FlyBase Allele Report: Dmel\Sec5[E13]

General Information

Symbol

Dmel\Sec5^E13

Species

D. melanogaster

Name

FlyBase ID

FBal0146820

Feature type

allele

Associated gene

Dmel\Sec5

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Sommer et al., 2005)

Mutagen

ethyl methanesulfonate

(Murthy et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q361term.

(Murthy et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:3,458,610..3,458,610 [+]

Nucleotide change:

C3458610T

Amino acid change:

Q361term | Sec5-PA

Reported amino acid change:

Q361term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Murthy et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

cell lethal | recessive | somatic clone

(Murthy et al., 2003)

female fertile | germline clone

(Murthy and Schwarz, 2004)

visible | recessive

(Jiang et al., 2007)

visible | recessive | somatic clone

(Murthy et al., 2003)

visible | somatic clone

(Murthy et al., 2010)

Phenotype Manifest In

clathrin-coated vesicle & oocyte | germ-line clone

(Sommer et al., 2005)

coated pit & oocyte | germ-line clone

(Sommer et al., 2005)

cytoplasmic vesicle & oocyte | germ-line clone

(Sommer et al., 2005)

dorsal appendage | germline clone

(Murthy and Schwarz, 2004)

dorsal tracheal branch primordium

(Jiang et al., 2007)

egg | germline clone

(Sommer et al., 2005)

eye | somatic clone

(Murthy et al., 2010, Murthy et al., 2003)

Golgi stack | germline clone

(Sommer et al., 2005)

nurse cell & plasma membrane | germ-line clone

(Murthy and Schwarz, 2004)

oocyte nucleus | germline clone

(Murthy and Schwarz, 2004)

ring canal | germline clone

(Murthy and Schwarz, 2004)

yolk | germline clone

(Sommer et al., 2005)

Detailed Description

Statement

Reference

Adults in which eye tissue has been made homozygous for sec5^E13 during development (using the EGUF method) show ablation of the eye.

(Murthy et al., 2010)

sec5^E13 mutant larvae display tracheal fusion defects in 20% of the examined tracheal dorsal branches.

(Jiang et al., 2007)

Oocytes derived from sec5^E13 germline clones show the normal large increase in nurse cell and oocyte size that occurs during oogenesis, but the final eggs are flaccid and often collapse after oviposition as a result of the lack of yolk granules that fill up the wild type egg.

The amount of yolk is reduced from approximately 35% of the cytoplasm in wild type eggs to approximately 4.1% of the cytoplasm in oocytes derived from sec5^E13 germline clones.

Oocytes derived from sec5^E13 germline clones have a reduced number of vesicles and tubules beneath the oocyte surface as well as an approximately 7-fold decrease in the number of clathrin-coated pits and vesicles compared to wild type.

Both the Golgi stack and the trans-Golgi network are approximately 2-fold larger in oocytes derived from sec5^E13 germline clones compared to wild type.

(Sommer et al., 2005)

Females carrying sec5^E13 germ-line clones are fertile, but lay eggs in which the dorsal appendages are either too closely spaced or are fused. Some egg chambers in these animals lack membranes between some of the germ-cells, resulting in nurse cell nuclei falling into the oocyte and aggregation of ring canals. In some of these egg chambers the oocyte nucleus is mislocalized away from the dorsal membrane, although in all such cases the nucleus is localized in the anterior (as in wild-type).

(Murthy and Schwarz, 2004)

The eye is completely ablated in mosaic animals in which the eye is homozygous for sec5^E13. Females with homozygous germline clones do not produce eggs.

(Murthy et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Rop^G27/Rop[+], Sec5^E13 has abnormal neuroanatomy | third instar larval stage phenotype

(Peng et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Sec5^E13 is an enhancer of embryonic/first instar larval cuticle phenotype of Exo84^Z4840/Df(3R)Espl3

(Blankenship et al., 2007)

Other

Statement

Reference

Rop^G27/Rop[+], Sec5^E13 has larval multidendritic class IV neuron | third instar larval stage phenotype

(Peng et al., 2015)

EndoB⁵⁴, Sec5[+]/Sec5^E13 has oocyte phenotype

(Tsai et al., 2014)

Scer\GAL4^nanos.PG, Sec5^E13, Sec8^UASp.Tag:HA has oocyte phenotype

(Murthy et al., 2005)

Scer\GAL4^nanos.PG, Sec5^E13, Sec8^UASp.Tag:HA has nurse cell phenotype

(Murthy et al., 2005)

Scer\GAL4^nanos.PG, Sec5^E13, Sec8^UASp.Tag:HA has egg chamber phenotype

(Murthy et al., 2005)

Scer\GAL4^nanos.PG, Sec5^E13, Sec8^UASp.Tag:HA has nurse cell ring canal phenotype

(Murthy et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Sec5^E13/Rop^G27 transheterozygous third instar larvae show decreased number of terminal dendritic branches in the class IV dendritic arborizing neurons compared to either Sec5^E13/+ or Rop^G27/+ single heterozygotes.

(Peng et al., 2015)

The fraction of oocytes showing endocytic activity is reduced compared to wild type in EndoB⁵⁴/Sec5^E13 double heterozygotes.

(Tsai et al., 2014)

The cuticle defects seen at 25[o]C in embryos derived from exo84^onr/Df(3R)Espl3 females mated to exo84^onr heterozygous males are enhanced by sec5^E13 (both parents are heterozygous for the second mutation).

(Blankenship et al., 2007)

Expression of sec8^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^nos.PG in homozygous female sec5^E13 germline clones results in egg chamber development arresting between stages 7 and 9, such that these females lay no eggs. The oocyte nucleus migrates appropriately to the anterior end of the cell in the double mutant germlines at stage 7, but the oocyte fails to enlarge, remaining comparable in size to the nurse cells. Some disruption of the nurse cell membranes is seen, with some cells fused and the ring canals clustered together.

(Murthy et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sec5^E13 is rescued by Sec5^+t11.0

(Murthy et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Sec5^E13

(Peng et al., 2015, Michel et al., 2011, Sommer et al., 2005)

s5^E13

(Sommer et al., 2005)

sec5^E13

(Tsai et al., 2014, Murthy et al., 2010, Windler and Bilder, 2010, Kelkar and Dobberstein, 2009, Blankenship et al., 2007, Coutelis and Ephrussi, 2007, Jiang et al., 2007, Classen et al., 2005, Murthy et al., 2005, Sommer et al., 2005, Sommer et al., 2005)

sec5E13

(Murthy and Schwarz, 2004)

Name Synonyms

Secondary FlyBase IDs

References (15)

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