FB2024_03 , released June 25, 2024
Allele: Dmel\miltk14514b
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General Information
Symbol
Dmel\miltk14514b
Species
D. melanogaster
Name
FlyBase ID
FBal0095289
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Nature of the Allele
Progenitor genotype
Associated Insertion(s)
Cytology
Description

P{lacW} insertion in the first intron.

Allele components
Component
Use(s)
Inserted element
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

egg chamber & mitochondrion | germ-line clone

germline cyst & mitochondrion | germ-line clone

oocyte & mitochondrion | germ-line clone

Detailed Description
Statement
Reference

miltk14514b mutants occasionally survive to adulthood.

Mitochondria aggregate prematurely near and within the oocyte in prefollicular cysts from miltk14514b germ-line clones. The mitochondria produce a giant Balbiani body that fills the ooplasm of the oocyte at follicle formation. Mitochondrial number remains elevated at stage 5, but normalizes by stage 9. However, these mitochondria concentrate abnormally at microtubule minus ends in the anterior corners of the follicles.

The fusomes of miltk14514b cysts are indistinguishable from wild type throughout oogenesis and organelles that associate with the Balbiani body, such as Golgi elements, concentrate and move into the oocyte normally.

miltk14514b females lay eggs with normal mitochondrial DNA content which can form pole cells and that can be fertilized to give rise to viable, fertile adults.

Homozygotes die as adults. They can survive for at least a week, but scarcely move, right themselves with great difficulty and more typically dies soon after eclosion.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
l(2)K14514
miltk14514b
miltl(2)K14514
Name Synonyms
Secondary FlyBase IDs
    References (4)