FlyBase Allele Report: Dmel\cher[EP3715]

General Information

Symbol

Dmel\cher^EP3715

Species

D. melanogaster

Name

FlyBase ID

FBal0121831

Feature type

allele

Associated gene

Dmel\cher

Associated Insertion(s)

P{EP}cher^EP3715

Carried in Construct

Also Known As

EP(3)3715

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Sokol and Cooley, 2003, Gene Disruption Project members, 2001-, Sokol and Cooley, 2001, Guo et al., 2000, Li et al., 1999)

Progenitor genotype

Associated Insertion(s)

P{EP}cher^EP3715

Cytology

Description

The orientation of the P{EP} element is the same as the direction of cher transcription.

(Shulman and Feany, 2003)

P{EP} insertion in the 5' regulatory region of cher. The P{EP} element is oriented in the same direction as the direction of transcription of cher.

(Guo et al., 2000)

P{EP} insertion 78bp upstream of the 5' end of the cher EST GH12209.

(Li et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:17,119,140..17,119,140 [-]

(Lawlor et al., 2011, Shulman and Feany, 2003, Sokol and Cooley, 2003, Gene Disruption Project members, 2001-, Sokol and Cooley, 2001, Guo et al., 2000, Li et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Shulman and Feany, 2003)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Sokol and Cooley, 2003)

Phenotype Manifest In

egg chamber

(Sokol and Cooley, 2003)

follicle cell

(Sokol and Cooley, 2003)

germarium

(Sokol and Cooley, 2003)

ovariole

(Sokol and Cooley, 2003)

ovary

(Sokol and Cooley, 2003)

stalk follicle cell

(Sokol and Cooley, 2003)

Detailed Description

Statement

Reference

Ovaries from cher^EP3715 females are dramatically reduced in size. and are comprised of short, squat ovarioles that usually appear as a single, distended germarium from which egg chambers fail to emerge. Closer examination these germaria shows that consecutive germline cysts are not properly packaged in follicular monolayers to form discrete egg chambers. In germaria from cher^EP3715 homozygous adults, individual sixteen-cell germline cysts appear to form normally and the fusome disappears on schedule. Follicle cells extend projections to surround the germline cells. However, these extensions appear to be delayed since there are many more germline cells between the anterior tip of the germarium and where these extensions form than in wild-type. Migration of follicle cells to form the interfollicular stalk fails to occur. In fact these cells fail to even make the apical contacts that precede this migration. However, follicle cell apicobasal polarity is not directly affected. In addition, germline cells appear loosely packed and individual germ-cells often bulge out from a cyst, sometimes making contact with the basal membrane of the germarium.

(Sokol and Cooley, 2003)

The P{EP}cher^EP3715 insertion does not disrupt cher function.

(Li et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

cher^EP3715, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

NOT Enhancer of

Statement

Reference

cher^EP3715, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

cher^EP3715, Scer\GAL4^pnr-MD237 is a suppressor of visible phenotype of Psn^UAS.cGa, Scer\GAL4^pnr-MD237

(Guo et al., 2000)

cher^EP3715, Scer\GAL4^ct.PB is a suppressor of visible phenotype of Psn^UAS.cGa, Scer\GAL4^ct.PB

(Guo et al., 2000)

NOT Suppressor of

Statement

Reference

cher^EP3715, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

cher^EP3715, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

cher^EP3715, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

NOT Enhancer of

Statement

Reference

cher^EP3715, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

cher^EP3715, Scer\GAL4^pnr-MD237 is a suppressor of scutellar bristle | increased number phenotype of Psn^UAS.cGa, Scer\GAL4^pnr-MD237

(Guo et al., 2000)

cher^EP3715, Scer\GAL4^ct.PB is a suppressor of wing phenotype of Psn^UAS.cGa, Scer\GAL4^ct.PB

(Guo et al., 2000)

NOT Suppressor of

Statement

Reference

cher^EP3715, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN1^82.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The notched wing phenotype caused by expression of Psn^Scer\UAS.cGa under the control of Scer\GAL4^ct.PB is suppressed by co-expression of cher^EP3715 (under the control of Scer\GAL4^ct.PB). The extra scutellar macrochaetae seen when Psn^Scer\UAS.cGa is expressed under the control of Scer\GAL4^pnr-MD237 are suppressed by co-expression of cher^EP3715 (under the control of Scer\GAL4^pnr-MD237).

(Guo et al., 2000)

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by cher^EP3715.

(Shulman and Feany, 2003)

Complementation and Rescue Data

Complements

cher^Q1042X

(Sokol and Cooley, 2003)

cher^Q683X

(Sokol and Cooley, 2003)

cher^sko

(Sokol and Cooley, 2003)

Fails to complement

cher^EPSΔ5

(Sokol and Cooley, 2003)

Rescued by

cher^EP3715 is rescued by Scer\GAL4^e22c, cher^EP3715

(Sokol and Cooley, 2003)

Partially rescued by

cher^EP3715 is partially rescued by cher^1-6.hsp70

(Sokol and Cooley, 2003)

cher^EP3715 is partially rescued by cher^hsp70.PS

(Sokol and Cooley, 2003)

Rescues

Scer\GAL4^e22c, cher^EP3715 rescues cher^EP3715

(Sokol and Cooley, 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Precise excision of the P{EP} element reverts the modifying effect of cher^EP3715 on the rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Shulman and Feany, 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

EP(3)3715

(Sokol and Cooley, 2001, Guo et al., 2000, Li et al., 1999)

cher^EP(3)3175

(Sokol and Cooley, 2003)

cher^EP(3)3715

(Lawlor et al., 2011)

cher^EP3175

cher^EP3715

Name Synonyms

Secondary FlyBase IDs

FBal0101478

References (7)

Report Sections

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