FB2024_03 , released June 25, 2024
Allele: Dmel\PhmUAS.cTA
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General Information
Symbol
Dmel\PhmUAS.cTA
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Taghert
FlyBase ID
FBal0126508
Feature type
allele
Associated gene
Dmel\Phm
Associated Insertion(s)
Carried in Construct
P{UAS-Phm.T}
Key Links
Transgenic product class
cDNA
(Taghert et al., 2001)
wild_type
(Taghert et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Taghert et al., 2001)
wild_type
(Taghert et al., 2001)
Mutagen
in vitro construct
(Taghert et al., 2001)
Progenitor genotype
Carried in construct
P{UAS-Phm.T}
Cytology
Description

An EcoRI/KpnI fragment of the Phm cDNA is driven by UASt sequences.

(Taghert et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Phm
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      When PhmScer\UAS.cTA is driven by Scer\GAL4tim.PE or Scer\GAL4dimm-929 in a wild-type background, no degradation of behavioural rhythmicity is seen.

      (Taghert et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4dimm-929, Scer\GAL4386Y, PhmUAS.cTA rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)

      Scer\GAL4dimm-929, Scer\GAL4Appl.G1a, PhmUAS.cTA rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)

      Scer\GAL4elav-C155, Scer\GAL4dimm-929, PhmUAS.cTA rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)

      Scer\GAL4tim.PE, PhmUAS.cTA, Scer\GAL4Kdm2-36Y rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)

      Scer\GAL4tim.PE, Scer\GAL4dimm-929, PhmUAS.cTA rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)
      Partially rescues

      Scer\GAL4dimm-929/PhmUAS.cTA partially rescues PhmP(29)

      (Taghert et al., 2001)

      Scer\GAL4dimm-929/PhmUAS.cTA partially rescues Phmk07623

      (Taghert et al., 2001)

      PhmUAS.cTA/Scer\GAL4Kdm2-36Y partially rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)

      Scer\GAL4386Y/PhmUAS.cTA partially rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)

      Scer\GAL4dimm-929, Scer\GAL4Toll-6-D42, PhmUAS.cTA partially rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)

      Scer\GAL4dimm-929, Scer\GAL4P2.4.Pdf, PhmUAS.cTA partially rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)

      Scer\GAL4dimm-929/PhmUAS.cTA partially rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)

      Scer\GAL4dimm-929, PhmUAS.cTA, Scer\GAL4Kdm2-36Y partially rescues Phmk07623/PhmP(29)

      (Taghert et al., 2001)
      Fails to rescue

      Scer\GAL4tim.PE/PhmUAS.cTA fails to rescue Phmk07623/PhmP(29)

      (Taghert et al., 2001)
      Comments

      Phmk07623/PhmP(29) flies, rescued by PhmScer\UAS.cTA (driven by either Scer\GAL4CG11033-36Y or Scer\GAL4dimm-929) display two peaks of locomotor activity under cycling conditions. They have a normal peak time, locomotor levels accompanying these maxima are sustained, but do not anticipate the morning lights on transition. Phmk07623/PhmP(29) flies, rescued by PhmScer\UAS.cTA (driven by Scer\GAL4386Y) have wild-type locomotor activity under cycling conditions. Phmk07623/PhmP(29) flies, rescued by PhmScer\UAS.cTA (driven by Scer\GAL4CG11033-36Y) under constant conditions (DD) are very weakly rhythmic over days 1-2 and 3-9. 6% of these individuals are rhythmic. and they show a very low signal to noise ration (SNR) (about 0.3). PhmScer\UAS.cTA is driven by Scer\GAL4dimm-929 rescued flies are also poorly rhythmic during DD days 1-2. From days 3-9 these flies are only 3% rhythmic but have a SNR of about 0.8. When PhmScer\UAS.cTA is driven by Scer\GAL4386Y flies activity approaches that of wild-type. PhmP(29) flies, rescued by PhmScer\UAS.cTA (driven by Scer\GAL4dimm-929) display a low percentage rhythmic animals but display a moderately high SNR. Phmk07623 flies, rescued by PhmScer\UAS.cTA (driven by Scer\GAL4dimm-929) have a lower average SNR, but 77% are rhythmic. The addition of Scer\GAL4Appl.G1a or Scer\GAL4P2.4.Pdf to Scer\GAL4Appl.G1a flies that are rescued by Scer\GAL4dimm-929 driven by Scer\GAL4dimm-929 increases the percentage of rhythmic individuals but does not improve the SNR.

      (Taghert et al., 2001)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      PhmScer\UAS.cTA
      PhmUAS.cTA
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Taghert
      (Taghert et al., 2001)
      Secondary FlyBase IDs
        References (1)