FlyBase Allele Report: Dmel\Mhc[L1736P]

General Information

Symbol

Dmel\Mhc^L1736P

Species

D. melanogaster

Name

FlyBase ID

FBal0101378

Feature type

allele

Associated gene

Dmel\Mhc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Nongthomba and Ramachandra, 1999)

Progenitor genotype

Cytology

Description

Amino acid replacement: L1736P.

(Salvi et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:16,786,216..16,786,216 [+]

Nucleotide change:

T16786216C

Amino acid change:

Reported amino acid change:

L1736P

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Salvi et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless

(Salvi et al., 2012)

flightless (with Mhc^E1570K)

(Salvi et al., 2012)

flightless | recessive

(Nongthomba and Ramachandra, 1999)

sterile (with Mhc¹)

(Nongthomba and Ramachandra, 1999)

sterile | recessive

(Nongthomba and Ramachandra, 1999)

viable

(Nongthomba and Ramachandra, 1999)

visible | recessive

(Nongthomba and Ramachandra, 1999)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell

(Salvi et al., 2012, Nongthomba and Ramachandra, 1999)

indirect flight muscle & myofibril

(Nongthomba and Ramachandra, 1999)

indirect flight muscle cell

(Nongthomba and Ramachandra, 1999)

indirect flight muscle cell (with Mhc¹)

(Nongthomba and Ramachandra, 1999)

indirect flight muscle cell (with Mhc⁷)

(Nongthomba and Ramachandra, 1999)

indirect flight muscle cell (with Mhc¹³)

(Nongthomba and Ramachandra, 1999)

sarcomere | adult stage

(Salvi et al., 2012)

wing

(Nongthomba and Ramachandra, 1999)

Z disc | adult stage

(Salvi et al., 2012)

Detailed Description

Statement

Reference

Homozygous Mhc^L1736P mutant dorsal longitudinal muscles (DLM) show fibre thinning only at the anterior ends. Reduced birefringence is also present. Homozygous Mhc^L1736P dorsal longitudinal muscles show a severe pattern of sarcomeric disarray and contain some larger, punctate accumulations of Z-disc material. Small, clustered bundles of thick/thin filament lattice are seen. The Z discs have little regularity in their size and spacing and the I-bands, H-bands and M bands are not present. No DLM muscle defects are seen in heterozygotes. Z discs of an irregular shape and size are first detectable at 42-46h after puparium formation.

The dorsal longitudinal muscles of Mhc^E1570K/Mhc^L1736P transheterozygotes show fibre thinning at both the anterior and posterior ends. The flies are flightless.

(Salvi et al., 2012)

Mhc^L1736P interacts with Mhc¹, Mhc⁷ or Mhc¹³ in trans to give muscle phenotypes that are intermediate between the two or show severe indirect flight muscle degeneration.

(Nongthomba and Ramachandra, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Mhc^L1736P is rescued by Mhc^+t41.9

(Salvi et al., 2012)

Comments

Expression of Mhc^+t41.9 rescues the dorsal longitudinal muscle defects seen in Mhc^L1736P mutant flies. The flightlessness is not rescued.

(Salvi et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mhc^L1736P

(Salvi et al., 2012)

Mhc^RU2

(Salvi et al., 2012)

ifm(2)RU2

(Salvi et al., 2012)

ifm(2)RU2¹

(Babu and Ramachandra, 2004)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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