FlyBase Allele Report: Dmel\Axn[UAS.cHa]

General Information

Symbol

Dmel\Axn^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hamada

FlyBase ID

FBal0097413

Feature type

allele

Associated gene

Dmel\Axn

Associated Insertion(s)

Carried in Construct

P{UAS-Axn.H}

Also Known As

UAS-Axin, UAS-Daxin

Key Links

Transgenic product class

wild_type

(Hamada et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hamada et al., 1999)

Mutagen

in vitro construct

(Hamada et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Axn.H}

Cytology

Description

The entire Axn open reading frame is expressed under the control of UASt regulatory sequences.

(Hamada et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Axn

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^{neur-GAL4-A101}

(Karim and Moore, 2011)

visible, with Scer\GAL4^71B

(Hamada et al., 1999)

visible, with Scer\GAL4^tsh-Gal4-1

(Hamada et al., 1999)

Phenotype Manifest In

abdominal sternite bristle, with Scer\GAL4^tsh-Gal4-1

(Hamada et al., 1999)

adult abdomen, with Scer\GAL4^tsh-Gal4-1

(Hamada et al., 1999)

denticle | increased number, with Axn^S044230, Scer\GAL4^da.G32

(Tolwinski, 2009)

embryonic/larval dorsal tracheal branch, with Scer\GAL4^btl.PS

(Chihara and Hayashi, 2000)

embryonic/larval tracheal dorsal trunk, with Scer\GAL4^btl.PS

(Chihara and Hayashi, 2000)

embryonic/larval tracheal lateral trunk, with Scer\GAL4^btl.PS

(Chihara and Hayashi, 2000)

leg, with Scer\GAL4^tsh-Gal4-1

(Hamada et al., 1999)

leg disc, with Scer\GAL4^Dll-md23

(Kubota et al., 2003)

multidendritic neuron, with Scer\GAL4^{neur-GAL4-A101}

(Karim and Moore, 2011)

sternite, with Scer\GAL4^tsh-Gal4-1

(Hamada et al., 1999)

tracheal tip cell | embryonic stage | increased number, with Scer\GAL4^btl.PS

(Kato et al., 2004)

ventral denticle belt, with Axn^S044230, Scer\GAL4^da.G32

(Tolwinski, 2009)

wing, with Scer\GAL4^71B

(Hamada et al., 1999)

Detailed Description

Statement

Reference

Expression of Axn^Scer\UAS.cHa under the control of Scer\GAL4^{neur-GAL4-A101} results in the loss of posterior-derived MD neurons (e.g. 99% of vbd). On the other hand, vdaB and v'ada are never lost. No SOP1a or SOP4a loss occurs in these flies.

(Karim and Moore, 2011)

Homozygous Axn^S044230 germ-line clone-derived embryos expressing Axn^Scer\UAS.cHa under the control of Scer\GAL4^da.G32 show supernumerary denticles in the ventral embryonic epidermis.

(Tolwinski, 2009)

Expression of Axn^Scer\UAS.cHa under the control of Scer\GAL4^ato.3.6 does not affect dorsal cluster neuron axon extension across the optic chiasm in adult flies.

(Srahna et al., 2006)

Terminal tracheal cells are duplicated in Axn^Scer\UAS.cHa; Scer\GAL4^btl.PS embryos, but have normal morphology and most remain in the posterior compartment (only around 3% are displaced to the adjacent anterior compartment).

(Kato et al., 2004)

When Axn^Scer\UAS.cHa is driven by Scer\GAL4^Dll-md23 the overall size of leg discs is reduced.

(Kubota et al., 2003)

Tracheal dorsal trunk formation is inhibited in embryos expressing Axn^Scer\UAS.cHa under the control of Scer\GAL4^btl.PS. Tracheal cell fusion at the dorsal branch and lateral trunk is also inhibited.

(Chihara and Hayashi, 2000)

Expression of Axn^Scer\UAS.cHa under the control of Scer\GAL4^71B results in notches in the wing. Expression of Axn^Scer\UAS.cHa under the control of Scer\GAL4^tsh-Gal4-1 results in the formation of a supernumerary leg from the ventral surface of the normal leg. Sternite bristles are missing and the entire ventral surface of the abdomen is composed of pleura.

(Hamada et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Axn^UAS.cHa/Scer\GAL4^da.G32 is a suppressor of ventral denticle belt | germline clone phenotype of Apc2^d40, Apc^Q8

(Tolwinski, 2009)

Axn^UAS.cHa/Scer\GAL4^da.G32 is a suppressor of ventral denticle belt | germline clone phenotype of Apc2^d40, Apc^Q8, Axn^S044230

(Tolwinski, 2009)

Axn^UAS.cHa/Scer\GAL4^da.G32 is a suppressor of ventral denticle belt | germline clone phenotype of sgg^M11

(Tolwinski, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Axn^Scer\UAS.cHa under the control of Scer\GAL4^da.G32 blocks the naked-cuticle phenotype of Apc^Q8, Apc2^d40 double mutant embryos.

The ventral denticle belt phenotype of Axn^S044230, Apc^Q8, Apc2^d40 triple mutants is suppressed by expression of Axn^Scer\UAS.cHa under the control of Scer\GAL4^da.G32.

Expression of Axn^Scer\UAS.cHa under the control of Scer\GAL4^da.G32 suppresses the ventral denticle belt phenotype of sgg^M11 mutant embryos.

(Tolwinski, 2009)

The addition of Axn^Scer\UAS.cHa partially restores areas of naked cuticle to Axn^Scer\UAS.cHa, Scer\GAL4^arm.PS mutants. Also an abundance of small denticles are seen.

(Cliffe et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^unspecified/Axn^UAS.cHa partially rescues Axn^S044230

(Hamada et al., 1999)

Fails to rescue

Axn^UAS.cHa/Scer\GAL4^da.G32 fails to rescue Axn^S044230

(Tolwinski, 2009)

Comments

Homozygous Axn^S044230 germ-line clone-derived embryos expressing Axn^Scer\UAS.cHa under the control of Scer\GAL4^da.G32 show supernumerary denticles in the ventral embryonic epidermis.

(Tolwinski, 2009)

Axn^Scer\UAS.cHa expressed under the control of Scer\GAL4^unspecified partially rescues 5 to 10% of Axn^S044230 animals, allowing them to survive to the late third larval instar or pupal stage.

(Hamada et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Axn^Scer\UAS.cHa

Axn^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hamada

(Hamada et al., 1999)

Secondary FlyBase IDs

References (12)

Report Sections

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