. Instead of the characteristic bimodal profile, the diurnal pattern has only a single peak dominating the light phase with little or no evening peak. Mutants have a shortened period. When Clk^Scer\UAS.cZa is driven by Scer\GAL4^cry.PE, mutants have an altered diurnal behaviour. There is a single peak of activity around the time of lights-on and little or no evening peak.

(Zhao et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Clk^UAS.cZa, Scer\GAL4^cry.PZ has abnormal circadian rhythm phenotype, non-enhanceable by Pdf⁰¹

(Zhao et al., 2003)

NOT suppressed by

Statement

Reference

Clk^UAS.cZa, Scer\GAL4^cry.PZ has abnormal circadian rhythm phenotype, non-suppressible by Pdf⁰¹

(Zhao et al., 2003)

Suppressor of

Statement

Reference

Clk^UAS.cZa, Scer\GAL4^P2.4.Pdf is a suppressor | partially of abnormal circadian rhythm phenotype of Scer\GAL4^P2.4.Pdf, shn^UAS.cMa

(Beckwith et al., 2013)

NOT Suppressor of

Statement

Reference

Clk^UAS.cZa, Scer\GAL4^P2.4.Pdf is a non-suppressor of abnormal locomotor rhythm | adult stage phenotype of Scer\GAL4^P2.4.Pdf, Srrm1^KK108396

(Beckwith et al., 2017)

Clk^UAS.cZa/Scer\GAL4^P2.4.Pdf is a non-suppressor of abnormal locomotor rhythm phenotype of tyf^ΔG14151

(Lim et al., 2011)

Clk^UAS.cZa/Scer\GAL4^P2.4.Pdf is a non-suppressor of abnormal locomotor rhythm phenotype of tyf^e00614

(Lim et al., 2011)

Other

Statement

Reference

Clk^UAS.cZa, Scer\GAL4^P2.4.Pdf, shn^UAS.cMa has abnormal circadian rhythm phenotype

(Beckwith et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The loss of rhythmicity in locomotor activity under free-running (constant darkness) conditions characteristic for flies expressing SRm160^KK108396 (and UAS-dicer2 to enhance the RNAi efficiency) under the control of Scer\GAL4^P2.4.Pdf cannot be rescued by co-expression of Clk^Scer\UAS.cZa.

(Beckwith et al., 2017)

Co-expression of Clk^Scer\UAS.cZa with shn^Scer\UAS.cMa under the control of Scer\GAL4^P2.4.Pdf results in an intermediate phenotype, with a shorter period than in Scer\GAL4^P2.4.Pdf->shn^Scer\UAS.cMa flies, but a longer period than in Scer\GAL4^P2.4.Pdf->Clk^Scer\UAS.cZa flies.

(Beckwith et al., 2013)

The weakened rhythmicity seen in tyf^ΔG14151 and tyf^e00614 flies under constant darkness conditions is not suppressed by expression of Clk^Scer\UAS.cZa under the control of Scer\GAL4^P2.4.Pdf.

(Lim et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Clk^Scer\UAS.cZa

Clk^UAS.cZa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Zhao

(Zhao et al., 2003)

Secondary FlyBase IDs

References (7)

Report Sections

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