FlyBase Allele Report: Dmel\Antp[UAS.cMb]

General Information

Symbol

Dmel\Antp^UAS.cMb

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Miller

FlyBase ID

FBal0124416

Feature type

allele

Associated gene

Dmel\Antp

Associated Insertion(s)

Carried in Construct

P{UAS-Antp.Mb}

Also Known As

UAS-Antp

Key Links

Transgenic product class

cDNA

(Miller et al., 2001)

wild_type

(Miller et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Miller et al., 2001)

wild_type

(Miller et al., 2001)

Mutagen

in vitro construct

(Miller et al., 2001, Heuer et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-Antp.Mb}

Cytology

Description

UASt sequences drive expression of the entire G1100 cDNA.

(Miller et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Antp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | larval stage, with Scer\GAL4^Antp-10

(Mandal et al., 2007)

lethal, with Scer\GAL4^69B

(Miller et al., 2001)

lethal, with Scer\GAL4^{l(3)31-1-31-1}

(Miller et al., 2001)

lethal, with Scer\GAL4^prd.RG1

(Miller et al., 2001)

lethal - all die before end of P-stage, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

some die during pharate adult stage, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

visible, with Scer\GAL4^dpp.blk1

(Yao et al., 1999)

visible, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

Phenotype Manifest In

adult head, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

antenna, with Scer\GAL4^dpp.blk1

(Yao et al., 1999)

antenna, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

aorta primordium | anterior, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Perrin et al., 2004)

aorta primordium | ectopic | posterior, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

(Perrin et al., 2004)

dorsal acute muscle cell | ectopic, with Scer\GAL4^how-24B

(Enriquez et al., 2010)

embryonic/larval lymph gland, with Scer\GAL4^Antp-10

(Mandal et al., 2007)

embryonic midgut chamber 1, with Scer\GAL4^how-24B

(Miller et al., 2001)

embryonic midgut chamber 2, with Scer\GAL4^how-24B

(Miller et al., 2001)

embryonic midgut constriction 1, with Scer\GAL4^how-24B

(Miller et al., 2001)

embryonic tritocerebrum, with Scer\GAL4^sca-537.4

(Sprecher et al., 2004)

eye, with Scer\GAL4^ey.PH

(Jiao et al., 2001)

gastric caecum primordium, with Scer\GAL4^how-24B

(Miller et al., 2001)

larval thoracic ventral intersegmental muscle cell | ectopic, with Scer\GAL4^how-24B

(Miller et al., 2001)

metathoracic ventral acute muscle cell | ectopic, with Scer\GAL4^how-24B

(Miller et al., 2001)

wing margin, with Scer\GAL4^sd-SG29.1

(Adachi-Yamada et al., 2005)

wing vein, with Scer\GAL4^sd-SG29.1

(Adachi-Yamada et al., 2005)

Detailed Description

Statement

Reference

Scer\GAL4^how-24B-mediated expression of Antp^Scer\UAS.cMb results in an ectopic DA3 muscle in thoracic segment 1. The number of nuclei in this ectopic muscle is identical to that in wild type thoracic segments 2 and 3.

(Enriquez et al., 2010)

Expression of Antp^Scer\UAS.cMb under the control of Scer\GAL4^Antp-10 results in a significant suppression of BrdU incorporation throughout the lymph gland. The zone of differentiated cells is more restricted than in wild-type, being restricted to a thin layer along the distal edge of the lymph gland.

(Mandal et al., 2007)

When Antp^Scer\UAS.cMb is driven by Scer\GAL4^sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.

(Adachi-Yamada et al., 2005)

Stage 16 Antp^Scer\UAS.cMb; Scer\GAL4^how-24B embryos have a normal number of cells in the dorsal vessel.

(Ryan et al., 2005)

Anterior dMP2 neurons do not survive in late embryos expressing Antp^Scer\UAS.cMb under the control of Scer\GAL4^Vap.P0201 (as occurs in wild-type embryos, where these neurons are lost by the late embryonic stage). Expression of Antp^Scer\UAS.cMb under the control of Scer\GAL4^elav-C155 only results in a marginal increase in survival of anterior dMP2 neurons in late embryos compared to wild-type embryos (where these neurons are lost by the late embryonic stage).

(Miguel-Aliaga and Thor, 2004)

In mutant embryos expressing Antp^Scer\UAS.cMb driven by both Scer\GAL4^twi.PG and Scer\GAL4^how-24B ectopic cardioblasts are seen and the lymph glands are systematically eliminated and replaced by major pericardial cells: The anterior aorta is transformed into a posterior aorta and heart tissue. This effect is not seen if Antp^Scer\UAS.cMb is driven by Scer\GAL4^tin.CΔ4.

(Perrin et al., 2004)

Expression of Antp^Scer\UAS.cMb under the control of Scer\GAL4^sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 70-80%.

(Sprecher et al., 2004)

Expression of Antp^Scer\UAS.cMb under the control of Scer\GAL4^lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.

(Hirth et al., 2001)

Flies expressing Antp^Scer\UAS.cMb under the control of Scer\GAL4^ey.PH do not eclose. Three classes of phenotype are seen; class I pharate adults lack all head structures derived from the eye-antennal discs, class II consists of eyeless flies which lack most head structures and both antennae and class III consists of eyeless animals with large parts of the head missing but one or both antennae present.

(Jiao et al., 2001)

When driven by Scer\GAL4^how-24B, some posterior central projections (PVs) are seen in the T1 and T2 segments suggesting T3 identity, and may even display some anterior ventral projection (AV) character in the T2 segment. Animals show an anterior shift of the first midgut constriction with reduced gastric caecum, a reduction of the first midgut chamber and an enlarged second chamber.

(Miller et al., 2001)

When driven by Scer\GAL4^69B, Scer\GAL4^prd.RG1, or Scer\GAL4^{l(3)31-1-31-1} leads to a significant loss in viability.

(Miller et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

Suppressed by

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^ey.PH has lethal | pharate adult stage phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Antp^UAS.cMb, Scer\GAL4^ey.PH has visible phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Antp^UAS.cMb, Scer\GAL4^dpp.blk1 has visible phenotype, suppressible by hth^UAS.cPa, Scer\GAL4^dpp.blk1

(Yao et al., 1999)

Enhancer of

Statement

Reference

cas^UAS.cKa/Antp^UAS.cMb, Scer\GAL4^GMR42G10 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

Suppressor of

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^lz-gal4 is a suppressor of visible phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Anderson et al., 2011)

Other

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^da.G32, cas^UAS.cKa, grh^UAS.cBa has decreased occurrence of cell division | embryonic stage 12 phenotype

(Bahrampour et al., 2017)

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has abnormal neuroanatomy | embryonic stage phenotype

(Bivik et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has thoracic neuroblast | embryonic stage phenotype, non-enhanceable by E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has abdominal neuroblast | embryonic stage phenotype, non-enhanceable by E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

Suppressed by

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^ey.PH has adult head phenotype, suppressible | partially by CycE^UAS.cLa, Scer\GAL4^ey.PH

(Jiao et al., 2001)

Antp^UAS.cMb, Scer\GAL4^dpp.blk1 has antenna phenotype, suppressible by hth^UAS.cPa, Scer\GAL4^dpp.blk1

(Yao et al., 1999)

NOT Enhancer of

Statement

Reference

cas^UAS.cKa/Antp^UAS.cMb, Scer\GAL4^GMR42G10 is a non-enhancer of thoracic neuroblast | embryonic stage phenotype of E(spl)m8-HLH^{CK2.UAS.Tag:FLAG}, Scer\GAL4^GMR42G10

(Bivik et al., 2016)

Suppressor of

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^lz-gal4 is a suppressor of eye phenotype of Scer\GAL4^lz-gal4, sens^UAS.cNa

(Anderson et al., 2011)

Scer\GAL4^lab.PH/Antp^UAS.cMb is a suppressor of embryonic tritocerebrum phenotype of lab¹⁴

(Hirth et al., 2001)

Other

Statement

Reference

Antp^UAS.cMb, Scer\GAL4^da.G32, cas^UAS.cKa, grh^UAS.cBa has embryonic neuroblast | embryonic stage 12 phenotype

(Bahrampour et al., 2017)

Antp^UAS.cMb, Scer\GAL4^da.G32, cas^UAS.cKa, grh^UAS.cBa has embryonic ganglion mother cell | embryonic stage 12 phenotype

(Bahrampour et al., 2017)

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has thoracic neuroblast | embryonic stage phenotype

(Bivik et al., 2016)

Antp^UAS.cMb, Scer\GAL4^GMR42G10, cas^UAS.cKa has abdominal neuroblast | embryonic stage phenotype

(Bivik et al., 2016)

Antp^UAS.cMb, Dfd¹⁶, Scer\GAL4^salm-459.2, Scr⁴ has adult corpus allatum phenotype

(Sánchez-Higueras et al., 2014)

Antp^UAS.cMb, Dfd¹⁶, Scer\GAL4^salm-459.2, Scr⁴ has prothoracic gland phenotype

(Sánchez-Higueras et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The triple co-expression of cas^Scer\UAS.cKa, grh^Scer\UAS.cBa and Antp^Scer\UAS.cMb triggers an apparent reduction in the numbers of dividing neuroblasts and dividing neuroblast daughters in stage 12 embryos, as compared to controls.

(Bahrampour et al., 2017)

Co-expression of cas^Scer\UAS.cKa and Antp^Scer\UAS.cMb under the control of Scer\GAL4^GMR42G10 results in a significant reduction in thoracic neuroblast proliferation at stage 12 (but not 14), neuroblast daughter cell proliferation at stage 14 (but not stage 12), and abdominal neuroblast and neuroblast daughter cell proliferation at both stage 12 and 14, as compared with controls.

Co-expression of E(spl)m8-HLH^{CK2.Scer\UAS.T:Zzzz\FLAG}, cas^Scer\UAS.cKa and Antp^Scer\UAS.cMb under the control of Scer\GAL4^GMR42G10 enhances the reduced proliferation phenotype in neuroblast daughter cells, but does not enhance the reduced proliferation of neuroblasts, as compared with embryos expressing cas^Scer\UAS.cKa and Antp^Scer\UAS.cMb under the control of Scer\GAL4^GMR42G10.

(Bivik et al., 2016)

Addition of Antp^Scer\UAS.cMb to Dfd¹⁶;Scr⁴ double mutant embryos (under the control of Scer\GAL4^salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

(Sánchez-Higueras et al., 2014)

Co-expression of Antp^Scer\UAS.cMb strongly suppresses the disorganised eye phenotype caused by expression of sens^Scer\UAS.cNa under the control of Scer\GAL4^lz-gal4.

(Anderson et al., 2011)

Expression of Antp^Scer\UAS.cMb under the control of Scer\GAL4^lab.PH rescues the tritocerebral defects seen in lab¹⁴ embryonic brains. 34.9% of embryos show a complete rescue of the defects (taking into account that the phenotypic penetrance of the lab¹⁴ phenotype is 88.6%).

(Hirth et al., 2001)

Co-expression of hth^Scer\UAS.cPa partially suppresses the antenna to leg transformation phenotypes produced by ectopic expression of Antp^Scer\UAS.cMb driven by Scer\GAL4^dpp.blk1. The antennae produced are aristaless and occasionally duplicated, similar to the hth^Scer\UAS.cPa phenotype.

(Yao et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7301

w¹¹¹⁸; P{UAS-Antp.Mb}W1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Antp^Scer\UAS.cKa

Antp^Scer\UAS.cMb

Antp^UAS.cKa

Antp^UAS.cMb

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Miller

(Miller et al., 2001)

Secondary FlyBase IDs

FBal0044641

References (32)

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