FlyBase Allele Report: Dmel\Pten[1]

General Information

Symbol

Dmel\Pten¹

Species

D. melanogaster

Name

FlyBase ID

FBal0104064

Feature type

allele

Associated gene

Dmel\Pten

Associated Insertion(s)

Carried in Construct

Also Known As

DPTEN¹

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Goberdhan et al., 1999)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

crossvein | somatic clone

(Goberdhan et al., 1999)

epithelial cell | pupal stage | somatic clone

(Bardet et al., 2013)

eye (with Pten⁵)

(Mensah et al., 2015)

eye | somatic clone

(Goberdhan and Wilson, 2002, Goberdhan et al., 1999)

eye disc | somatic clone

(Bateman and McNeill, 2004)

eye photoreceptor cell | somatic clone

(Pinal et al., 2006)

indirect flight muscle cell (with Pten⁵)

(Mensah et al., 2015)

interommatidial bristle | somatic clone

(Goberdhan et al., 1999)

larval dorsal multidendritic neuron ddaC | pupal stage | somatic clone

(Wong et al., 2013)

lipid particle & nurse cell | somatic clone

(Vereshchagina and Wilson, 2006)

mitochondrion (with Pten⁵)

(Mensah et al., 2015)

ommatidial precursor cluster | somatic clone

(Bateman and McNeill, 2004)

ommatidium | somatic clone

(Pinal et al., 2006, Bateman and McNeill, 2004, Goberdhan and Wilson, 2002, Goberdhan et al., 1999)

photoreceptor neuron | cell autonomous | somatic clone

(Goberdhan et al., 1999)

rhabdomere | cell autonomous | somatic clone

(Goberdhan et al., 1999)

rhabdomere | somatic clone

(Pinal et al., 2006)

wing (with Pten⁵)

(Mensah et al., 2015)

wing & cell | somatic clone

(Goberdhan et al., 1999)

wing | pupal stage | somatic clone

(Bardet et al., 2013)

wing | somatic clone

(Goberdhan et al., 1999)

wing hair | ectopic | somatic clone

(Goberdhan et al., 1999)

Detailed Description

Statement

Reference

Pten⁵/Pten¹ flies exhibit a highly penetrant, but mild, disorganised eye phenotype at the posterior edge in the midline.

Transheterozygous mutant Pten⁵/Pten¹ males have significantly (~20%) greater body mass than wild-type.

A small proportion of newly eclosed Pten⁵/Pten¹ flies display outstretched and paralysed wing phenotype. The flies are unable to fly. The proportion of flightless mutant increases as flies age.

(Mensah et al., 2015)

The integrity of the follicle cell epithelium is not compromised in egg chambers containing homozygous follicle cell clones.

(Claret et al., 2014)

Somatic clones of homozygous Pten¹ mutant future-intervein cells display disrupted hexagonal packaging. In the mutant tissue, only 50% of the cells are six-sided. Furthermore, the tissue exhibits cell patterns with distinctive local cobblestone patterns and some rosette patterns. The cobblestone pattern refers to hexagons with two sides much shorten than the others.

(Bardet et al., 2013)

Pten¹ mutant ddaC neuron clones exhibit dendrite pruning defects at 16 hours after puparium formation.

(Wong et al., 2013)

Pten¹ mutant somatic clones generated in the eye display highly disorganised ommatidia with compromised apical membranes. There is a failure of rhabdomere morphogenesis, and most photoreceptors display abnormally elongated, deformed, or split rhabdomeres. These defects are evident from mid-pupal development onwards. There is no evidence of photoreceptor degeneration in retinas from a series of aged adult flies.

(Pinal et al., 2006)

Nurse cells in 90% of Akt1¹ mutant clones show cell-autonomous formation of large aggregated droplets and a corresponding reduction in small perinuclear droplets compared to wild-type nurse cells.

(Vereshchagina and Wilson, 2006)

Mutant clones in the adult eye produce large cells and misrotated ommatidia. Cells in mutant clones in the eye disc show precocious differentiation several rows ahead of wild-type cells. In addition, ommatidia within clones in the eye disc initiate rotation before wild-type ommatidia.

(Bateman and McNeill, 2004)

Mosaic animals in which the eye is largely homozygous for Pten¹ (generated using the "eyFLP" system) have larger eyes than normal. The eyes contain about 12% more ommatidia than wild-type eyes and individual ommatidia are 78% larger in surface area.

(Goberdhan and Wilson, 2002)

Hemizygotes die as late embryos or early first instar larvae. Homozygous mutant clones in the eye bulge out from the eye surface. Ommatidia sometimes fuse interommatidial bristles are abnormal, particularly (but not exclusively) in the dorsal half of the eye. Mutant clones typically contain at least twice as many ommatidia as their wild type twin spots. The largest clones can form a hyperplastic tumor-like overgrowth, though this phenotype is less severe than for tumor suppressor mutations. Within each ommatidium cell type specification seems normal. Cell bodies of mutant photoreceptors are enlarged compared to wild type neighbors, and the rhabdomeres are misshapen. The cell size and rhabdomeric phenotypes are cell autonomous. Homozygous mutant clones in the wing reveal an increase in cell size. Wing hairs are occasionally duplicated and crossvein formation is abnormal. Occasional extra wing vein material develops. Longitudinal veins are rarely affected.

(Goberdhan et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pten¹/Pten⁵ has flightless | progressive phenotype, suppressible | partially by Buffy^UAS.cQa/Scer\GAL4^arm.PS

(Mensah et al., 2015)

Pten¹/Pten⁵ has flightless | progressive phenotype, suppressible | partially by Buffy^UAS.cQa/Scer\GAL4^how-24B

(Mensah et al., 2015)

Pten¹/Pten⁵ has flightless | progressive phenotype, suppressible | partially by Rheb[+]/Rheb^AV4

(Mensah et al., 2015)

Pten¹/Pten⁵ has flightless | progressive phenotype, suppressible | partially by Tor[+]/mTor^ΔP

(Mensah et al., 2015)

NOT suppressed by

Statement

Reference

Pten¹ has increased body size | recessive | somatic clone phenotype, non-suppressible by chico^flp147E/chico^flp147E

(Goberdhan and Wilson, 2002)

NOT Suppressor of

Statement

Reference

Pten¹/Pten[+] is a non-suppressor of increased body size phenotype of Scer\GAL4^29BD, dally^{sec.UAS.Tag:MYC}

(Takeo et al., 2005)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Pten¹ has eye | somatic clone phenotype, non-suppressible by chico^flp147E/chico^flp147E

(Goberdhan and Wilson, 2002)

Suppressor of

Statement

Reference

Pten¹ is a suppressor | partially of wing phenotype of Pi3K92E^{D954A.UAS.Tag:MYC}, Scer\GAL4^Bx-MS1096

(Goberdhan et al., 1999)

NOT Suppressor of

Statement

Reference

Pten¹/Pten[+] is a non-suppressor of pupa phenotype of Scer\GAL4^29BD, dally^{sec.UAS.Tag:MYC}

(Takeo et al., 2005)

Pten¹/Pten[+] is a non-suppressor of wing phenotype of Scer\GAL4^29BD, dally^{sec.UAS.Tag:MYC}

(Takeo et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Pten¹/+; dally^{sec.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^29BD pupae show the same large pupae and disordered wing patterning phenotype as wild-type animals expressing dally^{sec.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^29BD.

(Takeo et al., 2005)

Mosaic animals in which the eye is largely homozygous for both Pten¹ and chico^flp147E (generated using the "eyFLP" system) have greatly enlarged eyes. The eyes contain about the same number of ommatidia as wild type, but the ommatidia are larger in surface area than normal.

(Goberdhan and Wilson, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Pten³

(Goberdhan et al., 1999)

Rescued by

Pten¹ is rescued by Pten^2.UAS.EGFP/Scer\GAL4^GMR.PF

(Pinal et al., 2006)

Pten¹ is rescued by Pten^+t6.3

(Goberdhan et al., 1999)

Pten¹ is rescued by Pten^hs.PG

(Goberdhan et al., 1999)

Partially rescued by

Pten¹/Pten⁵ is partially rescued by Pten^+t6

(Mensah et al., 2015)

Pten¹ is partially rescued by Pten^3.UAS.EGFP/Scer\GAL4^GMR.PF

(Pinal et al., 2006)

Comments

Expression of Pten^{2.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PF is able to suppress all rhabdomeric phenotypes in Pten¹ mutant eyes.

Expression of Pten^{3.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PF is able to suppress most of the mutant Pten¹ eye phenotype, but some split and misshapen rhabdomeres are still evident.

(Pinal et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DPTEN¹

(Lu et al., 2004, Goberdhan and Wilson, 2002, Goberdhan et al., 1999)

PTEN¹

(Wong et al., 2013, Ogmundsdóttir et al., 2012, Vereshchagina et al., 2008, Betschinger et al., 2006, Pinal et al., 2006)

Pten¹

(Mensah et al., 2015, Vereshchagina and Wilson, 2006)

pten¹

(Claret et al., 2014, Claret et al., 2014, Bardet et al., 2013, Bateman and McNeill, 2004)

Name Synonyms

Secondary FlyBase IDs

References (15)

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