FB2024_03 , released June 25, 2024
Allele: Dmel\brBRcore.TNT.Q1.Z1.hs
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General Information
Symbol
Dmel\brBRcore.TNT.Q1.Z1.hs
Species
D. melanogaster
Name
FlyBase ID
FBal0057740
Feature type
allele
Associated gene
Dmel\br
Associated Insertion(s)
Carried in Construct
P{hs-BRC-Z1}
Also Known As
hs-BR-Z1
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Bayer et al., 1997, Sandstrom et al., 1997, Crossgrove et al., 1996)
Progenitor genotype
Carried in construct
P{hs-BRC-Z1}
Cytology
Description
Allele components
Component
Use(s)
Encoded product / tool
br
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Over-expression of four copies of the br Z1 isoform (using a line containing both brBRcore.TNT.Q1.Z1.hs and brBRcore.Q1.Z1.hs) using heat shock at 5 hours after ecdysis to the second instar causes lethality at approximately 42-68 hours after the heat shock with no detectable third instar mouthhooks.

      (Zhou et al., 2004)

      Animals carrying four copies of brBRcore.TNT.Q1.Z1.hs that have been heat shocked between 45 and 52 hours after puparium formation (APF) form pharate adults with a transparent cuticle on many areas of the head, thorax and abdomen. The microchaetae and macrochaetae are present, and are sometimes slightly tanned. Pharate adults carrying two copies of brBRcore.TNT.Q1.Z1.hs that have been heat shocked at 36 hours APF and again at 48 hours APF have a thin transparent pupal-like cuticle over the whole animal. The abdomen lacks bristles. Bristles are present on the head and thorax although they are mostly unpigmented. In some cases, a pupal-like wing pad is formed instead of the normal adult wing. Animals carrying two copies of brBRcore.TNT.Q1.Z1.hs and two copies of brBRcore.Q1.Z1.hs that have been heat shocked between 45 and 52 hours APF form pharate adults with a transparent cuticle on many areas of the head, thorax and abdomen. The microchaetae and macrochaetae are present, and are sometimes slightly tanned. Dorsolateral areas of the abdomen sometimes show the beginning of adult melanisation that extends into the anterior region of the tergite where melanisation is not normally found. Animals carrying two copies of brBRcore.TNT.Q1.Z1.hs and two copies of brBRcore.Q1.Z1.hs that have been heat shocked between 30-35 hours APF show truncation of bristles on the head and thorax. Animals carrying two copies of brBRcore.TNT.Q1.Z1.hs and two copies of brBRcore.Q1.Z1.hs that have been heat shocked between 35-39 hours APF show truncation of bristles on the abdomen. Animals carrying two copies of brBRcore.TNT.Q1.Z1.hs and two copies of brBRcore.Q1.Z1.hs that have been heat shocked at 35 or 39 hours APF lack abdominal trichomes. Animals carrying two copies of brBRcore.TNT.Q1.Z1.hs that have been heat shocked between 8 and 17 hours after ecdysis to the second instar form a new cuticle but do not ecdyse.

      (Zhou and Riddiford, 2002)

      0-3 hours post heatshock about 6% of the eggs produced are abnormal, and 94% of eggs laid hatch. 3-5 post heatshock about 3/4 of the eggs produced are abnormal, and only 2% of eggs laid hatch. From 5-24 hours post heatshock about 4/5 of eggs produced are abnormal, and 6% of eggs laid hatch. 24-48 hours post heatshock, about a 3/5 of eggs produced are abnormal, and 72% of eggs laid hatch. Eggs laid 4-6 hours after the heatshock of brBRcore.TNT.Q1.Z1.hs mothers, produced eggs with extra dorsal appendage material in the dorsal anterior region of the eggshells dorsal appendages did not elongate properly leading to fused thickened and shortened dorsal appendages. Heat-shock at the time of dorsal appendage formation, stage 11, leads to an "appendageless" phenotype, or to small fused appendages. Dorsal appendages with abnormal shapes and /or different lengths are observed on eggs at stage 12 and 13 at the time of heatshock. Viability is recovered to 73% in 3 days after heatshock. 3.5 to 4.5 hours post heatshock, BrdU staining reveals prolonged and synchronized endoreplication until late stage 10B, followed by specific amplification of genes in each nucleus. Extra spots of BrdU staining is observed in the nuclei of ovaries, 3 hours after heatshock - 6 to 12 spots per nucleus in about 4/5 of nuclei. 4 spots are seen in wild-type. Abnormal migration of anterior follicle cells is also seen. A gap is formed at the anterior pole as a result of failed migration of the follicle cells. Variation in egg shape is also seen.

      (Tzolovsky et al., 1999)

      A 37oC heat shock causing expression of brBRcore.TNT.Q1.Z1.hs during the third larval instar or early prepupal stage results in lethality within a few hours of the heat shock. Heat shock induced expression of brBRcore.TNT.Q1.Z1.hs during late prepupal or pupal stages has no effect.

      (Bayer et al., 1997)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Salivary gland histolysis is rescued in most ftz-f1ex7/ftz-f103649 transheterozygotes when brBRcore.TNT.Q1.Z1.hs is expressed using heat shock at 10 hours after puparium formation, although other ftz-f1 mutant phenotypes are not rescued in these animals.

      (Yamada et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      brBRcore.TNT.Q1.Z1.hs rescues brrbp-1

      (Bayer et al., 2003)

      brBRcore.TNT.Q1.Z1.hs rescues brrbp-5

      (Bayer et al., 1997)

      brBRcore.TNT.Q1.Z1.hs rescues brrbp-1

      (Bayer et al., 1997)

      brBRcore.TNT.Q1.Z1.hs rescues brrbp-1

      (Sandstrom et al., 1997)
      Fails to rescue

      brBRcore.TNT.Q1.Z1.hs fails to rescue br28

      (Bayer et al., 1997)

      brBRcore.TNT.Q1.Z1.hs fails to rescue br2Bc-1

      (Bayer et al., 1997)

      brBRcore.TNT.Q1.Z1.hs fails to rescue br2Bc-2

      (Bayer et al., 1997)

      brBRcore.TNT.Q1.Z1.hs fails to rescue br5

      (Bayer et al., 1997)

      brBRcore.TNT.Q1.Z1.hs fails to rescue brnpr-3

      (Bayer et al., 1997)

      brBRcore.TNT.Q1.Z1.hs fails to rescue brnpr-7

      (Bayer et al., 1997)
      Comments

      Significant rescue of brrbp-1 mutant flies is observed with one copy of the brBRcore.TNT.Q1.Z1.hs transgene (64% survive to eclosion). Only marginal improvement in rescue (69% compared to 64%) is observed when two copies of brBRcore.TNT.Q1.Z1.hs are used. The majority of the rescued animals have normal legs.

      (Bayer et al., 2003)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Z1 isoforms provide the rbp+ function in prepupal salivary glands.

      (Crossgrove et al., 1996)

      The br Z1 protein isoform encoded by brBRcore.TNT.Q1.Z1.hs provides full rbp+ function.

      (Bayer et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      DmZ1
      (Bayer et al., 2003)
      brBRcore.TNT.Q1.Z1.hs
      Name Synonyms
      Secondary FlyBase IDs
        References (14)