Amino acid replacement: E?K.
Mutation maps within the second ATP binding site of the carbamyl phosphate synthetase domain of r (the CPS.B2 subdomain).
Nucleotide substitution: G?A.
G16659960A
G?A
E1167K | r-PA; E1167K | r-PD; E1167K | r-PE
E?K
Position of mutation on reference sequence inferred by FlyBase curator based on author statement (see Figure 2). Same nucleotide substitution as rSu(b)
Sensitive to beta-alanine; there is almost 100% lethality at a concentration of 0.2M, with very few first instar larvae, and large numbers of unhatched eggs, suggesting that lethality occurs early in development, and may have a maternal component. Unhatched eggs do not melanise.
bunspecified, fs(1)pyrSu(b), rSu(b)DK7 has lethal | recessive | embryonic stage phenotype
rSu(b)DK7 is a suppressor of phenotype of bunspecified
Embryos that are homozygous for fs(1)pyrSu(b) rSu(b)DK7 and bunspecified and originate from a female homozygous for these 3 mutations show severely reduced viability. Newly laid eggs begin development normally, and early embryogenesis proceeds normally through the syncytial blastoderm, gastrulation and extended germ band stages. Abnormalities such as shrinkage of the embryo and lack of muscle contraction are seen near the end of embryogenesis and the majority of embryos die just before the eggs are due to hatch.