FlyBase Allele Report: Dmel\fzr[UAS.cSa]

General Information

Symbol

Dmel\fzr^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Sigrist

FlyBase ID

FBal0064876

Feature type

allele

Associated gene

Dmel\fzr

Associated Insertion(s)

Carried in Construct

P{UAS-fzr.S}

Also Known As

UAS-fzr

Key Links

Transgenic product class

cDNA

(Sigrist and Lehner, 1997)

wild_type

(Sigrist and Lehner, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sigrist and Lehner, 1997)

wild_type

(Sigrist and Lehner, 1997)

Mutagen

in vitro construct

(Sigrist and Lehner, 1997)

Progenitor genotype

Carried in construct

P{UAS-fzr.S}

Cytology

Description

fzr cDNA is expressed under the control of UASt regulatory sequences.

(Sigrist and Lehner, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

fzr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^prd.RG1

(Grosskortenhaus and Sprenger, 2002)

abnormal mitotic cell cycle | embryonic stage, with Scer\GAL4^prd.RG1

(Reber et al., 2006)

decreased cell number | adult stage | conditional, with Scer\GAL4^byn-Gal4

(Cohen et al., 2021)

decreased occurrence of cell division | larval stage, with Scer\GAL4^en-e16E

(Sigrist and Lehner, 1997)

increased cell number | adult stage | conditional, with Scer\GAL4^byn-Gal4

(Cohen et al., 2021)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

lethal - all die before end of pupal stage, with Scer\GAL4^en-e16E

(Swan and Schupbach, 2007)

lethal - all die before end of pupal stage, with Scer\GAL4^ptc-559.1

(Swan and Schupbach, 2007)

some die during pupal stage, with Scer\GAL4^en-e16E

(Swan and Schupbach, 2007)

some die during pupal stage, with Scer\GAL4^ptc-559.1

(Swan and Schupbach, 2007)

Phenotype Manifest In

adult head, with Scer\GAL4^ey.PH

(Pimentel and Venkatesh, 2005)

adult midgut, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

adult pyloric epithelium | conditional, with Scer\GAL4^byn-Gal4

(Cohen et al., 2021)

antenna | ectopic, with Scer\GAL4^ey.PH

(Pimentel and Venkatesh, 2005)

antennal primordium, with Scer\GAL4^ey.PH

(Pimentel and Venkatesh, 2005)

cardioblast, with Scer\GAL4^twi.PG

(Han and Bodmer, 2003)

embryonic epidermis, with Scer\GAL4^prd.RG1

(Reber et al., 2006)

embryonic pericardial cell, with Scer\GAL4^twi.PG

(Han and Bodmer, 2003)

eye, with Scer\GAL4^ey.PH

(Pimentel and Venkatesh, 2005)

eye disc, with Scer\GAL4^c527

(Silies et al., 2007)

eye disc, with Scer\GAL4^repo.PU

(Silies et al., 2007)

follicle cell & nucleus | somatic clone, with Scer\GAL4^Act5C.PP

(Shcherbata et al., 2004)

glial cell, with Scer\GAL4^c527

(Silies et al., 2007)

glial cell, with Scer\GAL4^repo.PU

(Silies et al., 2007)

nucleus | adult stage | conditional, with Scer\GAL4^byn-Gal4

(Cohen et al., 2021)

wing disc | larval stage, with Scer\GAL4^en-e16E

(Sigrist and Lehner, 1997)

Detailed Description

Statement

Reference

In individuals with injured gut at late L3W (by the expression of rpr^UAS.cCb), the expression of fzr^UAS.cSa under the control of Scer\GAL4^byn-Gal4 leads to decreased adult pyloric cell number; cell show increased nuclear size.

In individuals with injured gut as adults (by the expression of rpr^UAS.cCb), the expression of fzr^UAS.cSa under the control of Scer\GAL4^byn-Gal4 leads to increased pyloric cell number.

(Cohen et al., 2021)

Expression of rap^Scer\UAS.cSa under the control of Scer\GAL4^repo.PU or Scer\GAL4^c527 results in a dramatic reduction in glial cell number in the eye disc.

Expression of rap^Scer\UAS.cSa under the control of Scer\GAL4^Mz97 has no effect on glial cells in the eye disc.

(Silies et al., 2007)

Expression of rap^Scer\UAS.cSa under the control of Scer\GAL4^prd.RG1 causes inhibition of mitosis 16 in the rap^Scer\UAS.cSa-expressing embryonic segments.

(Reber et al., 2006)

Expression of rap^Scer\UAS.cSa in the developing eye, under the control of Scer\GAL4^ey.PH, causes three distinct phenotypes: 1. Inhibition of compound eye development. The severity of this ranges from a drastic reduction of the eye to a complete loss of the eye. 2. Induction of ectopic antennae. These antennae can appear fully-differentiated, or may consist of only the first antennal segment. They can occur at the eye position or surrounding regions of the head. Staining of the eye-antennal disc with antennal-specific markers suggests that this ectopic formation is due to an increase in size of the antennal primordia. 3. Induction of abnormally large cells in the eye and more medial regions of the head that form tumour-like structures.

(Pimentel and Venkatesh, 2005)

In somatic clones of rap^Scer\UAS.cSa; Scer\GAL4^Act5C.PP cells in the ovarian follicle cells, mitosis arrests (indicated by loss of expression of some mitotic markers) and nuclei become enlarged. FACS profiles of DNA content in follicle cell populations containing these somatic clones show a 32n population not present in wild-type. However, amplification at specific loci (seen after stage 10B in wild-type) occurs normally in these enlarge nuclei.

(Shcherbata et al., 2004)

When rap^Scer\UAS.cSa is driven by Scer\GAL4^twi.PG, most hemisegments of mutant embryos show only one eve expressing pericardial cell (EPC), suggesting that the last division generating two EPCs did not occur. Only four (instead of the normal six) myocardial cells form per hemisegment.

(Han and Bodmer, 2003)

In rap^Scer\UAS.cSa; Scer\GAL4^prd.RG1 embryos, cells in alternate segments fail to execute mitotic cycle 16, resulting in fewer cells per segment in embryos older than stage 12.

(Grosskortenhaus and Sprenger, 2002)

Mitosis 16 is blocked in embryos expressing rap^Scer\UAS.cSa under the control of Scer\GAL4^prd.RG1. Inhibits mitotic divisions during imaginal disc cell proliferation when expressed in the posterior compartment of the imaginal discs using Scer\GAL4^en-e16E, with the cells undergoing DNA overreplication.

(Sigrist and Lehner, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^prd.RG1, fzr^UAS.cSa has abnormal mitotic cell cycle | embryonic stage phenotype, suppressible by CycA^UAS.cWa, Scer\GAL4^prd.RG1

(Reber et al., 2006)

Scer\GAL4^prd.RG1, fzr^UAS.cSa has abnormal mitotic cell cycle phenotype, suppressible by Rca1^UAS.Tag:HA, Scer\GAL4^prd.RG1

(Grosskortenhaus and Sprenger, 2002)

Suppressor of

Statement

Reference

Scer\GAL4^fkh.PH/fzr^UAS.cSa is a suppressor of increased occurrence of cell division | embryonic stage phenotype of hkb³⁵¹

(Matthew et al., 2024)

Scer\GAL4^fkh.PH/fzr^UAS.cSa is a suppressor | partially of increased cell death | embryonic stage phenotype of hkb³⁵¹

(Matthew et al., 2024)

Scer\GAL4^fkh.PH/fzr^UAS.cSa is a suppressor | partially of decreased size | embryonic stage phenotype of hkb³⁵¹

(Matthew et al., 2024)

Scer\GAL4^fkh.PH, fzr^UAS.cSa, BacA\p35^UAS.cHa is a suppressor of decreased size | embryonic stage phenotype of hkb³⁵¹

(Matthew et al., 2024)

fzr^UAS.cSa, Scer\GAL4^NP7397 is a suppressor of increased occurrence of cell division phenotype of N^GD14477, Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^fkh.PH, fzr^UAS.cSa, hkb³⁵¹ has visible | embryonic stage phenotype

(Matthew et al., 2024)

Scer\GAL4^fkh.PH, fzr^UAS.cSa, hkb³⁵¹ has visible | embryonic stage phenotype

(Matthew et al., 2024)

CycE^AR95, Scer\GAL4^prd.RG1, fzr^UAS.cSa has decreased occurrence of cell division | embryonic cycle 16 phenotype

(Sigrist and Lehner, 1997)

CycE^UAS.cLa, Scer\GAL4^prd.RG1, fzr^UAS.cSa has increased occurrence of cell division | embryonic cycle 16 phenotype

(Sigrist and Lehner, 1997)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^prd.RG1, fzr^UAS.cSa has embryonic epidermis phenotype, suppressible by CycA^UAS.cWa, Scer\GAL4^prd.RG1

(Reber et al., 2006)

Suppressor of

Statement

Reference

Scer\GAL4^fkh.PH, fzr^UAS.cSa, BacA\p35^UAS.cHa is a suppressor of embryonic/larval salivary gland | embryonic stage phenotype of hkb³⁵¹

(Matthew et al., 2024)

Scer\GAL4^fkh.PH/fzr^UAS.cSa is a suppressor | partially of embryonic/larval salivary gland | embryonic stage phenotype of hkb³⁵¹

(Matthew et al., 2024)

fzr^UAS.cSa, Scer\GAL4^NP7397 is a suppressor of midgut phenotype of N^GD14477, Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^fkh.PH, fzr^UAS.cSa has embryo phenotype

(Matthew et al., 2024)

BacA\p35^UAS.cHa, Scer\GAL4^fkh.PH, fzr^UAS.cSa, hkb³⁵¹ has embryo phenotype

(Matthew et al., 2024)

Scer\GAL4^fkh.PH, fzr^UAS.cSa, hkb³⁵¹ has embryo phenotype

(Matthew et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of CycA^Scer\UAS.cWa with rap^Scer\UAS.cSa, under the control of Scer\GAL4^prd.RG1, suppresses the inhibition of embryonic mitosis 16 seen when rap^Scer\UAS.cSa is expressed alone.

(Reber et al., 2006)

Rca1^{Scer\UAS.T:Ivir\HA1} suppresses the embryonic cycle 16 mitotic block due to a rap^Scer\UAS.cSa; Scer\GAL4^prd.RG1.

(Grosskortenhaus and Sprenger, 2002)

Embryos expressing both CycE^Scer\UAS.cLa and rap^Scer\UAS.cSa under the control of Scer\GAL4^prd.RG1 show normal development until and including mitosis 16 followed by an extra division cycle. Results in an inhibition of mitosis when expressed in CycE^AR95 embryos using Scer\GAL4^prd.RG1.

(Sigrist and Lehner, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^elav-C155/fzr^UAS.cSa partially rescues fzr^ie28

(Silies and Klämbt, 2010)

Scer\GAL4^drl-PGAL8/fzr^UAS.cSa partially rescues fzr^x-3

(Pimentel and Venkatesh, 2005)

Fails to rescue

Scer\GAL4^repo.PU/fzr^UAS.cSa fails to rescue fzr^ie28

(Silies and Klämbt, 2010)

Scer\GAL4^gcm.PU/fzr^UAS.cSa fails to rescue fzr^ie28

(Silies and Klämbt, 2010)

Comments

Expression of rap^Scer\UAS.cSa under the control of either Scer\GAL4^gcm.PU or Scer\GAL4^repo.PU fails to rescue the increase in glial cell number and glial cell migration defects seen in rap^ie28 embryos.

Expression of rap^Scer\UAS.cSa under the control of Scer\GAL4^elav-C155 does not rescue the increase in glial cell number seen in rap^ie28 embryos, but the glial cell migration defect is completely rescued in 49.6% of hemisegments and is partially rescued in 25.9% of hemisegments. The axonal growth defects seen in the central and peripheral nervous systems are also rescued.

Expression of rap^Scer\UAS.cSa under the control of Scer\GAL4^elav.PLu almost completely rescues both the glial cell migration defects (71.5% complete rescue, 19% partial rescue) and increase in glial cell number seen in rap^ie28 embryos. The axonal growth defects seen in the central and peripheral nervous systems are also rescued.

(Silies and Klämbt, 2010)

Expression of rap^Scer\UAS.cSa, under the control of Scer\GAL4^drl-PGAL8, fully rescues the rough eye phenotype in 67% of flies and mediates a partial rescue in the other 33%.

(Pimentel and Venkatesh, 2005)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

91688

w^*; P{UAS-fzr.S}II.1

91689

w^*; P{UAS-fzr.S}III.2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

fzr^Scer\UAS.cSa

fzr^UAS.cSa

rap^Scer\UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sigrist

(Sigrist and Lehner, 1997)

Secondary FlyBase IDs

References (17)

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