FlyBase Allele Report: Dmel\br[Z2.hs]

General Information

Symbol

Dmel\br^Z2.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0057737

Feature type

allele

Associated gene

Dmel\br

Associated Insertion(s)

Carried in Construct

P{hs-BRC-Z2}

Key Links

Transgenic product class

cDNA

(Sandstrom et al., 1997)

wild_type

(Sandstrom et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sandstrom et al., 1997)

wild_type

(Sandstrom et al., 1997)

Mutagen

in vitro construct

(Bayer et al., 1997, Sandstrom et al., 1997)

Progenitor genotype

Carried in construct

P{hs-BRC-Z2}

Cytology

Description

br cDNA cD5 is expressed from a heat inducible Hsp70 promoter.

(Sandstrom et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | heat sensitive

(Bayer et al., 1997)

lethal - all die before end of larval stage | heat sensitive

(Zhou et al., 2004)

some die during larval stage | heat sensitive

(Zhou et al., 2004)

Phenotype Manifest In

dorsal appendage | ectopic | cold sensitive

(Tzolovsky et al., 1999)

male genitalia, with br⁵

(Wilson et al., 2006)

Detailed Description

Statement

Reference

Ubiquitous expression of br^Z2.hs does not cause malrotation of male genitalia. Partial rescue of br⁵/Y mutants, achieved by driving expression of the br^Z2.hs transgene by three heat shocks at 37^oC starting in the third instar results in small numbers of pharate adults, 93% of which have malrotated genitalia.

(Wilson et al., 2006)

Over-expression of four copies of br^Z2.hs using heat shock at 5 hours after ecdysis to the second instar causes a high rate of lethality at approximately 26 hours after the heat shock (the time of ecdysis from second to third instar). Double mouthhooks are seen in these dead larvae.

(Zhou et al., 2004)

Animals carrying four copies of br^Z2.hs and heat shocked at 56 hours after puparium formation develop into morphologically normal pharate adults.

(Zhou and Riddiford, 2002)

In response to heatshock, ectopic dorsal appendage material is produced in the dorsal anterior region of the eggshell. 3-5 post heatshock about half of the eggs produced are abnormal, 5% of eggs laid hatching. From 5-24 hours post heatshock about 3/4 of eggs produced are abnormal, and 12% of eggs laid hatch. 24-48 hours post heatshock, about a 1/3 of eggs produced are abnormal, and 93% of eggs laid hatch.

(Tzolovsky et al., 1999)

A 37^oC heat shock causing expression of br^Z2.hs during the pupal stage results in lethality. Heat shock induced expression of br^Z2.hs during larval or prepupal stages has no effect.

(Bayer et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

br^Z2.hs is a suppressor | partially of embryonic/larval salivary gland phenotype of ftz-f1⁰³⁶⁴⁹/ftz-f1^ex7

(Yamada et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Salivary gland histolysis is rescued in about half of ftz-f1^ex7/ftz-f1⁰³⁶⁴⁹ transheterozygotes when br^Z2.hs is expressed using heat shock at 10 hours after puparium formation.

(Yamada et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

br^Z2.hs partially rescues br²⁸

(Bayer et al., 1997)

br^Z2.hs partially rescues br^2Bc-1

(Bayer et al., 1997)

br^Z2.hs partially rescues br^2Bc-2

(Bayer et al., 1997)

br^Z2.hs partially rescues br⁵

(Bayer et al., 1997)

br^Z2.hs partially rescues br^npr-3

(Bayer et al., 1997)

br^Z2.hs partially rescues br^npr-7

(Bayer et al., 1997)

Fails to rescue

br^Z2.hs fails to rescue br^rbp-5

(Bayer et al., 1997)

br^Z2.hs fails to rescue br^rbp-1

(Bayer et al., 1997)

br^Z2.hs fails to rescue br^rbp-1

(Sandstrom et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The br Z2 protein isoform encoded by br^Z2.hs provides br⁺ function and partial 2Bc⁺ function.

(Bayer et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

br^Z2.hs

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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