FlyBase Allele Report: Dmel\sub[131]

General Information

Symbol

Dmel\sub¹³¹

Species

D. melanogaster

Name

FlyBase ID

FBal0135617

Feature type

allele

Associated gene

Dmel\sub

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Giunta et al., 2002)

Mutagen

Delta2-3 transposase

(Giunta et al., 2002)

Progenitor genotype

P{EP}sub^EP616

(Giunta et al., 2002)

Cytology

Description

Deletion from site of P{EP}sub^EP616 (located 105bp upstream of the sub ATG) removing P{EP}sub^EP616 and more than half of the sub transcription unit.

(Giunta et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | oogenesis (with sub¹)

(Radford et al., 2012)

abnormal meiotic cell cycle | recessive

(Giunta et al., 2002)

abnormal mitotic cell cycle

(Cesario et al., 2006)

abnormal mitotic cell cycle | larval stage (with sub¹)

(Cesario et al., 2006)

abnormal mitotic cell cycle | maternal effect | recessive

(Giunta et al., 2002)

female sterile (with sub¹)

(Giunta et al., 2002)

female sterile (with sub^Dub)

(Giunta et al., 2002)

female sterile | recessive

(Giunta et al., 2002)

lethal | maternal effect | recessive

(Giunta et al., 2002)

visible (with sub^Dub)

(Giunta et al., 2002)

Phenotype Manifest In

brain | larval stage

(Cesario et al., 2006)

brain | larval stage (with sub¹)

(Cesario et al., 2006)

eye (with sub^Dub)

(Giunta et al., 2002)

meiotic spindle | oogenesis (with sub²)

(Wang et al., 2021)

oocyte (with sub¹)

(Radford et al., 2012)

oocyte (with sub²)

(Wang et al., 2021)

spindle

(Giunta et al., 2002)

spindle & oocyte

(Jang et al., 2005)

spindle & oocyte (with sub¹)

(Jang et al., 2005)

spindle microtubule | larval stage

(Cesario et al., 2006)

spindle microtubule | larval stage (with sub¹)

(Cesario et al., 2006)

spindle midzone & oocyte

(Jang et al., 2005)

wing (with sub^Dub)

(Giunta et al., 2002)

wing margin (with sub^Dub)

(Giunta et al., 2002)

Detailed Description

Statement

Reference

sub¹/sub¹³¹ oocytes exhibit frequent monopolar and tripolar spindles as well as mono-orientation of homologous centromeres while they do not exhibit any failure to separate chromosomes or a defect in the movement of centromeres toward a pole present in the spindle as compared to controls.

(Radford et al., 2012)

The percentage of cells in mitosis in sub¹/sub¹³¹ mutant larval brains is approximately double that of wild-type.

sub¹/sub¹³¹ larval brains show an increased frequency of spindle assembly defects. These include frayed microtubules, unequal distribution of microtubules in the two half spindles, and disorganised or absent interpolar microtubules. 70% of mutant brains show disorganised metaphase, and 46% show lagging chromosomes at anaphase compared to 11% and 9% respectively in wild-type brains.

sub¹³¹ mutant larval brains show a higher percentage of cells in mitosis (2.66) compared to wild-type brains (1.32).

43% of sub¹³¹ mutant brains show disorganised metaphase spindles (including frayed microtubules, unequal distribution of microtubules in the two half spindles, and disorganised or absent interpolar microtubules) compared to 11% of metaphase spindles in wild-type brains. 52% show lagging chromosomes at anaphase compared to 9% in wild-type brains.

(Cesario et al., 2006)

14/17 sub¹/sub¹³¹ oocytes have abnormal spindle organisation. Among the abnormal spindles, 3 are monopolar, 9 are tripolar and 2 have other defects such as fraying of microtubules.

Most mutant oocyte spindles are not bipolar. All mutant spindles, even those that are bipolar, lack the prominent bundles of central spindle microtubules that are seen in wild-type oocytes.

(Jang et al., 2005)

Sterile females show rough eyes and clipped wings. Mutant males show elevated levels of X-Y chromosome non-disjunction in male meiosis. Embryos derived from mutant mothers show early mitotic abnormalities including arrest before embryonic mitoses begin, a polar body near one end and the development of one or two spindles in the middle. Structure of spindles in the embryos is abnormal, and their size variable.

(Giunta et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

sub¹³¹/sub¹ has abnormal mitotic cell cycle phenotype, enhanceable by polo^16-1/polo[+]

(Cesario et al., 2006)

sub¹³¹/sub¹ has abnormal mitotic cell cycle phenotype, enhanceable by Incenp^EC3747/Incenp[+]

(Cesario et al., 2006)

Suppressed by

Statement

Reference

sub¹³¹ has abnormal mitotic cell cycle phenotype, suppressible by BubR1^k03113

(Cesario et al., 2006)

sub¹³¹/sub¹ has abnormal mitotic cell cycle phenotype, suppressible by ncd¹/ncd¹

(Cesario et al., 2006)

Other

Statement

Reference

mei-38¹, sub¹³¹/sub¹ has lethal phenotype

(Wu et al., 2008)

polo^16-1, sub¹³¹/sub¹ has decreased body size | adult stage phenotype

(Cesario et al., 2006)

ncd¹, sub¹³¹/sub¹ has lethal | third instar larval stage phenotype

(Cesario et al., 2006)

BubR1^k03113, sub¹³¹ has decreased occurrence of cell division | larval stage phenotype

(Cesario et al., 2006)

polo^16-1/polo[+], sub¹³¹/sub¹ has lethal | larval stage phenotype

(Cesario et al., 2006)

ncd[+]/ncd¹, sub¹³¹/sub¹ has viable | third instar larval stage phenotype

(Cesario et al., 2006)

Incenp^EC3747/Incenp[+], sub¹³¹/sub¹ has lethal | larval stage phenotype

(Cesario et al., 2006)

Df(2L)Exel7049/+, sub¹³¹/sub¹ has lethal | larval stage phenotype

(Cesario et al., 2006)

aurA^87Ac-3/aur[+], sub¹³¹/sub¹ has viable | larval stage phenotype

(Cesario et al., 2006)

BubR1^k03113, sub¹³¹ has abnormal size | larval stage phenotype

(Cesario et al., 2006)

BubR1^k03113, sub¹³¹ has decreased cell number | larval stage phenotype

(Cesario et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

sub¹³¹/sub¹ has brain | larval stage phenotype, enhanceable by polo^16-1/polo[+]

(Cesario et al., 2006)

Suppressed by

Statement

Reference

sub¹³¹ has brain | larval stage phenotype, suppressible by BubR1^k03113

(Cesario et al., 2006)

sub¹³¹/sub¹ has brain | larval stage phenotype, suppressible by ncd¹/ncd¹

(Cesario et al., 2006)

Other

Statement

Reference

BubR1^k03113, sub¹³¹ has brain | larval stage phenotype

(Cesario et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

sub¹³¹, BubR1^k03113 double mutant larval brains show a lower percentage of cells in mitosis (0.65) compared to sub¹³¹ mutant brains (2.66) or wild-type brains (1.32).

sub¹³¹, BubR1^k03113 double mutant larval brains are slow growing, small, and contain fewer cells than BubR1^k03113 mutants.

sub¹/sub¹³¹; ncd¹/ncd¹ mutant larval brains show a comparable percentage of cells in mitosis compared to wild-type.

sub¹/sub¹³¹; polo^16-1/+ rare survivors are sickly and small with abdominal patterning defects. Late larval developmental arrest is observed. Severe defects in spindle organization are observed, and spindles are unusually short. Larvae show a higher percentage of cells in mitosis compared to sub¹/sub¹³¹ or polo^16-1/+, and nearly four times that of wild-type.

sub¹/sub¹³¹, Incenp^EC3747/+ mutant larval brains display defects in spindle assembly, and short and disorganised metaphase spindles are common. Anaphase spindles usually lack an organised midzone, and 76% show lagging chromosomes at anaphase (compared to 55% in sub¹/sub¹³¹ mutant brains). Double mutant larvae exhibit a high frequency of polyploid cells.

(Cesario et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sub¹

(Giunta et al., 2002)

sub^Dub

(Giunta et al., 2002)

Rescued by

sub¹³¹/sub¹ is rescued by sub^UASp.EGFP/Scer\GAL4^{VP16.nanos.UTR}

(Jang et al., 2007)

sub¹³¹/sub¹ is rescued by Scer\GAL4^{VP16.nanos.UTR}/sub^UASp.Tag:HA

(Jang et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allelic series (strongest to weakest phenotype): sub¹³¹/sub^Dub = sub²⁰²/sub^Dub = sub²¹⁸/sub^Dub = sub²²/sub^Dub = sub⁷²/sub^Dub = sub¹⁰⁴/sub^Dub = sub¹⁵⁸/sub^Dub > sub¹⁷⁹⁴/sub^Dub > +/sub^Dub.

(Giunta et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

sub¹³¹

(Wang et al., 2021, Das et al., 2018, Das et al., 2016, Radford et al., 2012, Wu et al., 2008, Jang et al., 2007, Cesario et al., 2006, Jang et al., 2005)

sub^null

(Giunta et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (9)

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