FlyBase Allele Report: Dmel\arm[043A01]

General Information

Symbol

Dmel\arm^043A01

Species

D. melanogaster

Name

FlyBase ID

FBal0123949

Feature type

allele

Associated gene

Dmel\arm

Associated Insertion(s)

Carried in Construct

Also Known As

arm^O43A01

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tolwinski and Wieschaus, 2001)

Progenitor genotype

Cytology

Description

Nucleotide substitution: ?3404?.

(Tolwinski and Wieschaus, 2004)

Amino acid replacement: ??term.

(Tolwinski and Wieschaus, 2004, Tolwinski and Wieschaus, 2001)

Mutation introduces a premature stop codon prior to repeats 11-12.

(Tolwinski and Wieschaus, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | germline clone

(Tolwinski and Wieschaus, 2004)

Phenotype Manifest In

adherens junction | germline clone

(Tolwinski and Wieschaus, 2004)

embryo | maternal effect

(Mannava and Tolwinski, 2015)

embryonic/first instar larval cuticle | maternal effect

(Kaplan et al., 2009)

embryonic/larval cuticle | embryonic stage

(Sarpal et al., 2012)

ventral furrow | maternal effect

(Martin et al., 2010)

Detailed Description

Statement

Reference

Maternally and zygotically mutant arm^043A01 embryos (coming from mothers whose whole germline constitutes from arm^043A01 mutant cells only - created by the OvoD germline clone technique) fall apart during development due to loss of cell adhesion.

(Mannava and Tolwinski, 2015)

arm^043A01 mutant embryos exhibit cuticle defects.

(Sarpal et al., 2012)

Epithelial tears occur in the ventral furrow of embryos mutant for maternal and zygotic arm^043A01, but tears are not observed in more lateral ectoderm at the same stage. The mutant ventral cells appear to round up upon loss of adhesion. Despite the dramatic loss of adhesion, cells in maternal and zygotic arm^043A01 mutant embryos maintain thin membrane connections, or tethers, to their neighbours.

(Martin et al., 2010)

Cuticle fails to form in embryos mutant for arm^043A01 both maternally and zygotically.

(Kaplan et al., 2009)

arm^043A01 clones deficient for both maternal and zygotic arm display gaps between furrows of cellularization embryos. Cell-cell adhesion is compromised and no detectable basal junctions form. However, furrows form and ingress around all nuclei in these embryos as in wild-type. These furrow canals contract during late cellularization when the basal surface of the cell is normally closed-off from the yolk mass. Furrow canals are discrete tear-shaped compartments. Furrow tips broaden to form distinct furrow canal structures in these embryos.

(Sokac and Wieschaus, 2008)

When homozygous mutant germ-line clones are made, mutant embryos disintegrate, as cell delaminate owing to an inability to form adherens junctions.

(Tolwinski and Wieschaus, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

arm^043A01 has embryo | maternal effect phenotype, suppressible by sgg^{UAS.Tag:Myr(Src64B),Tag:HA}/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

arm^043A01 has embryonic/larval cuticle | embryonic stage phenotype, suppressible by SCAR^Δ37/SCAR[+]

(Sarpal et al., 2012)

arm^043A01 has embryonic/larval cuticle | embryonic stage phenotype, suppressible by Arp3^EP3640/Arp3[+]

(Sarpal et al., 2012)

NOT suppressed by

Statement

Reference

arm^043A01 has embryo | maternal effect phenotype, non-suppressible by sgg^UAS.Tag:HA/Scer\GAL4^arm.PU

(Mannava and Tolwinski, 2015)

Other

Statement

Reference

arm^043A01, l(2)gl⁴ has embryonic/first instar larval cuticle | maternal effect phenotype

(Kaplan et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^arm.PU-driven expression of sgg^{Scer\UAS.T:Myr-Src64B,T:Ivir\HA1} (but not sgg^{Scer\UAS.T:Ivir\HA1}) can mildly rescue cuticle integrity in arm^043A01 maternally and zygotically mutant arm^043A01 embryos.

(Mannava and Tolwinski, 2015)

One copy of SCAR^Δ37 partially suppresses the cuticle defects seen in arm^043A01 mutant embryos.

One copy of Arp3^EP3640 partially suppresses the cuticle defects seen in arm^043A01 mutant embryos.

(Sarpal et al., 2012)

Embryos double mutant for arm^043A01 both maternally and zygotically and l(2)gl⁴ zygotically display abnormal embryonic cuticle.

(Kaplan et al., 2009)

Expression of nullo^{Scer\UAS.T:Ivir\HA1}, under the regulation of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} in an arm^043A01 mutant, results in cells that are unable to flatten or constrict their apical surfaces as in nullo^{Scer\UAS.T:Ivir\HA1} (Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}) single mutants.

(Dawes-Hoang et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

arm^043A01 is rescued by arm^{S10.UAS.Tag:MYC}/Scer\GAL4^arm.PS

(Tolwinski and Wieschaus, 2001)

Partially rescued by

arm^043A01 is partially rescued by arm^{Tag:MYC,Tag:PM(hKRAS)}

(Tolwinski and Wieschaus, 2004)

Not rescued by

arm^043A01 is not rescued by arm^{ΔN.UAS.Tag:HA,Tag:Myr(Unk)}/Scer\GAL4^arm.PS

(Tolwinski and Wieschaus, 2001)

Comments

The adhesion defect of arm^043A01 is not rescued by expression of arm^{Δ.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^arm.PS. The adhesion defect of arm^043A01 is rescued by expression of arm^{S10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^arm.PS.

(Tolwinski and Wieschaus, 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

arm^0403A1

(Sarpal et al., 2012)

arm^043A01

(Chanet et al., 2017, Mannava and Tolwinski, 2015, Colosimo et al., 2010, Martin et al., 2010, Kaplan et al., 2009, Sawyer et al., 2009, Sokac and Wieschaus, 2008, Mosimann et al., 2006)

arm^O43A01

(Colosimo et al., 2010, Kaplan et al., 2009, Blankenship et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (15)

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