P{lacW} insertion in the first intron.
egg chamber & mitochondrion | germ-line clone
germline cyst & mitochondrion | germ-line clone
oocyte & mitochondrion | germ-line clone
miltk14514b mutants occasionally survive to adulthood.
Mitochondria aggregate prematurely near and within the oocyte in prefollicular cysts from miltk14514b germ-line clones. The mitochondria produce a giant Balbiani body that fills the ooplasm of the oocyte at follicle formation. Mitochondrial number remains elevated at stage 5, but normalizes by stage 9. However, these mitochondria concentrate abnormally at microtubule minus ends in the anterior corners of the follicles.
The fusomes of miltk14514b cysts are indistinguishable from wild type throughout oogenesis and organelles that associate with the Balbiani body, such as Golgi elements, concentrate and move into the oocyte normally.
miltk14514b females lay eggs with normal mitochondrial DNA content which can form pole cells and that can be fertilized to give rise to viable, fertile adults.
Homozygotes die as adults. They can survive for at least a week, but scarcely move, right themselves with great difficulty and more typically dies soon after eclosion.
Complements: snRNP70K02107. Fails to complement: l(2)k06502k06502.