FlyBase Allele Report: Dmel\Rac2[EP3118]

General Information

Symbol

Dmel\Rac2^EP3118

Species

D. melanogaster

Name

FlyBase ID

FBal0134176

Feature type

allele

Associated gene

Dmel\Rac2

Associated Insertion(s)

P{EP}Rac2^EP3118

Carried in Construct

Also Known As

EP(3)3118

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bidet et al., 2003, Fauvarque et al., 2002, Pena-Rangel et al., 2002, Tseng and Hariharan, 2002)

Progenitor genotype

Associated Insertion(s)

P{EP}Rac2^EP3118

Cytology

Description

P{EP} insertion 791bp upstream of the Rac2 translation initiation codon. The P{EP} element is oriented such that expression from the element results in overexpression of Rac2.

(Bidet et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:7,435,304..7,435,304 [-]

(Morris et al., 2012, van Impel et al., 2009, Williams et al., 2005, Bidet et al., 2003, Fauvarque et al., 2002, Pena-Rangel et al., 2002, Tseng and Hariharan, 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

visible, with Scer\GAL4^GMR.PF

(Morris et al., 2012, Müller et al., 2005)

visible, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

Phenotype Manifest In

embryonic pericardial cell, with Scer\GAL4^how-24B

(Bidet et al., 2003)

embryonic somatic muscle cell, with Scer\GAL4^how-24B

(Bidet et al., 2003)

eye, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

eye, with Scer\GAL4^GMR.PF

(Müller et al., 2005)

eye, with Scer\GAL4^hs.2sev

(Tseng and Hariharan, 2002)

ommatidium, with Scer\GAL4^GMR.PF

(Morris et al., 2012)

wing, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

wing vein, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

Detailed Description

Statement

Reference

In combination with the Scer\GAL4^GMR.PF driver Rac2^EP3118 animals display rough but normal size eyes.

The Scer\GAL4^ey.PH driver produces a normal eye phenotype in Rac2^EP3118 animals.

Pan-neural expression of Rac2^EP3118 with Scer\GAL4^elav-C155 results in normal eye development.

(Morris et al., 2012)

Expression of Rac2^EP3118 under the control of Scer\GAL4^GMR.PF results in flies with slit shaped eyes.

(Müller et al., 2005)

Expression of Rac2^EP3118 under the control of Scer\GAL4^how-24B results in a reduction in the number of lateral LT muscle precursors, including the lbe-positive lateral adult muscle precursors and segmental border muscles. The number of eve-expressing pericardial cells is reduced in about 25% of these embryos, with no major changes in the number of lbe-expressing cardioblasts and pericardial cells.

(Bidet et al., 2003)

Expression of Rac2^EP3118 under the control of Scer\GAL4^ey.PH results in eyes that are strongly reduced in size. Flies expressing Rac2^EP3118 under the control of Scer\GAL4^hs.2sev have rough eyes. Flies expressing Rac2^EP3118 under the control of Scer\GAL4^en-e16E have wing venation defects and wing blisters.

(Tseng and Hariharan, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rac2^EP3118, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by CRMP^supK1

(Morris et al., 2012)

Suppressed by

Statement

Reference

Rac2^EP3118, Scer\GAL4^ey.PH has visible phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

Rac2^EP3118 is an enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PU, pbl^{DH-PH.UAS.Tag:HA}

(van Impel et al., 2009)

Rac2^EP3118, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Other

Statement

Reference

CRMP^supK1, Rac2^EP3118, Scer\GAL4^ey.PH has visible phenotype

(Morris et al., 2012)

CRMP^supK1, Rac2^EP3118, Scer\GAL4^elav-C155 has visible phenotype

(Morris et al., 2012)

Rac2^EP3118, Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+] has lethal | pupal stage phenotype

(Pena-Rangel et al., 2002)

Rac2^EP3118, Scer\GAL4^pnr-MD237, pnr^MD237 has lethal | pupal stage phenotype

(Pena-Rangel et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rac2^EP3118, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by CRMP^supK1

(Morris et al., 2012)

Suppressed by

Statement

Reference

Rac2^EP3118, Scer\GAL4^ey.PH has eye phenotype, suppressible by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

Enhancer of

Statement

Reference

Rac2^EP3118 is an enhancer of eye | adult stage phenotype of Scer\GAL4^GMR.PU, pbl^{DH-PH.UAS.Tag:HA}

(van Impel et al., 2009)

Rac2^EP3118, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

Other

Statement

Reference

CRMP^supK1, Rac2^EP3118, Scer\GAL4^ey.PH has eye phenotype

(Morris et al., 2012)

CRMP^supK1, Rac2^EP3118, Scer\GAL4^elav-C155 has eye phenotype

(Morris et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The eye phenotype resulting from the expression of Rac2^EP3118 under the control of Scer\GAL4^GMR.PF is enhanced in CRMP^supK1 animals that have reduced eyes with extensively fused ommatidia.

The Scer\GAL4^ey.PH driver produces irregular ommatidial arrangements and reduced eyes in Rac2^EP3118 animals that are homozygous for CRMP^supK1.

Pan-neural expression of Rac2^EP3118 with Scer\GAL4^elav-C155 in CRMP^supK1 homozygotes results in rough eyes.

(Morris et al., 2012)

Co-expression of Rac2^EP3118 strongly enhances the rough eye phenotype seen when pbl^{DH-PH.Scer\UAS.T:Ivir\HA1} is expressed under the control of Scer\GAL4^GMR.PU.

(van Impel et al., 2009)

Co-expression of CycE^Scer\UAS.cLa suppresses the small eye phenotype caused by expression of Rac2^EP3118 under the control of Scer\GAL4^ey.PH.

(Tseng and Hariharan, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rac2^EP3118

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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