P{lacW} insertion in the first intron.
egg chamber & mitochondrion | germ-line clone
follicle cell & mitochondrion | germ-line clone
germline cyst & mitochondrion | germ-line clone
oocyte & mitochondrion | germ-line clone
Mitochondria aggregate prematurely near and within the oocyte in prefollicular cysts from miltk06704 germ-line clones. The mitochondria produce a giant Balbiani body that fills the ooplasm of the oocyte at follicle formation. Mitochondrial number remains elevated at stage 5, but normalizes by stage 9. However, these mitochondria concentrate abnormally at microtubule minus ends in the anterior corners of the follicles.
The fusomes of miltk06704 cysts are indistinguishable from wild type throughout oogenesis and organelles that associate with the Balbiani body, such as Golgi elements, concentrate and move into the oocyte normally.
miltk06704 females lay eggs with normal mitochondrial DNA content which can form pole cells and that can be fertilized to give rise to viable, fertile adults.
Mitochondria cluster at the apical side of miltk06704 young mutant follicle cells (from germline clones) but the mitochondrial distribution is normal in follicles older than stage 7. In miltk06704 mutant germline stem cells, all the mitochondria are clumped at the opposite side from the spectrosome, instead of being clustered around the spectrosome as in wild-type stem cells.
Homozygotes die as adults. They can survive for at least a week, but scarcely move, right themselves with great difficulty and more typically dies soon after eclosion.
I. Kiss.
Complements: snRNP70K02107.