FlyBase Allele Report: Dmel\exp[135]

General Information

Symbol

Dmel\exp¹³⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0298064

Feature type

allele

Associated gene

Dmel\exp

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Iordanou et al., 2014)

Mutagen

Delta2-3 transposase

(Iordanou et al., 2014)

Progenitor genotype

P{EP}PI31^G7971

(Iordanou et al., 2014)

Caused by aberration

Df(2R)exp¹³⁵

Cytology

Description

Imprecise excision of P{EP}PI31^G7971 removing exons 3-11 of exp and potentially generates a severely truncated protein (39 aa) that lacks the entire MH2 and C-terminal domains.

(Iordanou et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval ganglionic tracheal branch

(Iordanou et al., 2014)

embryonic/larval ganglionic tracheal branch (with Df(2R)BSC879)

(Iordanou et al., 2014)

embryonic/larval ganglionic tracheal branch (with exp¹¹)

(Iordanou et al., 2014)

embryonic/larval tracheal lateral trunk

(Iordanou et al., 2014)

embryonic/larval tracheal lateral trunk (with Df(2R)BSC879)

(Iordanou et al., 2014)

embryonic/larval tracheal lateral trunk (with exp¹¹)

(Iordanou et al., 2014)

Detailed Description

Statement

Reference

Homozygous, hemizygous and trans-heterozygous (with exp¹¹) mutant embryos exhibit bubble-like cysts with an enlarged diameter in unicellular (lateral trunk, ganglionic branch) and intracellular (lateral ganglionic branch) tracheal branches, but not in multicellular branches (dorsal trunk) or intracellular fusion branches. Bubbles are also seen in the dorsal branch and visceral branch, but to a lesser extent. Despite this, the tracheal network exhibits normal branching patterns. Tracheal cell number is also unaffected in exp¹³⁵ mutants.

Tracheal cysts in exp¹³⁵ embryos are modified apical extracellular lumen in unicellular branches, and intracellular vacuole-like compartments in intracellular branches.

Defective accumulation of a subset of luminal components is observed in unicellular and intracellular (but not multicellular) tracheal branches where cysts form in exp¹³⁵ mutants.

(Iordanou et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

exp¹³⁵ has embryonic/larval tracheal lateral trunk phenotype, enhanceable by Scer\GAL4^btl.PS/Egfr^Elp.UAS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, enhanceable by Scer\GAL4^btl.PS/Egfr^Elp.UAS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval tracheal lateral trunk phenotype, enhanceable by Scer\GAL4^btl.PS/Raf^UAS.F179

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, enhanceable by Scer\GAL4^btl.PS/Raf^UAS.F179

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval tracheal lateral trunk phenotype, enhanceable by btl^λ.UAS/Scer\GAL4^btl.PS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, enhanceable by btl^λ.UAS/Scer\GAL4^btl.PS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, enhanceable by Rho1^V14.UAS/Scer\GAL4^btl.PS

(Iordanou et al., 2014)

NOT Enhanced by

Statement

Reference

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, non-enhanceable by Cdc42^N17.UAS/Scer\GAL4^btl.PS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval tracheal lateral trunk phenotype, non-enhanceable by Scer\GAL4^btl.PS/tkv^QD.UAS.cUa

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, non-enhanceable by Scer\GAL4^btl.PS/tkv^QD.UAS.cUa

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval tracheal lateral trunk phenotype, non-enhanceable by put¹³⁵

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, non-enhanceable by put¹³⁵

(Iordanou et al., 2014)

Suppressed by

Statement

Reference

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, suppressible by Scer\GAL4^btl.PS/Egfr^DN.UAS.cBa

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval tracheal lateral trunk phenotype, suppressible by btl^DN.UAS/Scer\GAL4^btl.PS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, suppressible by btl^DN.UAS/Scer\GAL4^btl.PS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, suppressible | partially by Scer\GAL4^btl.PS/Cdc42^V12.UAS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, suppressible | partially by Scer\GAL4^btl.PS/Rac1^N17.UAS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, suppressible | partially by Rho1^N19.UAS/Scer\GAL4^btl.PS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval tracheal lateral trunk phenotype, suppressible by Scer\GAL4^btl.PS/Egfr^DN.UAS.cBa

(Iordanou et al., 2014)

NOT suppressed by

Statement

Reference

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, non-suppressible by Cdc42^N17.UAS/Scer\GAL4^btl.PS

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval tracheal lateral trunk phenotype, non-suppressible by Scer\GAL4^btl.PS/tkv^QD.UAS.cUa

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, non-suppressible by Scer\GAL4^btl.PS/tkv^QD.UAS.cUa

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval tracheal lateral trunk phenotype, non-suppressible by put¹³⁵

(Iordanou et al., 2014)

exp¹³⁵ has embryonic/larval ganglionic tracheal branch phenotype, non-suppressible by put¹³⁵

(Iordanou et al., 2014)

NOT Suppressor of

Statement

Reference

exp¹³⁵ is a non-suppressor of embryonic/larval tracheal section phenotype of Egfr^Elp.UAS, Scer\GAL4^btl.PS

(Iordanou et al., 2014)

exp¹³⁵ is a non-suppressor of embryonic/larval ganglionic tracheal branch phenotype of Egfr^DN.UAS.cBa, Scer\GAL4^btl.PS

(Iordanou et al., 2014)

exp¹³⁵ is a non-suppressor of embryonic/larval ganglionic tracheal branch phenotype of Scer\GAL4^btl.PS, btl^DN.UAS

(Iordanou et al., 2014)

exp¹³⁵ is a non-suppressor of embryonic/larval tracheal section phenotype of Scer\GAL4^btl.PS, btl^λ.UAS

(Iordanou et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Scer\GAL4^btl.PS-mediated expression of Egfr^Elp.Scer\UAS or btl^{Scer\UAS.T:λ\cI-DD} enhances, whereas expression of Egfr^{DN.Scer\UAS.cBa} or btl^DN.Scer\UAS suppresses the tracheal cyst phenotype of exp¹³⁵ embryos.

Scer\GAL4^btl.PS-mediated expression of Rho1^V14.Scer\UAS enhances, whereas expression of Cdc42^V12.Scer\UAS or Rac1^N17.Scer\UAS or Rho1^N19.Scer\UAS partially suppresses the tracheal cyst phenotype of exp¹³⁵ embryos. Scer\GAL4^btl.PS-mediated expression of Cdc42^N17.Scer\UAS has no obvious effect on this phenotype.

Neither a put¹³⁵ background nor Scer\GAL4^btl.PS-mediated expression of tkv^{QD.Scer\UAS.cUa} has any effect on the tracheal cyst phenotype of exp¹³⁵ embryos.

(Iordanou et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

exp¹³⁵ is rescued by Scer\GAL4^btl.PS/exp^B.UAS.Tag:HA

(Iordanou et al., 2014)

Comments

Scer\GAL4^btl.PS-mediated expression of exp^{B.Scer\UAS.T:Ivir\HA1} completely rescues the tracheal phenotype of exp¹³⁵ and exp¹¹ mutants.

(Iordanou et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

exp¹³⁵

(Iordanou et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

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