FlyBase Allele Report: Dmel\btl[DN.UAS]

General Information

Symbol

Dmel\btl^DN.UAS

Species

D. melanogaster

Name

dominant negative

FlyBase ID

FBal0060485

Feature type

allele

Associated gene

Dmel\btl

Associated Insertion(s)

Carried in Construct

P{DN-R1}

Also Known As

UAS-btl^DN, UAS-FGFR^DN, UAS-Btl-DN

Key Links

Transgenic product class

dominant_negative_variant

(Reichman-Fried and Shilo, 1995)

inframe_deletion

(Reichman-Fried and Shilo, 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Reichman-Fried and Shilo, 1995)

inframe_deletion

(Reichman-Fried and Shilo, 1995)

Mutagen

in vitro construct

(Reichman-Fried and Shilo, 1995)

Progenitor genotype

Carried in construct

P{DN-R1}

Cytology

Description

UASt regulatory sequences drive expression of a dominant-negative form of btl. The encoded protein contains the signal peptide, extracellular and transmembrane domains, but only 100 amino acids of the cytoplasmic tail.

(Reichman-Fried and Shilo, 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

btl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates carcinoma

modeled by Egfr^UAS.cBa, psq^KK111691

(Fereres et al., 2019)

ameliorates cancer

modeled by Ras85D^Q13.UAS

(Tamamouna et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | larval stage, with Scer\GAL4^btl.PS

(Du et al., 2017)

abnormal neuroanatomy, with Scer\GAL4^ato.3.6

(Srahna et al., 2006)

abnormal neuroanatomy, with Scer\GAL4^hs.2sev

(Reichman-Fried and Shilo, 1995)

Phenotype Manifest In

air sac primordium | absent | larval stage, with Scer\GAL4^btl.PS

(Du et al., 2022)

air sac tracheoblast of dorsal air sac primordium, with Scer\GAL4^btl.PS

(Sato and Kornberg, 2002)

axon & dorsal cluster neuron & adult brain, with Scer\GAL4^ato.3.6

(Srahna et al., 2006)

coiled tracheole, with Scer\GAL4^btl.PS

(Weaver and Krasnow, 2008)

embryonic/larval ganglionic tracheal branch, with Scer\GAL4^A9

(Jarecki et al., 1999)

embryonic/larval ganglionic tracheal branch, with Scer\GAL4^btl.PS

(Iordanou et al., 2014)

embryonic/larval optic lobe | larval stage, with Scer\GAL4^btl.PS

(Du et al., 2017)

embryonic/larval tracheal section, with Scer\GAL4^A9

(Jarecki et al., 1999)

embryonic/larval tracheal section, with Scer\GAL4^bs-23.26

(Jarecki et al., 1999)

embryonic/larval tracheal system, with Scer\GAL4^hs.2sev

(Reichman-Fried and Shilo, 1995)

eye disc | larval stage, with Scer\GAL4^btl.PS

(Du et al., 2017)

imaginal tracheoblast | larval stage, with Scer\GAL4^btl.PS

(Du et al., 2017)

larval lateral G tracheal branch, with Scer\GAL4^bs-23.26

(Jarecki et al., 1999)

leg disc | larval stage, with Scer\GAL4^btl.PS

(Du et al., 2017)

midline glial cell, with Scer\GAL4^hs.2sev

(Reichman-Fried and Shilo, 1995)

pupal abdominal tracheal branch | somatic clone, with Scer\GAL4^Act5C.PI, Scer\GAL4^esg-p127

(Chen and Krasnow, 2014)

pupal tracheal system, with Scer\GAL4^btl.PS

(Weaver and Krasnow, 2008)

tracheal branch primordium, with Scer\GAL4^btl.PS

(Cela and Llimargas, 2006)

Detailed Description

Statement

Reference

The expression of btl^DN.Scer\UAS under the control of Scer\GAL4^btl.PS results in tracheoblast cells not migrating into the leg discs and leads to only a few btl-positive neurons localizing in the eye disc and in the brain lobe, as compared to controls.

(Du et al., 2017)

Expression of btl^DN.Scer\UAS in tracheal progenitors and their descendants under the control of Scer\GAL4^esg-p127 and Scer\GAL4^Act5C.PI blocks migration and diminishes or eliminates the formation of pupal abdominal tracheae.

(Chen and Krasnow, 2014)

Scer\GAL4^btl.PS-mediated expression of btl^DN.Scer\UAS results in shorter tracheal ganglionic branches.

(Iordanou et al., 2014)

Expression of btl^DN.Scer\UAS under the control of Scer\GAL4^btl.PS from the late second larval instar stage onwards reduces or eliminates secondary branches and coiled tracheolar (CT) cells in the pupal tracheal system.

(Weaver and Krasnow, 2008)

Embryos that express btl^DN.Scer\UAS under the control of Scer\GAL4^btl.PS do not show tracheal branch integrity defects but do show mild defects in fusion and terminal branching.

(Cela and Llimargas, 2006)

Expression of btl^DN.Scer\UAS under the control of Scer\GAL4^ato.3.6 does not affect initial extension of dorsal cluster neuron axons at 20%-30% pupal development but results in a large increase (from 11.7 to 18.9) in the number of dorsal cluster neuron axons that cross toward the medulla in adult brains.

(Srahna et al., 2006)

The wing discs of third instar btl^DN.Scer\UAS; Scer\GAL4^btl.PS larvae have decreased numbers and migration of air sac tracheoblasts.

(Sato and Kornberg, 2002)

Expression of btl^DN.Scer\UAS under the control of Scer\GAL4^dpp.blk1 has no effect on furrow formation in the eye disc.

(Kumar and Moses, 2001)

btl^DN.Scer\UAS, when driven by Scer\GAL4^A9, leads to significantly lower numbers of GB tracheal branches than seen in wild-type. btl^DN.Scer\UAS when driven by Scer\GAL4^bs-23.26 leads to significantly lower numbers of LG tracheal branches than seen in wild-type.

(Jarecki et al., 1999)

Scer\GAL4^hs.2sev-mediated expression of btl^DN.Scer\UAS 4 hours AEL exerts an inhibitory effect on tracheal and midline glia (MGP) cell migration. Heat shock at different stages of tracheal migration reveals btl is obligatory primarily for the onset of the tracheal migration process. btl is also required for the formation of tracheoles; no tracheoles form in heat treated flies.

(Reichman-Fried and Shilo, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ato.3.6, btl^DN.UAS has abnormal neuroanatomy phenotype, suppressible by Rac1^UAS.cLa, Scer\GAL4^ato.3.6

(Srahna et al., 2006)

Scer\GAL4^ato.3.6, btl^DN.UAS has abnormal neuroanatomy phenotype, suppressible by dsh¹/dsh[+]

(Srahna et al., 2006)

NOT Enhancer of

Statement

Reference

btl^DN.UAS, Scer\GAL4^ato.3.6 is a non-enhancer of abnormal neuroanatomy phenotype of Rac1^UAS.cLa, Scer\GAL4^ato.3.6

(Srahna et al., 2006)

Suppressor of

Statement

Reference

btl^DN.UAS, Scer\GAL4^ap-md544 is a suppressor of neoplasia | larval stage phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

btl^DN.UAS, Scer\GAL4^ap-md544 is a suppressor of increased cell number | larval stage phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

btl^DN.UAS, Scer\GAL4^ap-md544 is a suppressor of increased mortality during development phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

btl^DN.UAS/Scer\GAL4^ato.3.6 is a suppressor of abnormal neuroanatomy phenotype of dsh¹

(Srahna et al., 2006)

NOT Suppressor of

Statement

Reference

btl^DN.UAS, Scer\GAL4^ato.3.6 is a non-suppressor of abnormal neuroanatomy phenotype of Rac1^UAS.cLa, Scer\GAL4^ato.3.6

(Srahna et al., 2006)

Other

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^ap-md544, btl^DN.UAS, psq^KK111691 has lethal - all die before end of pupal stage phenotype

(Fereres et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ato.3.6, btl^DN.UAS has axon & dorsal cluster neuron & adult brain phenotype, suppressible by Rac1^UAS.cLa, Scer\GAL4^ato.3.6

(Srahna et al., 2006)

Scer\GAL4^ato.3.6, btl^DN.UAS has axon & dorsal cluster neuron & adult brain phenotype, suppressible by dsh¹/dsh[+]

(Srahna et al., 2006)

NOT suppressed by

Statement

Reference

Scer\GAL4^btl.PS, btl^DN.UAS has embryonic/larval ganglionic tracheal branch phenotype, non-suppressible by exp¹³⁵

(Iordanou et al., 2014)

NOT Enhancer of

Statement

Reference

btl^DN.UAS, Scer\GAL4^ato.3.6 is a non-enhancer of axon & dorsal cluster neuron phenotype of Rac1^UAS.cLa, Scer\GAL4^ato.3.6

(Srahna et al., 2006)

Suppressor of

Statement

Reference

btl^DN.UAS, Scer\GAL4^ap-md544 is a suppressor of wing disc phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^KK111691

(Fereres et al., 2019)

btl^DN.UAS/Scer\GAL4^btl.PS is a suppressor of embryonic/larval tracheal lateral trunk phenotype of exp¹³⁵

(Iordanou et al., 2014)

btl^DN.UAS/Scer\GAL4^btl.PS is a suppressor of embryonic/larval ganglionic tracheal branch phenotype of exp¹³⁵

(Iordanou et al., 2014)

btl^DN.UAS/Scer\GAL4^ato.3.6 is a suppressor of axon & dorsal cluster neuron phenotype of dsh¹

(Srahna et al., 2006)

NOT Suppressor of

Statement

Reference

btl^DN.UAS, Scer\GAL4^ato.3.6 is a non-suppressor of axon & dorsal cluster neuron phenotype of Rac1^UAS.cLa, Scer\GAL4^ato.3.6

(Srahna et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

All larvae co-expressing psq^KK111691 and Egfr^UAS.cBa under the control of Scer\GAL4^ap-md544 during larval stages (using Gal80[ts] for the temporal regulation of expression) wing discs tumors, associated with higher numbers of myoblasts. These phenotypes are partially suppressed by the co-expression of btl^DN.UAS.

(Fereres et al., 2019)

Scer\GAL4^btl.PS-mediated expression of btl^DN.Scer\UAS suppresses the tracheal cyst phenotype of exp¹³⁵ embryos.

(Iordanou et al., 2014)

Coexpression of Rac1^Scer\UAS.cLa and btl^DN.Scer\UAS, under the control of Scer\GAL4^ato.3.6, results in the dominance of the Rac1^Scer\UAS.cLa phenotype, with a reduction in the number of dorsal cluster neuron axons crossing toward the medulla.

Coexpression of btl^DN.Scer\UAS and Rac1^N17.Scer\UAS, under the control of Scer\GAL4^ato.3.6, results in all dorsal cluster neuron axons crossing the optic chiasm, instead of the majority retracting and branching within the lobula. This indicates that the Rac1^N17.Scer\UAS phenotype is dominant.

Expression of btl^DN.Scer\UAS under the control of Scer\GAL4^ato.3.6 in a dsh¹/+ background suppresses the reduction of dorsal cluster neuron axon extension phenotype seen in either single mutant, with the number of dorsal cluster neuron axons crossing the optic chiasm restored to almost wild-type levels.

(Srahna et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

btl^DN.Scer\UAS

btl^DN.UAS

btl^DN

(Weaver and Krasnow, 2008)

Name Synonyms

dominant negative

(Reichman-Fried and Shilo, 1995)

Secondary FlyBase IDs

References (17)

Report Sections

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