FlyBase Allele Report: Dmel\Rca1[UAS.Tag:HA]

General Information

Symbol

Dmel\Rca1^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0147570

Feature type

allele

Associated gene

Dmel\Rca1

Associated Insertion(s)

Carried in Construct

P{UAS-Rca1.HA}

Also Known As

UAS-HA-rca1

Key Links

Transgenic product class

wild_type

(Grosskortenhaus and Sprenger, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Grosskortenhaus and Sprenger, 2002)

Mutagen

in vitro construct

(Grosskortenhaus and Sprenger, 2002)

Progenitor genotype

Carried in construct

P{UAS-Rca1.HA}

Cytology

Description

Expression of Rca1 tagged with Tag:HA is under the control of UAS sequences.

(Grosskortenhaus and Sprenger, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Rca1

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage, with Scer\GAL4^arm.PS

(Grosskortenhaus and Sprenger, 2002)

some die during pupal stage, with Scer\GAL4^arm.PS

(Grosskortenhaus and Sprenger, 2002)

viable, with Scer\GAL4^prd.RG1

(Grosskortenhaus and Sprenger, 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

No mitotic defects are seen in stage 11 Rca1^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^prd.RG1 or Rca1^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^arm.PS embryos.

(Grosskortenhaus and Sprenger, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rca1^UAS.Tag:HA, Scer\GAL4^prd.RG1 is a suppressor of abnormal mitotic cell cycle phenotype of Scer\GAL4^prd.RG1, fzr^UAS.cSa

(Grosskortenhaus and Sprenger, 2002)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rca1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^prd.RG1 partially rescues the cell division defects seen in CycA^C8LR1 embryos; there is some increase in cell number in the Scer\GAL4^prd.RG1 expression domain.

(Dienemann and Sprenger, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^prd.RG1/Rca1^UAS.Tag:HA partially rescues Rca1³/Rca1²

(Grosskortenhaus and Sprenger, 2002)

Comments

In Rca1²/Rca1³; Rca1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^prd.RG1 embryos, mitosis 16 is rescued in alternating segments resulting in almost twice as many cells in mutant segments as in rescued ones.

(Grosskortenhaus and Sprenger, 2002)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rca1^{Scer\UAS.T:Ivir\HA1}

Rca1^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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