The insertion site of P{lacW}Pknk06808 is within the first intron of Pkn. The orientation of the inserted element is identical to the direction of the transcribed strand of Pkn.
Flies homozygous for Pknk06808 are unable to fly and their wings are held up from the thorax and curve ventrally, similar to the open doors of a Delorean automobile. The extent of curvature is variable, from slight smooth curve to severe folded-over phenotype. The anterior wing margin is also defective: there are gaps in marginal tissue, excessive spacing between stout mechanosensory bristles, and fewer sensillae and bristles relative to heterozygous siblings. Occasionally, two or three stout bristles share a common socket, and sometimes a stout bristle appears in the margin tissue without a socket. A total of 95% of homozygous Pknk06808 flies analysed have one or more double-stout bristle clusters, and 15% have triple-stout bristle clusters.
Wing defects are only seen in homozyogus Pknk06808 flies. Heterozygous Pknk06808/+ flies have wild-type wings.
Wing vein defects, such as ectopic vein material surrounding the longitudinal vein L2, and/or extending from the posterior crossvein, are also noted.
Pknk06808/Pknk06808; Tp(2;3)eve1.18 wings do not exhibit ventral curvature and flies no longer have ante- rior wing margin bristle defects or ectopic veins associated with the delorean phenotype.
When homozygous Pknk06808 offspring are compared with heterozygous Pknk06808/+ siblings, no significant difference in viability is observed. Thus, the Pknk06808 allele is adult viable.
Unexpectedly, wing shape, anterior wing margin structures, and venation patterns of Pknk06808/Df(2R)w45-30n flies are mostly wild-type. This was seen for all deficiencies of 45C examined (Df(2R)Np3, Df(2R)Np5, and Df(2R)wun-GL).
Homozygotes have wings that stand up from the thorax and curve down. The phenotype also manifests itself in mechanosensory bristles of the anterior wing margins.
Pknk06808 has wing margin bristle phenotype, enhanceable by bsk1/bsk[+]
Pknk06808 has anterior wing margin phenotype, enhanceable by nmoP1/nmo[+]
Pknk06808 has wing phenotype, non-enhanceable by LIMK1EY08757
Pknk06808 has wing phenotype, non-suppressible by LIMK1EY08757
Etymology: mutation is named "delorean" as mutant flies have wings that stand up from the thorax and curve down, much like the open doors of a Delorean car.