FlyBase Allele Report: Dmel\srp[3]

General Information

Symbol

Dmel\srp³

Species

D. melanogaster

Name

FlyBase ID

FBal0016082

Feature type

allele

Associated gene

Dmel\srp

Associated Insertion(s)

Carried in Construct

Also Known As

srp^9L, srp^9L06

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Sam et al., 1996)

hypomorphic allele - genetic evidence

(Rehorn et al., 1996)

Mutagen

ethyl methanesulfonate

(Rehorn et al., 1996, Sam et al., 1996, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

N29 of the consensus sequence is replaced by a Lys within the second Cys pair of the zinc finger motif.

(Rehorn et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:16,001,847..16,001,847 [+]

Nucleotide change:

T16001847R

Amino acid change:

Reported amino acid change:

N511K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Rehorn et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | embryonic stage

(Rehorn et al., 1996)

decreased cell number | embryonic stage (with srp^neo45)

(Rehorn et al., 1996)

decreased occurrence of cell division | embryonic stage

(Rehorn et al., 1996)

lethal | recessive

(Rehorn et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

srp³ mutant stage 13 embryos exhibit decreased efferocytosis, as shown by the decreased number of apoptotic particles per macrophage,

(Zheng et al., 2021)

Mutant embryos show defects in visceral mesoderm elongation and have a strong strong germ band retraction phenotype.

(Wolfstetter et al., 2009)

srp^neo45/srp³ embryos develop the majority of srp-dependent tissues normally (including the fat-body), but lack haemocytes entirely.

(Stramer et al., 2008)

srp³/srp^neo45 embryos lack all hemocytes yet show an entirely normal time course of wound reepithelialization.

(Stramer et al., 2005)

In srp³ embryos, the salivary glands invaginate and migrate posteriorly as in wild type, but are mispositioned. Instead of being fully elongated and parallel to the body wall, srp³ glands are often curled back upon themselves. Lumenal branches are occasionally observed in these mutants.

(Vining et al., 2005)

Crystal cell marker expression is lost in srp³ homozygous embryos.

(Waltzer et al., 2003)

The development of the tracheal dorsal trunk appears to be normal.

(Franch-Marro and Casanova, 2000)

Homozygous embryos show transformation of the anterior midgut to foregut and transformation of the posterior midgut to hindgut. Germ cells are internalised into the forming gut at the beginning of gastrulation. Many germ cells remain trapped in the pocket formed by the abnormal gut, while approximately 50% manage to exit the gut and are found in random positions within the embryo.

(Heller and Steinmann-Zwicky, 1998)

The fat body fails to form and the dorsolateral fat body is partly transformed into gonadal mesoderm.

(Riechmann et al., 1998)

Homozygous and srp³/srp^neo45 transheterozygous embryos are devoid of any mature haemocytes. Fat body precursors are present in homozygous embryos but the cells do not proliferate and do not arrange to form the continuous sheet of cells, early events of fat body differentiation do not take place.

(Rehorn et al., 1996)

Germband retraction and dorsal closure fail to occur in homozygous embryos.

(Strecker et al., 1995)

Mutants become distinguishable from wild type during stage 10 of embryogenesis. Cells of the prospective posterior midgut fail to lose their epithelial character and form a mesenchyme. Instead cells form a large cavity that is contiguous with the hindgut, composed of a columnar epithelium resembling the epithelium of the hindgut. No prospective anterior midgut cells, normally derived from the tip of the ventral furrow, attach to the posterior side of the stomodeum, and the anterior part of the midgut is not formed. The anterior part of the digestive tract becomes a blind-ended tube of ectodermal foregut. The endoderm fails to differentiate. The prospective anterior midgut acquires properties of the ectodermal foregut. Rudimentary Malpighian tubules develop at the correct position. In some older embryos uric acid is detectable within the lumen of the hindgut and epithelial cavity.

(Reuter, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

srp³ has embryonic/larval crystal cell phenotype, non-suppressible by lz^UAS.cBa/Scer\GAL4^twi.PG

(Waltzer et al., 2003)

Suppressor of

Statement

Reference

srp³/srp[+] is a suppressor of lamellocyte | larval stage phenotype of ush^VX22

(Sorrentino et al., 2007)

NOT Suppressor of

Statement

Reference

srp³/srp[+] is a non-suppressor of hemocyte | larval stage phenotype of ush^VX22

(Sorrentino et al., 2007)

Other

Statement

Reference

Bfc^ko, srp³ has embryonic/larval plasmatocyte | embryonic stage 13 phenotype

(Zheng et al., 2021)

hkb², srp³ has embryonic Malpighian tubule phenotype

(Ainsworth et al., 2000)

hkb², srp³ has embryonic/larval midgut primordium phenotype

(Ainsworth et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Bfc^ko, srp³ double heterozygous stage 13 embryos exhibit decreased efferocytosis, as shown by the decreased number of apoptotic particles per macrophage,

(Zheng et al., 2021)

Lamellocyte production is eliminated in ush^VX22/+, srp³/+ double heterozygous mutant larvae, reducing numbers to wild-type levels. The number of non-lamellocyte cells is not affected.

(Sorrentino et al., 2007)

The loss of crystal cell marker expression in srp³ homozygous embryos is not suppressed by lz^Scer\UAS.cBa; Scer\GAL4^twi.PG.

(Waltzer et al., 2003)

hkb² srp³ embryos lack the midgut and Malpighian tubules fail to be specified in the hind gut.

(Ainsworth et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

2485

ru¹ hry¹ Diap1^th-1 st¹ cu¹ srp³ sr¹ e^s ca¹/TM3, Sb¹ Ser¹

Kyoto

107360

ru¹ hry¹ Diap1^th-1 st¹ cu¹ srp³ sr¹ e^s ca¹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

serpent^9L

(Ainsworth et al., 2000)

spt^9L

(Strecker et al., 1995)

srp³

(Moussalem et al., 2021, Zheng et al., 2021, Töpfer et al., 2019, Hernández de Madrid and Casanova, 2018, Shlyakhover et al., 2018, Cook et al., 2008.12.28, Stramer et al., 2008, Gajewski et al., 2007, Sorrentino et al., 2007, Muratoglu et al., 2006, Stramer et al., 2005)

srp^9L06

(Franch-Marro and Casanova, 2000, Goldman-Levi et al., 1996, Sam et al., 1996)

srp^9L

(Wolfstetter et al., 2009, Broihier et al., 1998, Matsubayashi and Yamamoto, 1998, Moore et al., 1998, Moore et al., 1998, Riechmann et al., 1998, Yip et al., 1997, Rehorn et al., 1996, Reuter, 1994, Tearle and Nusslein-Volhard, 1987)

Name Synonyms

Secondary FlyBase IDs

References (32)

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