FlyBase Allele Report: Dmel\Rca1[2]

General Information

Symbol

Dmel\Rca1²

Species

D. melanogaster

Name

FlyBase ID

FBal0058351

Feature type

allele

Associated gene

Dmel\Rca1

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

nucleus | somatic clone

(Zielke et al., 2006)

wing disc | somatic clone

(Zielke et al., 2006)

Detailed Description

Statement

Reference

Rca1² mutant animals fail to execute mitosis after the 16th cell cycle, and subsequently embryos have fewer cells compared to wild-type.

Rca1² mutant clones generated in the wing imaginal disc have a severe proliferation disadvantage, and are much reduced in size compared to wild-type clones. Mutant cells undergo endoreplication of the DNA, and have an increased nuclear size.

(Zielke et al., 2006)

Rca1²/Rca1³ embryos exhibit a failure of cell division in the epidermis at mitotic cycle 16: No mitotic spindles are seen at the time when wild-type cells would normally undergo this division, and the DNA is decondensed, suggesting arrest in interphase. Rca1² homozygotes also exhibit cycle 16 cell cycle arrest. Somatic clones of Rca1² in the wing disc are smaller than their corresponding wild-type twinspots. The presence of twinspots in the absence of a corresponding Rca1² mutant clone suggests that mutant cells are sometimes eliminated from the disc.

(Grosskortenhaus and Sprenger, 2002)

Rca1²/Rca1³ transheterozygous embryos arrest in cell cycle 16.

(Dong et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rca1² has abnormal mitotic cell cycle phenotype, enhanceable by CycA^C8LR1/CycA^C8LR1

(Grosskortenhaus and Sprenger, 2002)

Suppressed by

Statement

Reference

Rca1² has abnormal mitotic cell cycle phenotype, suppressible by Df(1)bi-D3/Df(1)bi-D3

(Grosskortenhaus and Sprenger, 2002)

Rca1² has abnormal mitotic cell cycle phenotype, suppressible by Scer\GAL4^prd.RG1/CycE^UAS.cLa

(Grosskortenhaus and Sprenger, 2002)

Rca1² has abnormal mitotic cell cycle phenotype, suppressible by Scer\GAL4^prd.RG1/CycA^UAS.Tag:HA

(Grosskortenhaus and Sprenger, 2002)

Rca1² has decreased occurrence of cell division | embryonic stage phenotype, suppressible by Scer\GAL4^prd.RG1/CycA^UAS.Tag:HA

(Grosskortenhaus and Sprenger, 2002)

Rca1² has decreased occurrence of cell division | embryonic stage phenotype, suppressible by Df(1)bi-D3/Df(1)bi-D3

(Grosskortenhaus and Sprenger, 2002)

Enhancer of

Statement

Reference

Rca1²/Rca1² is an enhancer of abnormal mitotic cell cycle phenotype of CycA^C8LR1

(Grosskortenhaus and Sprenger, 2002)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

CycA^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^prd.RG1 suppresses the mitotic block due to Rca1²/Rca1², in alternate segments. CycE^Scer\UAS.cLa; Scer\GAL4^prd.RG1 suppresses the mitotic block due to Rca1²/Rca1², in alternate segments. The embryonic cycle 16 mitotic block due to Rca1²/Rca1² is suppressed by Df(1)bi-D3/Df(1)bi-D3. Cell cycle arrest in CycA^C8LR1/CycA^C8LR1; Rca1²/Rca1² embryos, occurs earlier (cycle 15) than in either single mutant (both arrest at cycle 16).

(Grosskortenhaus and Sprenger, 2002)

Xenogenetic Interactions

Statement

Reference

One copy of Rca1² suppresses the increased mitotic index seen in pupal wing clones expressing Hsp83^NIG.1242R under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} (co-expressed with Dcr-2^Scer\UAS.cDa and BacA\p35^Scer\UAS.cHa).

(Bandura et al., 2013)

Complementation and Rescue Data

Rescued by

Rca1² is rescued by Scer\GAL4^prd.RG1/Rca1^{UAS.Tag:HA.Tag:FLAG}

(Zielke et al., 2006)

Rca1² is rescued by Scer\GAL4^Tub.PU/Rca1^{UAS.Tag:HA.Tag:FLAG}

(Zielke et al., 2006)

Rca1² is rescued by Scer\GAL4^prd.RG1/Rca1^{Δ203.UAS.Tag:HA.Tag:FLAG}

(Zielke et al., 2006)

Rca1² is rescued by Scer\GAL4^prd.RG1/Rca1^{Δ133.UAS.Tag:HA.Tag:FLAG}

(Zielke et al., 2006)

Rca1² is rescued by Scer\GAL4^prd.RG1/Rca1^{ΔF-box.UAS.Tag:HA.Tag:FLAG}

(Zielke et al., 2006)

Partially rescued by

Rca1² is partially rescued by Rca1^{Δ203.UAS.Tag:HA.Tag:FLAG}/Scer\GAL4^Tub.PU

(Zielke et al., 2006)

Rca1² is partially rescued by Rca1^{ΔF-box.UAS.Tag:HA.Tag:FLAG}/Scer\GAL4^Tub.PU

(Zielke et al., 2006)

Rca1³/Rca1² is partially rescued by Scer\GAL4^prd.RG1/Rca1^UAS.Tag:HA

(Grosskortenhaus and Sprenger, 2002)

Not rescued by

Rca1² is not rescued by Scer\GAL4^prd.RG1/Rca1^{Δ255.UAS.Tag:HA.Tag:FLAG}

(Zielke et al., 2006)

Rca1² is not rescued by Scer\GAL4^prd.RG1/Rca1^{C351S.UAS.Tag:HA.Tag:FLAG}

(Zielke et al., 2006)

Comments

Expression of Rca1^{Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG}, Rca1^{Δ133.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG}, Rca1^{Δ203.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} or Rca1^{ΔF-box.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} under the control of Scer\GAL4^prd.RG1 in a Rca1² mutant background restores embryonic cell numbers to wild-type levels in Scer\GAL4^prd.RG1-expressing segments.

Expression of Rca1^{Δ255.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} or Rca1^{C351S.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} under the control of Scer\GAL4^prd.RG1 in a Rca1² mutant background fails to restore embryonic cell numbers to wild-type levels in Scer\GAL4^prd.RG1-expressing segments.

Expression of Rca1^{Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} under the control of Scer\GAL4^tub in Rca1² mutant wing disc clones suppresses the proliferation disadvantage observed in Rca1² mutant tissue, and clones are similar in size to wild-type. No endoreplication is observed in these cells and nuclear size is comparable to wild-type cells.

Expression of Rca1^{Δ203.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} or Rca1^{ΔF-box.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG} under the control of Scer\GAL4^tub in Rca1² mutant wing disc clones partially suppresses the proliferation disadvantage observed in Rca1² mutant tissue. Clones are increased in size compared to Rca1² mutant clones, but are only a third of the size of wild-type clones. Rarely, endoreplication is observed in mutant clones.

(Zielke et al., 2006)

(with Rca1³) Partially rescues: { Rca1<up>Scer\UAS. T:Ivir \HA1</up>, Scer\GAL4^prd.RG1 } In Rca1²/Rca1³; Rca1^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^prd.RG1 embryos, mitosis 16 is rescued in alternating segments resulting in almost twice as many cells in mutant segments as in rescued ones.

(Grosskortenhaus and Sprenger, 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9161

Rca1² cn¹ bw¹/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rca1²

(Bandura et al., 2013)

Rca1[2]

(Bandura et al., 2013)

rca1²

(Ricolo et al., 2016, Zielke et al., 2006, Heriche et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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