FlyBase Allele Report: Dmel\shot[LA.UAS.GFP]

General Information

Symbol

Dmel\shot^LA.UAS.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0137090

Feature type

allele

Associated gene

Dmel\shot

Associated Insertion(s)

Carried in Construct

P{UAS-shot.L(A)-GFP}

Also Known As

Shot L(A)-GFP, UAS-shot-RE-GFP, UAS-Shot L(A)-GFP, Shot::GFP, UAS-shot-GFP

Key Links

Transgenic product class

wild_type

(Lee and Kolodziej, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Lee and Kolodziej, 2002)

Mutagen

in vitro construct

(Lee and Kolodziej, 2002, Lee and Kolodziej, 2002)

Progenitor genotype

Carried in construct

P{UAS-shot.L(A)-GFP}

Cytology

Description

UAS regulatory sequences drive expression of the shot L(A) isoform tagged with GFP.

(Lee and Kolodziej, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

shot

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

terminal tracheal cell | embryonic stage, with Scer\GAL4^btl.PU

(Ricolo and Araujo, 2020)

terminal tracheal cell | larval stage, with Scer\GAL4^bs.PM

(Ricolo and Araujo, 2020)

tracheal lumen | embryonic stage | increased number, with Scer\GAL4^btl.PU

(Ricolo and Araujo, 2020)

tracheal lumen | increased number | larval stage, with Scer\GAL4^bs.PM

(Ricolo and Araujo, 2020)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

shot^LA.UAS.GFP/Scer\GAL4^elav.PLu is a suppressor of axon microtubule bundle | larval stage phenotype of Eb1⁰⁴⁵²⁴, msps^A, tau^KO

(Hahn et al., 2021)

Scer\GAL4^btl.PU/shot^LA.UAS.GFP is a suppressor of terminal tracheal cell | embryonic stage phenotype of bs⁰³²⁶⁷

(Ricolo and Araujo, 2020)

Scer\GAL4^btl.PU/shot^LA.UAS.GFP is a suppressor of tracheal lumen | embryonic stage phenotype of bs⁰³²⁶⁷

(Ricolo and Araujo, 2020)

Scer\GAL4^btl.PU/shot^LA.UAS.GFP is a suppressor of actin cytoskeleton | embryonic stage phenotype of bs⁰³²⁶⁷

(Ricolo and Araujo, 2020)

Scer\GAL4^btl.PU/shot^LA.UAS.GFP is a suppressor of microtubule | embryonic stage phenotype of bs⁰³²⁶⁷

(Ricolo and Araujo, 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^btl.PU/shot^LA.UAS.GFP rescues shot³

(Ricolo and Araujo, 2020)

shot^LA.UAS.GFP/Scer\GAL4^pnr.PU rescues shot^SF20

(Takács et al., 2017)

Scer\GAL4^en.PU/shot^LA.UAS.GFP rescues shot^SF20

(Takács et al., 2017)

Scer\GAL4^elav.PU/shot^LA.UAS.GFP rescues shot^VV/shot³

(Valakh et al., 2013)

shot^LA.UAS.GFP/Scer\GAL4^sca-537.4 rescues Df(2R)MK1/shot^SF20

(Alves-Silva et al., 2012)

Scer\GAL4^sr-md710/shot^LA.UAS.GFP rescues shot^unspecified

(Alves-Silva et al., 2012)

Scer\GAL4^unspecified/shot^LA.UAS.GFP rescues shot^unspecified

(Bottenberg et al., 2009)

Scer\GAL4^sr-md710/shot^LA.UAS.GFP rescues shot^unspecified

(Bottenberg et al., 2009)

shot^LA.UAS.GFP/Scer\GAL4^sca-537.4 rescues Df(2R)MK1/shot^SF20

(Sanchez-Soriano et al., 2009)

Scer\GAL4^insc-Mz1407/shot^LA.UAS.GFP rescues shot³

(Lee et al., 2007)

Scer\GAL4^insc-Mz1407/shot^LA.UAS.GFP rescues shot³

(Lee and Kolodziej, 2002)

shot^LA.UAS.GFP/Scer\GAL4^NP5130 rescues shot³

(Lee and Kolodziej, 2002)

Scer\GAL4^btl.PS/shot^LA.UAS.GFP rescues shot³

(Lee and Kolodziej, 2002)

Partially rescues

shot^LA.UAS.GFP/Scer\GAL4^eve.RN2O partially rescues shot^unspecified

(Alves-Silva et al., 2012)

Fails to rescue

Scer\GAL4^how-24B/shot^LA.UAS.GFP fails to rescue shot^VV/shot³

(Valakh et al., 2013)

Comments

The expression of shot^{LA.Scer\UAS.T:Avic\GFP} rescues both the increased length:width ratio of the dorsal hole (expression under the control of Scer\GAL4^pnr.PU) and the abnormal microtubules of the dorsal-most epithelial cells (expression under the control of Scer\GAL4^en.PU) observed during dorsal closure of shot^SF20 homozygous embryos.

(Takács et al., 2017)

Expression of shot^{LA.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav.PU rescues the increase in bouton number seen at the neuromuscular junction in shot³/shot^VV third instar larvae, while expression under the control of Scer\GAL4^how-24B fails to rescue the phenotype.

(Valakh et al., 2013)

Expression of shot^{LA.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^eve.RN2O significantly rescues the motor neuron stall phenotype seen in shot^unspecified embryos in vivo while expression under the control of Scer\GAL4^sca-537.4 completely rescues the axon extension and microtubule organisation defects of shot^SF20/Df(2R)MK1 embryonic neurons in primary culture.

Expression of shot^{LA.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^sr-md710 restores tendon cell integrity in shot^unspecified embryos.

(Alves-Silva et al., 2012)

Expression of shot^{LA.Scer\UAS.T:Avic\GFP} in motorneurons rescues the severe dendritic growth phenotypes of shot^unspecified embryos.

Scer\GAL4^sr-md710 driven expression of shot^{LA.Scer\UAS.T:Avic\GFP} completely rescues the shot^unspecified tendon cell phenotype.

(Bottenberg et al., 2009)

Scer\GAL4^sca-537.4-mediated expression of shot^{LA.Scer\UAS.T:Avic\GFP} fully restores the microtubule unbundling and axonal length phenotypes of shot^SF20/Df(2R)MK1 primary neuronal cultures.

Scer\GAL4^sca-537.4-mediated expression of shot^{LA.Scer\UAS.T:Avic\GFP} fully rescues the reduced filopodia phenotype of shot^SF20/Df(2R)MK1 primary neuronal cultures.

(Sanchez-Soriano et al., 2009)

Expression of shot^{LA.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4¹⁴⁰⁷ fully rescues the motor and sensory axon extension defects seen in shot³ mutants.

(Lee and Kolodziej, 2002)

Expression of shot^{LA.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^btl.PS or Scer\GAL4^esg-NP5130 rescues the anastomosis defects in the dorsal trunk, lateral trunk and dorsal midline seen in shot³ embryos.

(Lee and Kolodziej, 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29044

w^* P{UAS-shot.L(A)-GFP}1; βTub60D^Pin-1/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

shot^{LA.Scer\UAS.T:Avic\GFP}

shot^LA.UAS.GFP

Name Synonyms

Secondary FlyBase IDs

References (17)

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