FB2024_03 , released June 25, 2024
Allele: Dpse\l(2)gl+t12.2
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General Information
Symbol
Dpse\l(2)gl+t12.2
Species
D. pseudoobscura pseudoobscura
Name
FlyBase ID
FBal0053678
Feature type
allele
Associated gene
Dpse\l(2)gl
Associated Insertion(s)
Carried in Construct
P{D.ps6/5}
Also Known As
P[l(2)gl]
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Manfruelli et al., 1996, Torok et al., 1993)
Progenitor genotype
Carried in construct
P{D.ps6/5}
Cytology
Description

Construct: Included within a 12.2kb genomic BamHI-EcoRI fragment.

(Torok et al., 1993)
Allele components
Component
Use(s)
Encoded product / tool
Dpse\l(2)gl
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Four copies of P{D.ps6/5} leads to an arrest in early embryogenesis.

      (Bloor and Kiehart, 2001)

      The timing of salivary gland histolysis (and of cellular vacuolisation which precedes the histolysis) is accelerated compared to wild type in D.melanogaster animals carrying four copies of Dpse\l(2)gl+t12.2. The duration of larval, prepupal and pupal development is normal in these animals.

      Two hours after pupariation, the chromosomes of the salivary glands of D.melanogaster animals carrying four copies of Dpse\l(2)gl+t12.2 show none of the major puffs seen in wild-type prepupae of the same age. However, a puff is consistently seen in the 52A region, which usually occurs in very late prepupae.

      (Farkas and Mechler, 2000)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Dpse\l(2)gl+t12.2 is a suppressor of lethal | heat sensitive phenotype of l(2)glts3/Df(2L)net62

      (Manfruelli et al., 1996)

      Dpse\l(2)gl+t12.2 is a suppressor of lethal | heat sensitive phenotype of l(2)glts2/Df(2L)net62

      (Manfruelli et al., 1996)

      Dpse\l(2)gl+t12.2 is a suppressor of lethal | heat sensitive phenotype of Df(2L)net62/l(2)glts1

      (Manfruelli et al., 1996)

      Dpse\l(2)gl+t12.2 is a suppressor of lethal phenotype of l(2)glts1

      (Manfruelli et al., 1996)

      Dpse\l(2)gl+t12.2 is a suppressor of lethal phenotype of l(2)glts2

      (Manfruelli et al., 1996)

      Dpse\l(2)gl+t12.2 is a suppressor of lethal phenotype of l(2)glts3

      (Manfruelli et al., 1996)
      Other
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Dpse\l(2)gl+t12.2 is an enhancer of embryo | dorsal closure stage phenotype of zip2/zip1

      (Franke et al., 2005)
      Suppressor of
      Statement
      Reference

      Dpse\l(2)gl+t12.2 is a suppressor of phenotype of l(2)gl275

      (Torok et al., 1993)

      Dpse\l(2)gl+t12.2 is a suppressor of phenotype of l(2)gl334

      (Torok et al., 1993)

      Dpse\l(2)gl+t12.2 is a suppressor of phenotype of l(2)gl4

      (Torok et al., 1993)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of two copies of Dpse\l(2)gl+t12.2 raises the penetrance of the zip1/zip2 failed dorsal closure phenotype to 100%. Ubiquitous overexpression of zipFL.Scer\UAS.T:Avic\GFP, under the control of the Scer\GAL4sqh.PW, rescues dorsal closure in more than 84% of zip1/zip2; Dpse\l(2)gl+t12.2/Dpse\l(2)gl+t12.2 embryos. 61% of these rescued flies survive to hatch as larvae, 41% survive to pupal stages and 2.8% eclose as adults. Expression of zipFL.Scer\UAS.T:Avic\GFP in the epidermis and a few amnioserosa cells, driven by Scer\GAL4e22c, rescues the zip1/zip2; Dpse\l(2)gl+t12.2/Dpse\l(2)gl+t12.2 dorsal closure phenotype in 88% of embryos. Rescued flies survive to larval stages in 60% of cases and to pupal stages in 21% of cases, although no flies eclose. Expression of zipFL.Scer\UAS.T:Avic\GFP in the amnioserosa, driven by Scer\GAL4c381, rescues the dorsal closure phenotype of Dpse\l(2)gl+t12.2/Dpse\l(2)gl+t12.2 mutants in 82% of embryos. However, none of the rescued embryos survive to larval stages. Similarly, when zipFL.Scer\UAS.T:Avic\GFP expression is driven in the leading edge by Scer\GAL4LE, dorsal closure is rescued in 87% of embryos, but only 1% of flies survive to larval stages. zipFL.Scer\UAS.T:Avic\GFP expression in epidermal stripes, driven by Scer\GAL4en-e16E, rescues dorsal closure in only half of embryos and does not rescue lethality.

      (Franke et al., 2005)

      Completely rescues the lethality of l(2)glts1, l(2)glts2 and l(2)glts3.

      (Manfruelli et al., 1996)

      Completely rescues the mutant phenotype of l(2)gl4 and l(2)gl334.

      (Torok et al., 1993)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Assayed in D.melanogaster for ability to rescue l(2)gl mutant phenotypes.

      (Torok et al., 1993)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Dpse\l(2)gl+t12.2
      Name Synonyms
      Secondary FlyBase IDs
        References (5)