FlyBase Allele Report: Dmel\Phm[k07623]

General Information

Symbol

Dmel\Phm^k07623

Species

D. melanogaster

Name

FlyBase ID

FBal0061687

Feature type

allele

Associated gene

Dmel\Phm

Associated Insertion(s)

P{lacW}Phm^k07623

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Jiang et al., 2000)

Mutagen

P-element activity

(Kolhekar et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}Phm^k07623

Cytology

Description

Insertion of a P{lacW} element within the coding region, near the predicted signal sequence.

(Kolhekar et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:23,988,247..23,988,254 [-]

(Taghert et al., 2001, Jiang et al., 2000, Spradling et al., 1999, Kolhekar et al., 1997, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | dominant | third instar larval stage

(Knight et al., 2015)

lethal

(Knight et al., 2015, Ray et al., 2015)

lethal | recessive

(Spradling et al., 1999, Kolhekar et al., 1997)

some die during embryonic stage | recessive

(Jiang et al., 2000)

some die during larval stage | recessive

(Jiang et al., 2000)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage

(Knight et al., 2015)

mouthpart | increased number

(Jiang et al., 2000)

Detailed Description

Statement

Reference

Phm^k07623/+ larvae show a significant increase in stimulus evoked transmitter release (no difference in spontaneous mEJPs) and a significant decrease in paired pulse plasticity (20ms or 100ms inter-stimulus interval) at the neuromuscular junction, compared to controls.

(Knight et al., 2015)

Mutants do not show defects in larval locomotory behaviour.

(Suster et al., 2003)

Approximately 50% of homozygote animals fail to hatch. Heterozygous larvae appear normal in their general coordination, locomotory steps and feeding movements. Their response to tactile stimulation and molting behaviour is also normal. Homozygotes also appear normal immediately after emergence. However over the course of the next 24 hours both homozygotes and hemizygotes become lethargic and typically die at or near the end of the first larval instar. When reared at room temperature the longest lived mutant reach 7 days of age, just after molting to the third instar larva. About 75% of dead larvae examined have a double mouthparts phenotype. More than half of Phm^k07623 mutants appear to die while attempting to molt to the second larval instar.

(Jiang et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions