FlyBase Allele Report: Dmel\acj6[1,4.UAS]

General Information

Symbol

Dmel\acj6^1,4.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0117612

Feature type

allele

Associated gene

Dmel\acj6

Associated Insertion(s)

Carried in Construct

P{UAS-acj6.1,4}

Also Known As

UAS-acj6(1,4)

Key Links

Transgenic product class

wild_type

(Certel et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Certel et al., 2000)

Mutagen

in vitro construct

(Certel et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-acj6.1,4}

Cytology

Description

This construct contains exons 1, 4, the long form of exon 5, 6, 7, the short form of exon 8, and exon 9.

(Bai and Carlson, 2010)

UASt regulatory sequences drive expression of the acj6 "1,4" isoform.

(Certel et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

acj6

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^GH146

(Komiyama et al., 2003)

lethal | larval stage, with Scer\GAL4^ChAT.7.4

(Lee and Salvaterra, 2002)

lethal | pupal stage, with Scer\GAL4^ChAT.7.4

(Lee and Salvaterra, 2002)

Phenotype Manifest In

antennal glomerulus & neuron, with Scer\GAL4^GH146

(Komiyama et al., 2003)

motor neuron | embryonic stage, with Scer\GAL4^sca-537.4

(Certel et al., 2000)

olfactory sensory organ & neuron & dendrite, with Scer\GAL4^GH146

(Komiyama et al., 2003)

Detailed Description

Statement

Reference

Expression of acj6^1,4.Scer\UAS under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background is able to induce a fenchone odor response, indicating activation of Or85e.

(Bai and Carlson, 2010)

When acj6^1,4.Scer\UAS is driven by Scer\GAL4^GH146 in the longitudinal projection neurons (lPN) three phenotypes are seen. Reduces innervation of appropriate glomeruli, non-specific accumulation and specific mistargeting to inappropriate glomeruli. lPN misexpressing acj6^1,4.Scer\UAS still innervate the same 10 lateral landmark glomeruli (DA1, DL3, DA2, DM5, DM2, DM1, VA7m,VA5, VA4 and VC2) as wild-type, though they occasionally innervate some glomeruli more weakly or partially. Secondly they exhibit diffuse dendritic projections that span part of the lateral side of the antennal lobe with associated glomerular abnormalities. Thirdly mutant lPNs also mistarget their dendrites to well developed landmark glomeruli that are normal reserved for anterodorsal projection neurons (adPNs). This mistargeting often leads to only partial occupation of these glomeruli. It appears to be a specific effect, since glomeruli with mistargeted dendrites are frequently seen surrounded by uninnervated glomeruli.

(Komiyama et al., 2003)

Expression of acj6^1,4.Scer\UAS under the regulation of Scer\GAL4^Cha.7.4 results in third instar larval or late pupal stage lethality.

(Lee and Salvaterra, 2002)

Expression of acj6^1,4.Scer\UAS under the control of Scer\GAL4^sca-537.4 results in a failure of motor axons of the ISNb fascicle to defasciculate from the ISN fascicle, resulting in defective innervation of the ventrolateral muscle field in approximately 65% of embryonic hemisegments. In approximately 15% of hemisegments, the ISNb motor axons stop at the correct location of their target muscles, but do not branch out to innervate the ventral muscle groups. The remaining 20% of hemisegments show a wild-type muscle innervation pattern.

(Certel et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

acj6^1,4.UAS/Scer\GAL4^GH146 is a suppressor | partially of abnormal neuroanatomy | somatic clone | adult stage phenotype of ems³

(Lichtneckert et al., 2008)

Phenotype Manifest In

Suppressor of

Statement

Reference

acj6^1,4.UAS/Scer\GAL4^GH146 is a suppressor of adult antennal lobe projection neuron VA1v adPN | somatic clone phenotype of ems³

(Lichtneckert et al., 2008)

NOT Suppressor of

Statement

Reference

acj6^1,4.UAS/Scer\GAL4^GH146 is a non-suppressor of adult antennal lobe projection neuron VA3 adPN | somatic clone phenotype of ems³

(Lichtneckert et al., 2008)

acj6^1,4.UAS/Scer\GAL4^GH146 is a non-suppressor of adult antennal lobe projection neuron VM2 adPN | somatic clone phenotype of ems³

(Lichtneckert et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of acj6^1,4.Scer\UAS under the control of Scer\GAL4^GH146 in homozygous ems³ adPN neuroblast clones (induced in the early larva and analysed in the adult) significantly rescues the innervation defects of the VA1lm glomerulus, but does not significantly rescue the innervation defects of the VA3 and VM2 glomeruli which are seen in single mutant homozygous ems³ adPN neuroblast clones.

(Lichtneckert et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

acj6^1,4.UAS/Scer\GAL4^GH146 rescues acj6⁶

(Komiyama et al., 2003)

Partially rescues

Scer\GAL4^acj6-PG63/acj6^1,4.UAS partially rescues acj6^PG63

(Bai and Carlson, 2010)

Scer\GAL4^acj6-PG63/acj6^1,4.UAS partially rescues acj6⁶

(Bai and Carlson, 2010)

Comments

Expression of acj6^1,4.Scer\UAS under the control of Scer\GAL4^acj6-PG63 rescues the response of the sensilla pb1A neuron to ethyl acetate, isoamyl acetate, 4-methyl phenol, benzaldehyde and fenchone.

Expression of acj6^1,4.Scer\UAS under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background is able to induce a fenchone odor response, indicating activation of Or85e.

Expression of acj6^1,4.Scer\UAS under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background suppresses the (pb2C, 0) type of sensilla normally found in acj6^PG63 mutants.

Expression of acj6^1,4.Scer\UASunder the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background induces two unexpected types of sensilla. One type contains a normal pb2B cell and a novel ORN that responds to 3-octanol but to no other odorants. This ORN is termed here pb2D. This (pb2D, pb2B) sensillum represents 25 and 21% of the sensilla containing pb2A or pb2B neurons in flies expressing acj6^1,4.Scer\UAS and acj6^{1,3,4.Scer\UAS}, respectively. The second type of sensilla, representing 45% of the pb2 sensilla in acj6^1,4.Scer\UAS expressing flies, respectively, contains a normal pb2B cell and a novel ORN that responds to both 3-octanol and fenchone and is termed her pb2E.

Expression of acj6^1,4.Scer\UAS under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background, fully rescues the sensilla pb1 phenotype, yielding (pb1A, pb1B) sensilla that respond as expected to all odorants tested (ethyl acetate, isoamyl acetate, 4-methyl phenol, benzaldehyde, 3-octanol and 4-propyl phenol).

Expression of acj6^1,4.Scer\UAS under the control of Scer\GAL4^acj6-PG63 in a acj6^PG63/Y background rescues the pb3 sensilla.

(Bai and Carlson, 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

acj6^1,4.Scer\UAS

acj6^1,4.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

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