FlyBase Allele Report: Dmel\14-3-3ε[S-696]

General Information

Symbol

Dmel\14-3-3ε^S-696

Species

D. melanogaster

Name

FlyBase ID

FBal0048994

Feature type

allele

Associated gene

Dmel\14-3-3ε

Associated Insertion(s)

Carried in Construct

Also Known As

14-3-3ε^E183K

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Benton et al., 2002)

Mutagen

ethyl methanesulfonate

(Chang and Rubin, 1997, Karim et al., 1996)

Progenitor genotype

Cytology

Description

Amino acid replacement: E183K.

(Chang and Rubin, 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:18,247,983..18,247,983 [+]

Nucleotide change:

G18247983A

Amino acid change:

E183K | 14-3-3epsilon-PA; E183K | 14-3-3epsilon-PB; E183K | 14-3-3epsilon-PC; E183K | 14-3-3epsilon-PD

Reported amino acid change:

E183K

(Chang and Rubin, 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates viral infectious disease

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

ameliorates infectious mononucleosis

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

ameliorates Epstein-Barr virus hepatitis

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

ameliorates cancer

modeled by Alk^UAS.cLa

(Wolfstetter et al., 2020)

modeled by Hsap\ALK^F1174L.UAS

(Wolfstetter et al., 2020)

modeled by Hsap\ALKAL1^UAS.cGa

(Wolfstetter et al., 2020)

modeled by Hsap\ALK::Hsap\NPM1^UAS.cWa

(Wolfstetter et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal

(Karim et al., 1996)

partially lethal | recessive

(Chang and Rubin, 1997)

visible (with 14-3-3ε^j2B10)

(Chang and Rubin, 1997)

Phenotype Manifest In

eye (with 14-3-3ε^j2B10)

(Chang and Rubin, 1997)

eye photoreceptor cell

(Chang and Rubin, 1997)

eye photoreceptor cell (with 14-3-3ε^j2B10)

(Chang and Rubin, 1997)

ommatidium

(Chang and Rubin, 1997)

ommatidium (with 14-3-3ε^j2B10)

(Chang and Rubin, 1997)

ommatidium (with Df(3R)P14)

(Chang and Rubin, 1997)

oocyte

(Benton et al., 2002)

posterior crossvein

(Chang and Rubin, 1997)

Detailed Description

Statement

Reference

14-3-3ε^S-696 mutant females have oocyte determintaion and polarity defects with a much higher penetrance than 14-3-3ε^j2B10, suggesting that this allele is antimorphic (dominant negative).

(Benton et al., 2002)

62.5% of ommatidia are normal in homozygous escapers, with the remaining ommatidia lacking some photoreceptor cells, and 69.1% of ommatidia are normal in 14-3-3ε^S-696/Df(3R)P14 flies. Homozygous escapers often have gaps in the posterior crossveins of the wings. 14-3-3ε^S-696/14-3-3ε^j2B10 flies have rough eyes and a low penetrance of missing photoreceptors in the ommatidia.

(Chang and Rubin, 1997)

Homozygotes are subviable.

(Karim et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

14-3-3ε^S-696, Raf¹² has lethal | dominant phenotype

(Chang and Rubin, 1997)

Phenotype Manifest In

Suppressor of

Statement

Reference

14-3-3ε^S-696 is a suppressor of eye phenotype of EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

14-3-3ε^S-696/14-3-3epsilon[+] is a suppressor of eye phenotype of Ras85D^V12.sev

(Chang and Rubin, 1997)

14-3-3ε^S-696 is a suppressor of eye phenotype of Raf::tor^13D.hs.sev

(Chang and Rubin, 1997)

14-3-3ε^S-696 is a suppressor of phenotype of Ras85D^V12.sev

(Karim et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

14-3-3ε^S-696 does not show any dominant interaction with aop^yan-1 or phyl^hs.sev.

(Chang and Rubin, 1997)

Suppresses the rough eye phenotype of Ras85D^V12.sev.

(Karim et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

14-3-3ε^j2B10

(Chang and Rubin, 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6565

14-3-3ε^S-696/TM3, P{sevRas1.V12}FK2, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

14-3-3epsilon^s-969

(Adamson and Lajeunesse, 2012)

14-3-3ε^E183K

(Benton et al., 2002, Chang and Rubin, 1997)

14-3-3ε^S-696

(Wolfstetter et al., 2020)

SR3-9⁶⁹⁶

(Chang and Rubin, 1997)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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