FB2024_03 , released June 25, 2024
Allele: Dmel\dppUAS.T.EGFP
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General Information
Symbol
Dmel\dppUAS.T.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0120515
Feature type
allele
Associated gene
Dmel\dpp
Associated Insertion(s)
Carried in Construct
P{UAS-dpp.GFP.T}
Also Known As
UAS-Dpp-GFP, UAS-Dpp::GFP, uas-Dpp
Key Links
Transgenic product class
cDNA
(Teleman and Cohen, 2000)
wild_type
(Teleman and Cohen, 2000)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Teleman and Cohen, 2000)
wild_type
(Teleman and Cohen, 2000)
Mutagen
in vitro construct
(Teleman and Cohen, 2000)
Progenitor genotype
Carried in construct
P{UAS-dpp.GFP.T}
Cytology
Description

UASt regulatory sequences drive expression of dpp cDNA e5 that is tagged downstream of the predicted proteolytic cleavage site with EGFP.

(Teleman and Cohen, 2000)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dpp
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The adulthood-only expression of dppScer\UAS.T.T:Avic\GFP-EGFP (using Gal80[ts] for the temporal regulation of expression) under the control of either Scer\GAL4esg.PU or Scer\GAL4Acp26Aa.PC leads to a significant increase in the size of secondary cell nuclei, but not of main cell nuclei, as compared to controls; the Scer\GAL4esg.PU-induced phenotype is cell autonomous.

      (Redhai et al., 2016)

      Overexpression of dppScer\UAS.T.T:Avic\GFP-EGFP in AB cells using Scer\GAL4ptc-559.1 casues an increase in the size of the posterior compartment. Overexpression under the control of Scer\GAL4bi-omb-Gal4, however, results in a reduction in the differences between anterior and posterior compartments in the haltere discs.

      (Makhijani et al., 2007)

      Expression of dppScer\UAS.T.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.blk1 results in imaginal disc overgrowth.

      (Teleman and Cohen, 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      NOT Enhanced by
      NOT suppressed by
      Phenotype Manifest In
      Enhanced by
      NOT Enhanced by
      NOT suppressed by
      Suppressor of
      Statement
      Reference

      dppUAS.T.EGFP/Scer\GAL4dpp.blk1 is a suppressor of dorsal mesothoracic disc & cytoskeleton phenotype of coroex6/coroex11

      (Bharathi et al., 2004)

      dppUAS.T.EGFP/Scer\GAL4dpp.blk1 is a suppressor of dorsal mesothoracic disc & cytoskeleton phenotype of coroex6

      (Bharathi et al., 2004)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Discs overexpressing dppScer\UAS.T.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.blk1 in coroex11 or coroex6 homozygous or heterozygous mutants or coroex6/coroex11 transheterozygous mutants are much larger than wild-type discs expressing dppScer\UAS.T.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.blk1. In coroex6 homozygous mutants and coroex6/coroex11 mutants, a partial rescue of the actin cytoskeleton and apicobasal polarity is seen in wing discs overexpressing dppScer\UAS.T.T:Avic\GFP-EGFP under the control of Scer\GAL4dpp.blk1.

      (Bharathi et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      dppUAS.T.EGFP/Scer\GAL4dpp.blk1 partially rescues dppd12/dppd8

      (Teleman and Cohen, 2000)
      Comments

      dppScer\UAS.T.T:Avic\GFP-EGFP rescues the wing phenotype of dppd8/dppd12 flies when expressed under the control of Scer\GAL4dpp.blk1; wing size and pattern are almost normal except that ectopic vein material is seen near vein 3 and the spacing between veins 3 and 4 is slightly increased.

      (Teleman and Cohen, 2000)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      dppScer\UAS.T.T:Avic\GFP-EGFP
      dppScer\UAS.T.T:Avic\GFP-F64L.S65T
      dppUAS.T.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (18)