20.5% of fru^w12/fru^P14 embryos have abnormal Fas2-positive fascicles in the central nervous system. The developing medial Fas2-positive fascicle is indistinct in stage 13 embryos and the aCC neuron sometimes either joins the ascending pCC axon or grows posteriorly (instead of extending peripherally as occurs in wild-type embryos). 25.5% of fru^P14/Df(3R)fru^w24 embryos have abnormal Fas2-positive fascicles in the central nervous system. 24.5% of fru^AJ96u3/fru^P14 embryos have abnormal Fas2-positive fascicles and 17.0% have defects in the distribution and pattern of BP102-positive axons in the central nervous system. fru^P14/fru^4-40 embryos have normal Fas2-positive and BP102-positive axon tracts in the central nervous system.

Male-female courtship as measured by courtship index (CI) or wing extension index (WEI) is almost completely abolished in fru^P14/fru^4-40 males. fru^w12/fru^P14 adults that have been dissected from the pupal case show minor external defects, such as uninflated wings, wings held at a 70-90^o angle to the body, leg joint defects and duplicated bristles.