FB2024_03 , released June 25, 2024
Allele: Dmel\Vps36L5212
Open Close
General Information
Symbol
Dmel\Vps36L5212
Species
D. melanogaster
Name
FlyBase ID
FBal0087016
Feature type
allele
Associated gene
Dmel\Vps36
Associated Insertion(s)
P{lacW}Vps36L5212
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(BDGP Project Members, 1994-1999)
Progenitor genotype
Associated Insertion(s)
P{lacW}Vps36L5212
Cytology
Description

Insertion 29bp upstream of the ATG codon of Vps36.

(Irion and St Johnston, 2007)
Allele components
Component
Use(s)
Inserted element
P{lacW}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA
      (Herz et al., 2009)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Vps36L5212 somatic clones in eye-antennal imaginal discs contain enlarged, abnormal early endosomes.

      Vps36L5212 somatic clones in eye-antennal imaginal discs show high levels of apoptosis compared to wildtype. Eye-antennal imaginal discs are comparable in size to wildtype, however in the adult a rough-eye phenotype is seen.

      When the majority of cells in the eye-antennal imaginal discs are mutant for Vps36L5212, striking tissue overgrowth of the disc occurs and loose cellular architecture is observed.

      Cell proliferation does occur in Vps36L5212 somatic clones in eye-antennal imaginal discs, and clones are unable to induce non-autonomous proliferation in surrounding wildtype tissue.

      (Herz et al., 2009)

      Females with homozygous germline clones do not lay eggs.

      (Irion and St Johnston, 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Statement
      Reference

      Vps36L5212 has eye | somatic clone phenotype, non-suppressible by N[+]/N264-39

      (Herz et al., 2009)
      Suppressor of
      Statement
      Reference

      Vps36L5212 is a suppressor of eye phenotype of hidGMR.PG

      (Herz et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Heterozygosity for N264-39 does not suppresses the rough-eye phenotype that occurs in animals with somatic clones of Vps36L5212 generated in the eye-antennal disc.

      The eye-ablation phenotype in WGMR.PG animals is strongly suppressed in Vps36L5212 somatic clones generated in the eye-antennal imaginal disc.

      (Herz et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Vps36L5212
      l(3)L5212
      (Irion and St Johnston, 2007, Spradling et al., 1999)
      l(3)L5212L5212
      vps36L5212
      (Herz et al., 2009, Irion and St Johnston, 2007)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)