FlyBase Allele Report: Dmel\kuz[e29-4]

General Information

Symbol

Dmel\kuz^e29-4

Species

D. melanogaster

Name

FlyBase ID

FBal0051471

Feature type

allele

Associated gene

Dmel\kuz

Associated Insertion(s)

Carried in Construct

Also Known As

kuz^E29

Key Links

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Rooke et al., 1996)

loss of function allele

(Kamimura et al., 2004, Pan and Rubin, 1997)

Mutagen

P-element activity

(Ashburner et al., 1999.10.12, Rooke et al., 1996)

Progenitor genotype

Cytology

Description

2.4kb deletion near the 5' end of the gene, including DNA in the promoter region and the first and second exons.

(Pan and Rubin, 1997)

Deletion of 2.4kb near the 5' end of kuz, including DNA in the promoter region and the first and second introns.

(Rooke et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

In kuz^e29-4 homozygotes, the larval central brain exhibits ectopically proliferating neuroblasts (i.e. increased numbers in S-phase), compared to controls.

(Sood et al., 2022)

kuz^e29-4 embryos do not show blood-brain barrier or trachea barrier as, like in controls, dye injected into the body cavity does not diffuses into the ventral nerve cord of into the tracheal tubes; the trachea also presents a normal distribution of the septate junction protein, NrxIV.

(Petri et al., 2019)

kuz^e29-4 homozygous embryos do not show any obvious somatic musculature phenotype, as compared to controls.

(Jacobs, 2018.7.9)

kuz^e29-4/kuz^e29-4 mutant embryos show no change in overall neuroblast numbers, but show extra Ap neurons, and extra EL neurons, as compared with controls. These mutants also display increased proliferation of thoracic and abdominal neuroblast daughter cells at stage 14 and 15 (but not stage 12), but no significant difference in proliferation of thoracic or abdominal neuroblasts at stage 12, 14 or 15, as compared with controls.

kuz^e29-4/+ mutant embryos do not display a significant difference in the number of Ap neurons or EL neurons, as compared to controls.

(Bivik et al., 2016)

Hemizygous embryos have an increased number of "Ap" neurons in the NB5-6T lineage.

(Ulvklo et al., 2012)

Stage 16 kuz^e29-4 embryos exhibit thinning of the longitudinal connectives and thickening of the commissures compared to controls. FasII axons ectopically cross the midline.

(Coleman et al., 2010)

kuz^e29-4 mutant embryos exhibit an approximately twofold excess of cardioblasts.

In kuz^e29-4 mutant embryos cardioblasts fail to adopt the final cell morphology. The characteristic bean-like shape of cardioblasts, that normally form the ostia at stage 16/17, is not recognizable. Also, the four tin-expressing cardioblasts, which usually have a flattened, cube-like morphology with a distinct polarisation, exhibit an abnormal morphology.

(Albrecht et al., 2006)

The neuromuscular innervation pattern appears normal in kuz^e29-4/kuz^k01403 larvae.

(Meyer and Aberle, 2006)

Embryos that are maternally and zygotically homozygous kuz^e29-4 show hypertrophy of nervous system elements resulting in the lack of an organised CNS.

(Lieber et al., 2002)

Mitotic clones reaching the wing margin result in notches on the wing blade. Clones in the notum result in clusters of supernumerary macrochaetes and microchaetes. Microchaetae clusters are abolished by expression of N^icd.hs at 7-9 hours APF.

(Pan and Rubin, 1997)

A greater proportion of the embryo has developed as neural tissue that wild type, hypertrophy of the nervous system. Most embryos show no cuticle.

(Rooke et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

kuz^e29-4 has increased cell number | embryonic stage phenotype, enhanceable by Df(3L)ED225/Df(3L)ED225

(Bivik et al., 2016)

kuz^e29-4 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by Df(3L)ED225/Df(3L)ED225

(Bivik et al., 2016)

kuz^e29-4 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by pros¹⁷

(Ulvklo et al., 2012)

kuz^e29-4 has increased cell number | embryonic stage phenotype, enhanceable by pros¹⁷

(Ulvklo et al., 2012)

kuz^e29-4 has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by robo[+]/sli[+]/robo1⁵/sli¹

(Coleman et al., 2010)

Suppressed by

Statement

Reference

kuz^e29-4 has increased cell number | embryonic stage phenotype, suppressible by dap^UAS.cUa/Scer\GAL4^elav.PU

(Bivik et al., 2016)

kuz^e29-4 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by dap^UAS.cUa/Scer\GAL4^elav.PU

(Bivik et al., 2016)

Enhancer of

Statement

Reference

kuz[+]/kuz^e29-4 is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

kuz[+]/kuz^e29-4 is an enhancer of visible phenotype of Scer\GAL4^A9, uif^asterisk.UAS

(Xie et al., 2012)

kuz^e29-4 is an enhancer of abnormal neuroanatomy | embryonic stage 16 | dominant phenotype of robo1⁵, sli[+]/sli¹

(Coleman et al., 2010)

kuz^e29-4 is an enhancer of visible phenotype of Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

Other

Statement

Reference

Df(3R)BSC751/+, kuz^e29-4 has abnormal neuroanatomy | embryonic stage phenotype

(Bivik et al., 2016)

Df(3R)BSC751/+, kuz^e29-4 has increased cell number | embryonic stage phenotype

(Bivik et al., 2016)

dap[+]/dap⁷⁸⁶⁷, kuz^e29-4 has abnormal neuroanatomy | embryonic stage phenotype

(Bivik et al., 2016)

dap[+]/dap⁷⁸⁶⁷, kuz^e29-4 has increased cell number | embryonic stage phenotype

(Bivik et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

kuz^e29-4 has larval EL neuron | embryonic stage | increased number phenotype, enhanceable by Df(3L)ED225/Df(3L)ED225

(Bivik et al., 2016)

kuz^e29-4 has apterous-expressing neuron of the lateral cluster of the thorax phenotype, enhanceable by pros¹⁷

(Ulvklo et al., 2012)

kuz^e29-4 has larval longitudinal connective | embryonic stage 16 phenotype, enhanceable by robo[+]/sli[+]/robo1⁵/sli¹

(Coleman et al., 2010)

kuz^e29-4 has commissure | embryonic stage 16 phenotype, enhanceable by robo[+]/sli[+]/robo1⁵/sli¹

(Coleman et al., 2010)

NOT Enhanced by

Statement

Reference

kuz^e29-4 has apterous-expressing neuron of the lateral cluster of the thorax | embryonic stage | increased number phenotype, non-enhanceable by Df(3L)ED225/Df(3L)ED225

(Bivik et al., 2016)

Suppressed by

Statement

Reference

kuz^e29-4 has larval EL neuron | increased number | embryonic stage phenotype, suppressible by dap^UAS.cUa/Scer\GAL4^elav.PU

(Bivik et al., 2016)

kuz^e29-4 has apterous-expressing neuron of the lateral cluster of the thorax | increased number | embryonic stage phenotype, suppressible by dap^UAS.cUa/Scer\GAL4^elav.PU

(Bivik et al., 2016)

kuz^e29-4 has presumptive embryonic/larval central nervous system | germline clone phenotype, suppressible by N^{ΔLNR.lexA.UAS}/Scer\GAL4^da.G32

(Lieber et al., 2002)

kuz^e29-4 has presumptive embryonic/larval central nervous system | germline clone phenotype, suppressible by N^lexA.UAS/Scer\GAL4^da.G32

(Lieber et al., 2002)

kuz^e29-4 has presumptive embryonic/larval central nervous system | germline clone phenotype, suppressible by N^{ΔLN.lexA.UAS}/Scer\GAL4^da.G32

(Lieber et al., 2002)

Enhancer of

Statement

Reference

kuz[+]/kuz^e29-4 is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

kuz[+]/kuz^e29-4 is an enhancer of wing vein phenotype of Scer\GAL4^A9, uif^asterisk.UAS

(Xie et al., 2012)

kuz^e29-4 is an enhancer of larval longitudinal connective | embryonic stage 16 phenotype of robo1⁵, sli[+]/sli¹

(Coleman et al., 2010)

kuz^e29-4 is an enhancer of commissure | embryonic stage 16 phenotype of robo1⁵, sli[+]/sli¹

(Coleman et al., 2010)

kuz^e29-4 is an enhancer of wing vein L3 phenotype of Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

kuz^e29-4 is an enhancer of anterior scutellar bristle phenotype of Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

NOT Enhancer of

Statement

Reference

kuz[+]/kuz^e29-4 is a non-enhancer of eye phenotype of Scer\GAL4^hs.2sev, hbs^VDRC.cUa

(Singh and Mlodzik, 2012)

kuz[+]/kuz^e29-4 is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, hbs^VDRC.cUa

(Singh and Mlodzik, 2012)

Suppressor of

Statement

Reference

kuz^e29-4 is a suppressor | partially of presumptive embryonic/larval central nervous system phenotype of N^{ΔLNR.lexA.UAS}, Scer\GAL4^da.G32

(Lieber et al., 2002)

kuz^e29-4 is a suppressor | partially of presumptive embryonic/larval central nervous system phenotype of N^{ΔLN.lexA.UAS}, Scer\GAL4^da.G32

(Lieber et al., 2002)

NOT Suppressor of

Statement

Reference

kuz[+]/kuz^e29-4 is a non-suppressor of eye phenotype of Scer\GAL4^hs.2sev, hbs^VDRC.cUa

(Singh and Mlodzik, 2012)

kuz[+]/kuz^e29-4 is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, hbs^VDRC.cUa

(Singh and Mlodzik, 2012)

kuz^e29-4 is a non-suppressor of presumptive embryonic/larval central nervous system phenotype of N^{Intra1790.lexA.UAS}, Scer\GAL4^da.G32

(Lieber et al., 2002)

Other

Statement

Reference

Df(3L)ED225, kuz^e29-4 has thoracic neuroblast phenotype

(Bivik et al., 2016)

Df(3L)ED225, kuz^e29-4 has abdominal neuroblast phenotype

(Bivik et al., 2016)

Df(3R)BSC751/+, kuz^e29-4 has larval EL neuron | embryonic stage phenotype

(Bivik et al., 2016)

Df(3R)BSC751/+, kuz^e29-4 has apterous-expressing neuron of the lateral cluster of the thorax | embryonic stage phenotype

(Bivik et al., 2016)

kuz^e29-4, pros¹⁷ has larval thoracic NB5-6 primary interneuron phenotype

(Ulvklo et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

kuz^e29-4, Df(3R)BSC620 double homozygous embryos do not show any obvious somatic musculature phenotype, as compared to controls.

(Jacobs, 2018.7.9)

kuz^e29-4/kuz^e29-4, Df(3L)ED225/Df(3L)ED225 double mutant embryos display a similar degree of supernumerary Ap neurons, but an enhanced supernumerary EL neuron phenotype, as compared to kuz^e29-4/kuz^e29-4 single mutants. These double mutants also display increased proliferation of thoracic neuroblasts at stage 15 (but not stage 12 or 14), thoracic neuroblast daughter cells at stage 14 and 15 (but not stage 12), and abdominal neuroblasts and neuroblast daughter cells at stage 14 and 15 (but not stage 12), as compared with controls.

kuz^e29-4/+, dap⁷⁸⁶⁷/+ double mutant embryos display a significant increase in the number of Ap neurons and EL neurons, and show increased proliferation of neuroblast daughter cells, but not neuroblasts, as compared to controls.

kuz^e29-4/+, Df(3R)BSC751/+ double mutant embryos display a significant increase in the number of Ap neurons, as compared to controls.

Expression of dap^Scer\UAS.cUa under the control of Scer\GAL4^elav.PU partially suppresses the increased Ap neuron number phenotype, and fully suppresses the increased EL neuron number phenotype, and suppresses the increased daughter cell proliferation of kuz^e29-4/kuz^e29-4 mutants.

(Bivik et al., 2016)

kuz^e29-4/+ has no detectable effect on the eye-phenotype resulting from the expression of hbs^VDRC.cUa under the control of Scer\GAL4^hs.2sev.

(Singh and Mlodzik, 2012)

kuz^e29-4 ; pros¹⁷ double mutant embryos show extensive overproliferation of the entire NB5-6T lineage, with an average of 69 cells. There is an increase in the number of "Ap" neurons generated in the NB5-6T lineage in the double mutant embryos compared to kuz^e29-4 single mutant embryos.

(Ulvklo et al., 2012)

The wing vein phenotype resulting from the expression of uif^{asterisk.Scer\UAS} under the control of Scer\GAL4^A9 is enhanced by kuz^e29-4.

(Xie et al., 2012)

A kuz^e29-4 background enhances the FasII axon midline crossing phenotype seen in sli¹, robo⁵ transheterozygous stage 16 embryos.

(Coleman et al., 2010)

The addition of N^{Scer\UAS.ΔLN.T:Ecol\lexA} or N^{Scer\UAS.ΔLNR.T:Ecol\lexA} suppresses the neural hypertrophy seen in kuz^e29-4 embryos leading to an identifiable central nervous system.

(Lieber et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

5804

108432

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Kuzbanian^e29-4

(Sood et al., 2022)

kuz^29-4

(Albrecht et al., 2006)

kuz^E29-4

(Albrecht et al., 2006)

kuz^E29

(Meyer and Aberle, 2006)

kuz^e29-4

(Sood et al., 2022, Salazar et al., 2021, Feuillette et al., 2020, Fic et al., 2019, Petri et al., 2019, Lucchetta and Ohlstein, 2017, Bivik et al., 2016, Bivik et al., 2015, Baumgardt et al., 2014, Singh and Mlodzik, 2012, Ulvklo et al., 2012, Xie et al., 2012, Albrecht et al., 2006, Kamimura et al., 2004, Kamimura et al., 2004)

kuz^e29

(Coleman et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (22)

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