FlyBase Allele Report: Dmel\Sxl[N.Hsp83.lacZ]

General Information

Symbol

Dmel\Sxl^N.Hsp83.lacZ

Species

D. melanogaster

Name

FlyBase ID

FBal0098289

Feature type

allele

Associated gene

Dmel\Sxl

Associated Insertion(s)

Carried in Construct

P{Hsp83-Sxl-lacZ.N}

Key Links

Transgenic product class

dominant_negative_variant

(Deshpande et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Deshpande et al., 1999)

Mutagen

in vitro construct

(Deshpande et al., 1999)

Progenitor genotype

Carried in construct

P{Hsp83-Sxl-lacZ.N}

Cytology

Description

An Hsp83 promoter drives expression of a chimeric protein consisting of Sxl amino acids 1 to 99 fused to lacZ. The protein acts as a dominant negative.

(Deshpande et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp83

Encoded product / tool

Sxl

Tagged with

lacZ

reporter enzyme

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color | male

(Deshpande et al., 1999)

abnormal sex-determination

(Deshpande et al., 1999)

lethal | female | heat sensitive

(Deshpande et al., 1999)

partially lethal | dominant | female

(Penn et al., 2008)

viable, with Sxl^fP7B0

(Kappes et al., 2011)

visible | male

(Deshpande et al., 1999)

Phenotype Manifest In

abdominal sternite bristle | male

(Deshpande et al., 1999)

adult abdomen | male

(Deshpande et al., 1999)

male genitalia

(Deshpande et al., 1999)

sex comb

(Deshpande et al., 1999)

Detailed Description

Statement

Reference

Females carrying Sxl^{N.Hsp83.T:Ecol\lacZ} show only 87.9% viability.

(Penn et al., 2008)

At 25^oC, the viability of females carring Sxl^{N.Hsp83.T:Ecol\lacZ} is reduced by approximately 25% compared to males carrying Sxl^{N.Hsp83.T:Ecol\lacZ}. This female lethal effect is enhanced at 29^oC and suppressed at 18^oC. The viability of males carrying one or two copies of Sxl^{N.Hsp83.T:Ecol\lacZ} is at most only slightly less than that of males that do not carry Sxl^{N.Hsp83.T:Ecol\lacZ}. In contrast, the viability of females carrying one copy of Sxl^{N.Hsp83.T:Ecol\lacZ} is reduced by about 15% compared to females that do not carry Sxl^{N.Hsp83.T:Ecol\lacZ}, while it is reduced by nearly 25% in females carrying two copies of Sxl^{N.Hsp83.T:Ecol\lacZ}. The surviving females are usually phenotypically wild type, although occasionally weak transformations are seen. The viability of females carring Sxl^{N.Hsp83.T:Ecol\lacZ} is further reduced if they are also heterozygous for Sxl^fP7B0. Females carrying two copies of Sxl^{N.Hsp83.T:Ecol\lacZ} and heterozygous for Sxl^fP7B0 have reduced viability compared to females carrying one copy of Sxl^{N.Hsp83.T:Ecol\lacZ} and heterozygous for Sxl^fP7B0. 10% of males carrying Sxl^{N.Hsp83.T:Ecol\lacZ} show phenotypes characteristic of sex transformations, including reduced or patchy abdominal pigmentation, extra sternite hairs, rotated genitalia and alterations in the number or shape of sex combs. The frequency of partially feminised males increases as the temperature decreases.

(Deshpande et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Sxl^N.Hsp83.lacZ, Sxl^fP7B0 has viable phenotype, suppressible | partially by Myc^dm-1

(Kappes et al., 2011)

Sxl^N.Hsp83.lacZ has partially lethal | dominant | female phenotype, suppressible by Df(2R)CX1

(Penn et al., 2008)

Sxl^N.Hsp83.lacZ has partially lethal | dominant | female phenotype, suppressible by fl(2)d[+]/fl(2)d¹

(Penn et al., 2008)

Other

Statement

Reference

Sxl^N.Hsp83.lacZ, snf¹ has lethal | female phenotype

(Deshpande et al., 1999)

Sxl^N.Hsp83.lacZ, snf¹ has partially lethal | dominant | female phenotype

(Deshpande et al., 1999)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Df(1)dm75e19 females crossed with Sxl^{N.Hsp83.T:Ecol\lacZ} males generate Df(1)dm75e19/+ females with 75% of the viability found in their wild-type siblings.

Sxl^{N.Hsp83.T:Ecol\lacZ} females that are trans-heterozygous for Sxl^fP7B0 and dm¹ are two-thirds as viable as their Sxl^{N.Hsp83.T:Ecol\lacZ} Sxl^fP7B0/+ sisters.

(Kappes et al., 2011)

The reduced viability of females carrying Sxl^{N.Hsp83.T:Ecol\lacZ} is completely suppressed by fl(2)d¹/+.

(Penn et al., 2008)

Heterozygous snf¹ females are fully viable. This viability is reduced if the females also carry Sxl^{N.Hsp83.T:Ecol\lacZ}, the viability decreasing as the number of copies of Sxl^{N.Hsp83.T:Ecol\lacZ} increase.

(Deshpande et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Sxl^{N.Hsp83.T:Ecol\lacZ}

Sxl^N.Hsp83.lacZ

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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