Amino acid replacement: G1131E.
G4248277A
G1131E | ago-PA; G1131E | ago-PB; G1131E | ago-PC
G1131E
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
ago1/ago3 transheterozygous mutants develop through all embryonic stages but fail to hatch as L1 larvae.
ago3/Df(3L)Exel9000 transheterozygous mutants develop through all embryonic stages but fail to hatch as L1 larvae.
ago1/ago3 mutant embryos exhibit defects in the developing trachea. The earliest of these are interruptions or 'breaks' in the continuity of the tracheal lumen. Approximately 70% of these mutants display breaks throughout the tracheal system and are prominent in the dorsal trunk, the lateral trunk, and between dorsal branches of opposing tracheal placodes. The dorsal branches and ganglionic branches also appear to show misrouting. The second prominent tracheal phenotype (occurring in 25% of embryos) is excess lumen convolution through the primary and secondary branches. Mutants have approximately the same number of nuclei per dorsal trunk segment, indicating that the primary effect of ago loss in the dorsal trunk is not on cell number, but on fusion cell migration and fusion.
In ago3 homozygous embryos, 5% of hemisegments have two RP2 sibs and one RP2 neuron, while others have two RP2 neurons and one sib, rather than the one cell of each type seen in each segment in wild-type embryos. In ago3/Df(3L)GN50 embryos this increases to 7%.
Adult eyes mosaic for ago3 are composed mostly of mutant tissue, exhibiting a proliferative advantage over their wild-type twin-spots. Within mutant clones, most ommatidial clusters lack the wild-type complement of photoreceptor cells, and the distance between adjacent photoreceptor clusters is increased. The large interommatidial spaces in these clones contain excess cells compared to wild-type (from 12 to about 20 per photoreceptor cluster). There is no decrease in the extent of cell death in these clones. When homozygous mutant clones are made in the wing disc, a smaller fraction of cells (21.4%) is found in G1 compared to wild-type cells (36.%). The proportion of cells in S phase is increased. In pupal eye discs cell cycling continues after the corresponding cells in wild-type tissues have exited the cell cycle.
agoΔ3-7/ago3 has lethal phenotype, suppressible | partially by sima[+]/simaKG07607
ago3, slmb00295 has increased cell number | embryonic stage phenotype, suppressible by slmbαTub84B.Tag:MYC
ago3, slmb00295 has increased cell number | embryonic stage phenotype, suppressible by gcmN7-4/gcm[+]
ago3, slmb00295 has increased cell number | embryonic stage phenotype, suppressible by gcm26
ago3 has decreased cell number | somatic clone | adult stage phenotype, suppressible by BacA\p35GMR.PH
ago3, slmb00295 has increased occurrence of cell division | embryonic stage phenotype
ago3, slmb00295 has increased cell number | embryonic stage phenotype
ago3/ago1 has embryonic/larval tracheal dorsal trunk phenotype, enhanceable by Df(2R)Exel6060/+
ago3/ago1 has embryonic/larval tracheal system phenotype, enhanceable by awdj2A4/awd[+]
ago3/ago1 has embryonic/larval tracheal dorsal trunk phenotype, enhanceable by awdj2A4/awd[+]
ago3 has secondary pigment cell | ectopic | somatic clone phenotype, enhanceable by BacA\p35GMR.PH
ago3 has interommatidial bristle | ectopic | somatic clone phenotype, enhanceable by BacA\p35GMR.PH
ago3 has tertiary pigment cell | ectopic | somatic clone phenotype, enhanceable by BacA\p35GMR.PH
agoΔ3-7/ago3 has larval lateral H tracheal branch phenotype, suppressible by sima[+]/simaKG07607
agoΔ3-7/ago3 has tracheole | increased number phenotype, suppressible by sima[+]/simaKG07607
ago3, slmb00295 has glial cell | embryonic stage phenotype, suppressible by slmbαTub84B.Tag:MYC
ago3, slmb00295 has glial cell | embryonic stage phenotype, suppressible by gcmN7-4/gcm[+]
ago3, slmb00295 has glial cell | embryonic stage phenotype, suppressible by gcm26
ago3/ago1 has embryonic/larval dorsal tracheal branch phenotype, suppressible by trh10512/trh[+]
ago3/ago1 has tracheal primordium phenotype, suppressible by trh10512/trh[+]
ago3/ago1 has embryonic/larval ganglionic tracheal branch phenotype, suppressible by trh10512/trh[+]
ago3/ago1 has tracheal fusion cell phenotype, suppressible by trh10512/trh[+]
ago3/ago1 has embryonic/larval tracheal system phenotype, suppressible by btl[+]/btldev1
ago3/ago1 has embryonic/larval tracheal dorsal trunk phenotype, suppressible by btl[+]/btldev1
ago3/ago1 has tracheal lumen phenotype, suppressible by btl[+]/btldev1
ago3/ago1 has embryonic/larval tracheal lateral trunk phenotype, suppressible by btl[+]/btldev1
ago3/ago1 has embryonic/larval dorsal tracheal branch phenotype, suppressible by btl[+]/btldev1
ago3/ago1 has tracheal primordium phenotype, suppressible by btl[+]/btldev1
ago3/ago1 has embryonic/larval ganglionic tracheal branch phenotype, suppressible by btl[+]/btldev1
ago3/ago1 has tracheal fusion cell phenotype, suppressible by btl[+]/btldev1
ago3/ago1 has embryonic/larval tracheal system phenotype, suppressible by trh10512/trh[+]
ago3/ago1 has embryonic/larval tracheal dorsal trunk phenotype, suppressible by trh10512/trh[+]
ago3/ago1 has tracheal lumen phenotype, suppressible by trh10512/trh[+]
ago3/ago1 has embryonic/larval tracheal lateral trunk phenotype, suppressible by trh10512/trh[+]
ago[+]/ago3 is an enhancer of embryonic/larval tracheal section phenotype of Scer\GAL4btl.PS, VhlRNAi.UAS.cMa
ago3, slmb00295 has glial cell | embryonic stage phenotype
Df(2R)Exel6060/+, ago3 has embryonic/larval tracheal section phenotype
simaKG07607 dominantly delays the lethal phase of agoΔ3-7/ago3 transheterozygotes.
simaKG07607 dominantly suppresses the increase in number of terminal branches per lateral LH cell seen in ago3/agoΔ3-7 larvae.
slmb00295, ago3 double mutant embryos display a disrupted glial pattern compared to single mutant or control embryos. An increase in repo-positive glial cell number is observed. An increase in cell proliferation is observed, and no increase in cell death is seen.
Expression of slmbαTub84B.T:Hsap\MYC in slmb00295, ago3 double mutant embryos significantly suppresses the increased number of glial cells observed in the slmb00295, ago3 mutant embryos.
slmb00295, ago3, gcmN7-4 triple mutants results in the absence of almost all glia, as observed in gcmN7-4 single mutants. One copy of gcmN7-4 suppresses the increased glial cell number phenotype observed in slmb00295, ago3 double mutants.
gcm26 suppresses the increased glial cell number phenotype observed in slmb00295, ago3 double mutants.
ago3/+ enhances the tracheal phenotypes seen when Scer\GAL4btl.PS mediates expression of relatively weaker VhldsRNA.Scer\UAS lines, particularly enhancing the penetrance of migration defects.
Embryos trans-heterozygous for ago3 and Df(2R)Exel6060 (that uncovers Vhl) show an elevated frequency of tracheal fusion and migration defects compared to either lesion alone.
Df(2R)Exel6060 (that uncovers Vhl) dominantly enhances the number of dorsal trunk breaks per affected ago3/ago1 embryo.
A trh10512 heterozygous background dominantly suppresses the tracheal phenotypes found in ago1/ago3 mutants.
A btldev1 heterozygous background dominantly suppresses the tracheal phenotypes found in ago1/ago3 mutants.
A awdj2A4 heterozygous background strongly enhances both the penetrance (from 46% of embryos to greater than 80% of embryos) of the ago1/ago3 mutant dorsal trunk phenotype and its expressivity among affected embryos. These embryos also show a much more severe disruption of the entire tracheal system compared to ago1/ago3 mutants.
ago3 does not protect Df(1)su(s)R194/+ clones in the eye; Df(1)su(s)R194/+ ; ago3 clones are not recovered in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals). Also, ago3 does not prevent apoptosis of Df(1)su(s)R194/+ cells in the wing.
The expression of BacA\p35GMR.PH in BacA\p35GMR.PH somatic clones in the eye, results in an increase in the number of interommatidial cells (from about 20 to about 40 per photoreceptor cluster).
The expression of BacA\p35GMR.PH in ago3 somatic clones in the eye, results in an increase in the number of interommatidial cells (from about 20 to about 40 per photoreceptor cluster).
ago3/ago1 is rescued by agoUAS.cMa/Scer\GAL4btl.PS
Expression of agoScer\UAS.cMa in the developing tracheal system under the control of Scer\GAL4btl.PS in an ago1/ago3 mutant background significantly reduces the frequency of dorsal trunk breaks, along with other tracheal phenotypes.