FlyBase Allele Report: Dmel\da[lyh]

General Information

Symbol

Dmel\da^lyh

Species

D. melanogaster

Name

FlyBase ID

FBal0095470

Feature type

allele

Associated gene

Dmel\da

Associated Insertion(s)

springer{}da^lyh

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

spontaneous

Nature of the Allele

Allele class

loss of function allele

(Smith and Cronmiller, 1999)

Mutagen

spontaneous

(Smith and Cronmiller, 2001)

Progenitor genotype

Associated Insertion(s)

springer{}da^lyh

Cytology

Description

Insertion of a 7.5kb springer element 113bp into the da intron, producing a characteristic TATA target site duplication. The springer element is inserted in opposite orientation to the direction of da transcription.

(Smith and Cronmiller, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:10,388,640..10,388,640 [-]

(Smith and Cronmiller, 2001, Smith and Cronmiller, 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death

(Smith et al., 2002)

female sterile

(Smith and Cronmiller, 2001, Smith and Cronmiller, 1999)

partially lethal (with da^f75)

(Baker and Cronmiller, 2001)

viable

(Smith and Cronmiller, 2001)

Phenotype Manifest In

egg chamber

(Smith and Cronmiller, 2001)

egg chamber (with da²)

(Smith and Cronmiller, 2001)

female germline stem cell (with Dp(2;Y)B231)

(Smith et al., 2002)

follicle cell | heat sensitive, with da^hs.PSP

(Smith et al., 2002)

germarium

(Smith et al., 2002)

germarium (with Dp(2;Y)B231)

(Smith and Cronmiller, 2001)

germarium region 2b

(Smith et al., 2002)

germarium region 3

(Smith et al., 2002)

germline cyst (with Dp(2;Y)B231)

(Smith et al., 2002)

stalk follicle cell

(Smith et al., 2002, Smith and Cronmiller, 2001)

stalk follicle cell (with da²)

(Smith et al., 2002, Smith and Cronmiller, 2001)

stalk follicle cell (with Dp(2;Y)B231)

(Smith and Cronmiller, 2001)

stalk follicle cell | ectopic | heat sensitive, with da^hs.PSP

(Smith et al., 2002)

Detailed Description

Statement

Reference

90% of ovarioles have no distinct interfollicle stalks in newly eclosed da^lyh females. In the remaining 10% of ovarioles, the posteriormost (first formed) follicle is separated by a stalk from the next youngest cyst, but in these cases the second and third cysts of the ovariole are always contained in a single continuous epithelium. In older mutant females there are extensive defects in the vitellarium, although the overall organisation of the germarium appears to be undisturbed. There is an increased distance between regions 1 and 2b of the germaria compared to wild type. There is no evidence of constriction by somatic cells in the posterior region of region 3 of the germarium, which normally happens to complete follicle individualisation. In extreme cases, as the defects accumulate with age, regions 2b and 3 of the germarium are no longer distinguishable. Most ovarioles have no distinct interfollicle stalks in newly eclosed da²/da^lyh females. da^lyh homozygotes that are also expressing da^hs.PSP show formation of ectopic stalks at the expense of the follicular epithelium in the ovaries; partially exposed germline cysts with stalk-like somatic cells that stretch along the cyst are seen. da^lyh homozygous females that are also carrying Dp(2;Y)B231 show degradation of the germline cysts. In germaria which are depleted of mature cysts, stil-dividing germline cysts, which are usually confined to region 2a, spread posteriorly and become surrounded by somatic cells. At the anterior end of the germarium, stem cells occasionally slip out of the germline niche and are lost. da^lyh females show a drastic reduction in the number of apoptotic cells in the germarium compared to wild type.

(Smith et al., 2002)

da^lyh shows no maternal effects on sex determination in complementation tests with da alleles. da^lyh shows no visible phenotypes in combination with null da alleles and deletions. Homozygous and da^lyh/da² ovaries show multiple follicular defects, such as missing stalks, multicyst follicles and degeneration of late-stage cysts. The phenotype of homozygous ovaries worsens with age. Dp(2;Y)B231 ; da^lyh/da^lyh females have ovarioles defects, including long interfollicular stalks and shrunken germaria that are occasionally attached directly to mid-to-late stage follicles.

(Smith and Cronmiller, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

da^lyh has female sterile phenotype, suppressible by Su(da[lyh])26H6[+]/Su(da^lyh)26H6^26H6

(Smith and Cronmiller, 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

da^lyh/Dp(2;Y)B231 has germarium phenotype, suppressible by Su(da[lyh])26H6[+]/Su(da^lyh)26H6^26H6

(Smith and Cronmiller, 2001)

da^lyh has ovariole phenotype, suppressible by Su(da^lyh)26H6^26H6/Su(da^lyh)26H6^26H6

(Smith and Cronmiller, 2001)

da^lyh has ovariole phenotype, suppressible by Su(da[lyh])26H6[+]/Su(da^lyh)26H6^26H6

(Smith and Cronmiller, 2001)

da^lyh has phenotype, suppressible by Su(da^lyh)26H6^26H6

(Smith and Cronmiller, 1999)

da^lyh has phenotype, suppressible by Su(da^lyh)22C1^22C1

(Smith and Cronmiller, 1999)

da^lyh has phenotype, suppressible by Su(da^lyh)22C6^22C6

(Smith and Cronmiller, 1999)

da^lyh has phenotype, suppressible by Su(da^lyh)26E2^26E2

(Smith and Cronmiller, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

da^lyh shows no maternal effects on sex determination in genetic interaction tests with Sxl, sisA and sc. da^lyh stl^8-1204 double heterozygotes do not show ovariole defects. 85% of Dp(2;Y)cb50 ; da^lyh stl^8-1204/Df(2L)J27 ovarioles are normal, with the remaining 15% only having mild defects.

(Smith and Cronmiller, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

da²

(Smith and Cronmiller, 2001)

Rescued by

da^lyh is rescued by da^hs.PSP

(Smith and Cronmiller, 2001)

Comments

da^lyh fails to complement da² for follicle cell formation. Two copies of da^hs.PSP completely rescue the da^lyh loss of function phenotype at 25^oC. Su(da^lyh)26H6^26H6 dominantly suppresses the da^lyh phenotype, with only 15% of ovarioles showing mild defects in the rescued flies. da^lyh ovaries in a homozygous Su(da^lyh)26H6^26H6 background are completely normal.

(Smith and Cronmiller, 2001)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

This allele is defective predominantly in the somatic cells of the ovary, where it appears to behave like a null.

(Smith and Cronmiller, 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

da^lyh

(Ozdowski et al., 2009)

fs(2)LYH

(Smith and Cronmiller, 1999)

fs(2)lyh

(Smith and Cronmiller, 2001)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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