FlyBase Allele Report: Dmel\br[BRcore.NS.Z3.hs]

General Information

Symbol

Dmel\br^{BRcore.NS.Z3.hs}

Species

D. melanogaster

Name

FlyBase ID

FBal0057742

Feature type

allele

Associated gene

Dmel\br

Associated Insertion(s)

Carried in Construct

P{hs-BRC-Z3}

Key Links

Transgenic product class

cDNA

(Crossgrove et al., 1996)

wild_type

(Crossgrove et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Crossgrove et al., 1996)

wild_type

(Crossgrove et al., 1996)

Mutagen

in vitro construct

(Bayer et al., 1997, Sandstrom et al., 1997, Crossgrove et al., 1996)

Progenitor genotype

Carried in construct

P{hs-BRC-Z3}

Cytology

Description

br cDNA dm797 is expressed from a heat inducible Hsp70 promoter.

(Crossgrove et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color | heat sensitive

(Zhou and Riddiford, 2002)

lethal | first instar larval stage | heat sensitive

(Zhou et al., 2004)

lethal | heat sensitive

(Bayer et al., 1997)

lethal | pharate adult stage P8 | heat sensitive

(Zhou et al., 2004)

wild-type

(Bayer et al., 1997)

Phenotype Manifest In

chaeta | heat sensitive

(Zhou and Riddiford, 2002)

dorsal appendage | ectopic | cold sensitive

(Tzolovsky et al., 1999)

egg chorion | cold sensitive

(Tzolovsky et al., 1999)

micropyle | cold sensitive

(Tzolovsky et al., 1999)

Detailed Description

Statement

Reference

Ubiquitous expression of br^{BRcore.NS.Z3.hs} does not cause malrotation of male genitalia.

(Wilson et al., 2006)

83% of larvae expressing four copies of br^Z3.Scer\UAS induced by heat shock at 5 hours after ecdysis to the second instar fail to initiate the third larval moult but continue to eat and grow, far exceeding the size of a normal second instar larva. These larvae eventually pupariate approximately 93 hours after the heat-shock treatment, forming "L2 prepupae" which are characterised by their uneverted, single club-shaped anterior spiracles and small mouthhooks with a few teeth (characteristic of wild-type second instar larvae). Approximately 97% of the L2 prepupae successfully evert their imaginal discs and head, and most of them develop up to stage P8, showing pale yellow pigmentation in the eyes. In a very few cases, they become pharate adults.

Expression of br^{BRcore.NS.Z3.hs} in second instar larvae induced by heat shock at least 7 hours after ecdysis to the second instar does not prevent the animals forming a pharate third instar, although most of the animals do not ecdyse.

Expression of br^{BRcore.NS.Z3.hs} in early first instar larvae by heat shock at 2-5 hours after hatching results in larvae that ecdyse to the second instar, but with an 18 hour delay at 25[o]C.

Expression of br^{BRcore.NS.Z3.hs} in first instar larvae by heat shock at 4 hours after hatching followed by two heat shocks at approximately 16 hours and 28 hours prevents 96% of the larvae from moulting to the second instar.

(Zhou et al., 2004)

Animals carrying four copies of br^{BRcore.NS.Z3.hs} and heat shocked at 48 hours after puparium formation (APF) develop into morphologically normal pharate adults. Animals carrying four copies of br^{BRcore.NS.Z3.hs} and heat shocked at 56 hours APF have essentially full length bristles that are unpigmented.

(Zhou and Riddiford, 2002)

In response to heatshock, ectopic dorsal appendage material is produced in the dorsal anterior region of the eggshell. 3-5 post heatshock none of the eggs produced are abnormal, though only 8% of eggs laid hatch. From 5-24 hours post heatshock 42% of eggs produced are abnormal, and 18% of eggs laid hatch. 24-48 hours post heatshock, about a 1/4 of eggs produced are abnormal, and 90% of eggs laid hatch. Viability is recovered to about 95% in 2 days after heatshock. About 20% of all laid eggs have aberrant micropyles, due to excess chorion formation.

(Tzolovsky et al., 1999)

A 37^oC heat shock causing expression of br^{BRcore.NS.Z3.hs} during the pupal stage results in lethality. Heat shock induced expression of br^{BRcore.NS.Z3.hs} during larval or prepupal stages has no effect.

(Bayer et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

br^{BRcore.NS.Z3.hs} is a non-suppressor of embryonic/larval salivary gland phenotype of ftz-f1⁰³⁶⁴⁹/ftz-f1^ex7

(Yamada et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Salivary gland histolysis is not rescued in ftz-f1^ex7/ftz-f1⁰³⁶⁴⁹ transheterozygotes when br^{BRcore.NS.Z3.hs} is expressed using heat shock at 10 hours after puparium formation.

(Yamada et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

br^{BRcore.NS.Z3.hs} rescues br^2Bc-1

(Bayer et al., 1997)

br^{BRcore.NS.Z3.hs} rescues br^2Bc-2

(Bayer et al., 1997)

Partially rescues

br^{BRcore.NS.Z3.hs} partially rescues br^2Bc-1

(Wilson et al., 2006)

Fails to rescue

br^{BRcore.NS.Z3.hs} fails to rescue br²⁸

(Bayer et al., 1997)

br^{BRcore.NS.Z3.hs} fails to rescue br^rbp-5

(Bayer et al., 1997)

br^{BRcore.NS.Z3.hs} fails to rescue br⁵

(Bayer et al., 1997)

br^{BRcore.NS.Z3.hs} fails to rescue br^npr-3

(Bayer et al., 1997)

br^{BRcore.NS.Z3.hs} fails to rescue br^npr-7

(Bayer et al., 1997)

br^{BRcore.NS.Z3.hs} fails to rescue br^rbp-1

(Bayer et al., 1997)

br^{BRcore.NS.Z3.hs} fails to rescue br^rbp-1

(Sandstrom et al., 1997)

Comments

Expression of the br^{BRcore.NS.Z3.hs} transgene partially rescues the malrotated genitalia phenotype of br^2Bc-2 males; this is inversely related to temperature, with 40% of flies showing the phenotype at 22-23^oC and only 14% showing the phenotype at 25^oC.

(Wilson et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

37292

w¹¹¹⁸; P{hs-BRC-Z3}797

Notes on Origin

Discoverer

Comments

The br Z3 protein isoform encoded by br^{BRcore.NS.Z3.hs} provides full 2Bc⁺ function.

(Bayer et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

br^{BRcore.NS.Z3.hs}

Name Synonyms

Secondary FlyBase IDs

References (12)

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