FlyBase Allele Report: Dmel\ush[VX22]

General Information

Symbol

Dmel\ush^VX22

Species

D. melanogaster

Name

FlyBase ID

FBal0082099

Feature type

allele

Associated gene

Dmel\ush

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Biryukova and Heitzler, 2008, Cubadda et al., 1997)

Mutagen

X ray

(Biryukova and Heitzler, 2008)

Progenitor genotype

Cytology

Description

Frameshift after P165 resulting in a truncated (171 aa) protein, lacking the nuclear localisation site and zinc finger domains.

(Biryukova and Heitzler, 2008)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

sequence_alteration

2L:536,451..536,451 [+]

Comment:

Location of unspecified frameshift event (after P165 of ush-PA), which results in a truncated protein.

(Biryukova and Heitzler, 2008)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult thorax | dorsal | somatic clone

(Cubadda et al., 1997)

dorsocentral bristle | cell non-autonomous | somatic clone

(Cubadda et al., 1997)

embryonic/larval crystal cell | larval stage

(Sorrentino et al., 2007)

embryonic/larval crystal cell | third instar larval stage

(Gao et al., 2009)

embryonic/larval lymph gland (with ush^rev24)

(Tokusumi et al., 2010)

embryonic/larval lymph gland | larval stage

(Sorrentino et al., 2007)

embryonic/larval lymph gland | second instar larval stage (with ush^rev24)

(Sorrentino et al., 2007)

embryonic/larval lymph gland | third instar larval stage

(Gao et al., 2009)

embryonic/larval lymph gland | third instar larval stage (with ush^rev24)

(Gao et al., 2009)

embryonic/larval plasmatocyte | third instar larval stage

(Gao et al., 2009)

embryonic/larval plasmatocyte | third instar larval stage (with ush^rev24)

(Gao et al., 2009)

hemocyte | larval stage

(Sorrentino et al., 2007)

hemocyte | third instar larval stage (with ush^rev24)

(Sorrentino et al., 2007)

lamellocyte (with ush^rev24)

(Tokusumi et al., 2010)

lamellocyte | larval stage

(Sorrentino et al., 2007)

lamellocyte | third instar larval stage

(Gao et al., 2009)

lamellocyte | third instar larval stage (with ush^rev24)

(Gao et al., 2009, Sorrentino et al., 2007)

scutellum | somatic clone

(Cubadda et al., 1997)

Detailed Description

Statement

Reference

ush^VX22/ush^rev24 mutant larvae exhibit an over-production of lamellocytes in the lymph glands.

(Tokusumi et al., 2010)

ush^VX22/+ third instar larval lymph glands exhibit hyperplasia, and the lymph glands are on average twice the size of wildtype glands. Plasmatocyte and crystal cell numbers are substantially increased in ush^VX22/+ lymph glands. Only a marginal increase in lamellocyte differentiation is observed.

ush^VX22/ush^rev24 third instar larval lymph glands exhibit hyperplasia. Lymph glands are on average two times larger in size compared to ush^VX22/+ larvae, and three times larger in size compared to wildtype controls. Plasmatocyte and crystal cell numbers are decreased in ush^VX22/ush^rev24 lymph glands. A substantial increase in lamellocyte differentiation is observed.

(Gao et al., 2009)

ush^VX22 homozygous embryos are U-shaped.

ush^VX22/+ flies have the wildtype number of dorsocentral bristles.

(Biryukova and Heitzler, 2008)

Lymph glands from ush^VX22/ush^rev24 second instar larvae have a comparable size to control glands. In third instar larvae, lymph glands are extremely hypertrophic compared to controls. There is a four-fold increase in circulating haemocytes, including a large lamellocyte population which comprises about 20% of all circulating haemocytes.

The lymph glands of ush^VX22/+ larvae are hypertrophic. Crystal cells are still predominantly confined to the cortical zone, but are less densely packed. Larvae have a small but significant circulating lamellocyte population, compared to a almost no lamellocytes circulating in wild-type larvae. The number of non-lamellocyte cells is much increased compared to control larvae.

(Sorrentino et al., 2007)

Homozygous clones in the lateral part of the notum differentiate normally. Clones that extend into the scutellum fail to differentiate, producing large gaps in this region. Clones touching the dorsal midline are associated with a cleft in the thorax, and clones extending into the dorsocentral area are associated with a lack or mispositioning of the dorsocentral bristles. Dorsocentral bristles produced by wild-type cells in flies with clones in part of the dorsocentral area are also misplaced from their normal positions.

(Cubadda et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

ush^VX22 has lamellocyte | larval stage phenotype, suppressible by srp³/srp[+]

(Sorrentino et al., 2007)

ush^VX22 has lamellocyte | larval stage phenotype, suppressible by srp^neo45/srp[+]

(Sorrentino et al., 2007)

ush^VX22 has hemocyte | larval stage phenotype, suppressible by srp^neo45/srp[+]

(Sorrentino et al., 2007)

NOT suppressed by

Statement

Reference

ush^VX22 has hemocyte | larval stage phenotype, non-suppressible by srp³/srp[+]

(Sorrentino et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

ush^VX22/+; CtBP^rev19/+ flies have the wildtype number of dorsocentral bristles.

(Biryukova and Heitzler, 2008)

Lamellocyte production is eliminated in ush^VX22/+, srp³/+ double heterozygous mutant larvae, reducing numbers to wild-type levels. The number of non-lamellocyte cells is not affected.

Lamellocyte production is drastically reduced in ush^VX22/+, srp^neo45/+ double heterozygous mutant larvae, but not quite to wild-type levels. The number of non-lamellocyte cells is reduced to almost half the wild-type value.

(Sorrentino et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

ush^VX22 is partially rescued by ush^tCa

(Cubadda et al., 1997)

Comments

Lethality is rescued by ush^tCa, although the rescued flies have a bristle phenotype similar to ush^rev24.

(Cubadda et al., 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ush^VX22

(Moussalem et al., 2021, Fromental-Ramain et al., 2010, Sorrentino, 2010, Tokusumi et al., 2010, Biryukova and Heitzler, 2008, Letizia et al., 2007, Sorrentino et al., 2007)

ush^vx22

(Lenz et al., 2021, Baldeosingh et al., 2018, Gao et al., 2009, Hyun et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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